QIAGEN DNeasy Power Water SOP v1

Abstract

QIAGEN DNeasy PowerWater SOP Purpose: For the isolation of genomic DNA from filter water samples, inculding turbid water Introduction: The DNeasy PowerWater Kit can isolate genomic DNA from a variety of filtered water samples. Utilizing Inhibitor Removal Technology® (IRT), even water containing heavy amounts of contaminants can be processed to provide DNA of high quality and yield. The DNeasy PowerWater Kit can isolate DNA equally well from any commonly used type of filter membrane. Purified DNA is ready to use in a final elution volume of 100 μl. Principle & Procedure: The DNeasy PowerWater Kit starts with the filtration of a water sample onto a filter membrane. Filter membranes may be user supplied. The membrane is then added to our special 5 ml bead beating tube containing a unique bead mix. Rapid and thorough lysis occurs through vortexing in a specially formulated lysis buffer that enhances the isolation of microorganisms from filter membranes. After the protein and inhibitor removal steps, total genomic DNA is captured on an MB Spin Column. High-quality DNA is then washed and eluted from the MB Spin Column membrane for use in downstream applications including PCR and qPCR.

Genetic and Structural Diversity of Prokaryotic Ice-Binding Proteins from the Central Arctic Ocean

“…The DNA was extracted at the Alfred Wegener Institute, using the Qiagen PowerWater DNA kit, following a slightly modified version of the QIAGEN DNeasy Power Water SOP v1 (QIAGEN N.V., Hilden, Germany) [31]. Samples were sent to the DoE Joint Genome Institute (JGI) for sequencing.…”