Qingjie decoction attenuated E.coli-induced diarrhea by regulating energy metabolism and alleviating inflammation based on network analysis and metabolomics

Yang, Fan; Zhao, Qingyu; Wei, Yuanyuan; Wang, Huiru; Ga, Yu; Zhang, Yannan; Hao, Zhihui

doi:10.1016/j.jep.2023.116806

Cited by 2 publications

(1 citation statement)

References 47 publications

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“…It has been reported that diarrheal disease is responsible for 370,000 children's annual mortality worldwide (Pokharel et al, 2023). In addition, E. coli causes peritonitis, pneumonia, urinary tract infections (UTIs), and urinary bacteremia (Fan et al, 2023;Sinnott-Stutzman & Sykes, 2021). Despite E. coli being the cause of those diseases, a recent emerging concern globally is antimicrobial resistance, which is among the top ten public health threats resulting from antibiotic overuse for human treatments, in animals, food production, and disposal to the environment (Kusi et al, 2022;Salam et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Assessment of resistant Escherichia coli in groundwater sources and sanitary inspection for contamination risk in Bagamoyo, Tanzania

Md,

Msuya,

Rugaika

et al. 2024

Preprint

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Background Two billion people globally are using faecal-contaminated water. E. coli poses a health risk due to its potential to cause diseases which highlights the need for understanding its antimicrobial resistance profile and associated factors for contamination. Methods A cross-sectional study was conducted in the Bagamoyo district council that involved 163 groundwater sources with sanitary inspection and isolation of E. coli was conducted using a Membrane filtration method and CHROMagar CCA. In this study, antibiotic susceptibility was carried out according to CLSI guidelines by disk diffusion methods. E. coli strain ATCC 25922 was used as the control strain in isolation and susceptibility tests. Results The current study found that 44.8% of groundwater samples were contaminated with E. coli, with 83.6% of isolates were resistant to antibiotics. High resistance was observed to cefazolin (56.16%), nitrofurantoin (54.79%), amoxicillin-clavulanic acid (45.21%), and ceftriaxone (42.47%). Also, the study reveals that 70% of E. coli isolates had multidrug resistance. On the other hand, sanitary inspection showed that 46.2% of dug wells were at a high sanitary risk level, 32.8% of tube wells with hand pumps were at High and low sanitary risk level, respectively and 73.3% of boreholes were at a low sanitary risk level. However, the study showed a high rate of E. coli contamination in boreholes at a low sanitary risk level. Also, tube wells with hand pumps and dug wells at high sanitary risk levels had a high rate of E. coli contamination. Moreover, resistant E. coli in groundwater across varying contamination risk levels revealed a significant prevalence of contamination. Conclusion These findings indicate that there is a potential high prevalence of antibiotic-resistant E. coli in groundwater in the study area and thus the need for advocacy of proper use of antibiotics and to avoid environmental contamination of resistant bacteria in groundwater sources through improved hygiene.

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Section: Introductionmentioning

confidence: 99%

Assessment of resistant Escherichia coli in groundwater sources and sanitary inspection for contamination risk in Bagamoyo, Tanzania

Md,

Msuya,

Rugaika

et al. 2024

Preprint

View full text Add to dashboard Cite

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Lactobacillus salivarius and Berberine Alleviated Yak Calves’ Diarrhea via Accommodating Oxidation Resistance, Inflammatory Factors, and Intestinal Microbiota

He,

Lu,

Wang

et al. 2024

Animals

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Yaks are important food animals in China; however, bacterial diarrheal diseases frequently occur on the plateau, with limited effective therapies. The objective of this research was to evaluate the effectiveness of Lactobacillus salivarius (LS) and berberine in alleviating diarrhea in yak calves. For this purpose, eighteen healthy yak calves were divided into control (JC), infected (JM), and treatment (JT) groups. Yaks in the JT group were treated with 2 × 1010 CFU/calf L. salivarius and 20 mg/kg berberine, and yaks in the JM and JT groups were induced with multi-drug-resistant Escherichia coli. The results showed that the weight growth rate in the JM group was significantly lower than that in the JC and JT groups. The diarrhea score in the JM group was significantly higher than that in both the JC and JT groups. Additionally, the contents of T-AOC, SOD, GSH-Px, and IL-10 were significantly lower in the JM group than those in the JC and JT groups, while MDA, TNF-α, IL-1β, and IL-6 were significantly higher in the JM group. Microbiota sequencing identified two phyla and twenty-seven genera as significant among the yak groups. Notably, probiotic genera such as Faecalibaculum and Parvibacter were observed, alongside harmful genera, including Marvinbryantia and Lachnospiraceae UCG-001. Our findings indicate that treatment with L. salivarius and berberine significantly reduced diarrhea incidence, improved growth performance, and positively modulated intestinal microbiota, which could provide novel insights for developing new therapies for ruminant diarrhea.

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Qingjie decoction attenuated E.coli-induced diarrhea by regulating energy metabolism and alleviating inflammation based on network analysis and metabolomics

Cited by 2 publications

References 47 publications

Assessment of resistant Escherichia coli in groundwater sources and sanitary inspection for contamination risk in Bagamoyo, Tanzania

Assessment of resistant Escherichia coli in groundwater sources and sanitary inspection for contamination risk in Bagamoyo, Tanzania

Lactobacillus salivarius and Berberine Alleviated Yak Calves’ Diarrhea via Accommodating Oxidation Resistance, Inflammatory Factors, and Intestinal Microbiota

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