qF-SSOP: real-time optical property corrected fluorescence imaging

Valdés, Pablo; Angelo, Joseph; Choi, Hak; Gioux, Sylvain

doi:10.1364/boe.8.003597

Cited by 40 publications

(40 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While the current processing time to invert diffuse reflectance maps to optical properties is typically on the order of 1 second, we recently demonstrated that this inversion time could be reduced significantly to approximately 10 ms [31]. Altogether this work lays the foundation of real-time quantitative optical imaging through an endoscope and its clinical translation, potentially enabling endoscopic implementation of current quantitative, widefield techniques [32][33][34].…”

Section: Discussionmentioning

confidence: 85%

Real-time endoscopic optical properties imaging

Angelo¹,

Giessen²,

Gioux³

2017

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Abstract:With almost 50% of all surgeries in the U.S. being performed as minimally invasive procedures, there is a need to develop quantitative endoscopic imaging techniques to aid surgical guidance. Recent developments in widefield optical imaging make endoscopic implementations of real-time measurement possible. In this work, we introduce a proof-ofconcept endoscopic implementation of a functional widefield imaging technique called 3D single snapshot of optical properties (3D-SSOP) that provides quantitative maps of absorption and reduced scattering optical properties as well as surface topography with simple instrumentation added to a commercial endoscope. The system's precision and accuracy is validated using tissue-mimicking phantoms, showing a max error of 0.004 mm −1 , 0.05 mm −1 , and 1.1 mm for absorption, reduced scattering, and sample topography, respectively. This study further demonstrates video acquisition of a moving phantom and an in vivo sample with a framerate of approximately 11 frames per second.

show abstract

Section: Discussionmentioning

confidence: 85%

Real-time endoscopic optical properties imaging

Angelo¹,

Giessen²,

Gioux³

2017

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…Quantitative fluorescence means absolute numbers can be attributed to signal for diagnostic purposes. Valdes et al 75 recently applied the principles of real-time optical properties imaging (SSOP) to the quantitative measurement of fluorescence (termed qF-SSOP), allowing to predict fluorescence concentration within 5% in various turbid media optical properties, and in real time, making it amenable to be used during surgery [ Fig. 8(a)].…”

Section: Sfdi and Fluorescencementioning

confidence: 99%

“…23,25,73,108 Recent work by Valdes et al has prototyped a real-time fluorescence measurement embodiment to facilitate translation to operating room and real-time scenarios. 75 SFDIderived subdiffusive endogenous reflectance and scattering contrast has also become a useful tool for classifying excised tissue types. The main goal for this is work is to enable intraoperative assessment of lumpectomy margins by differentiating between normal, fibroadenoma, ductal carcinoma in situ (DCIS), and invasive cancer.…”

Section: Oncology/cancermentioning

confidence: 99%

“…Simply combining SFDI-derived optical properties with other modalities such fluorescence, laser speckle imaging, multiphoton imaging, multispectral imaging, and Cerenkov can enhance the quantitative nature of each modality. 75,84,88,132 Finally, SFDI applications extend to wherever turbidity is an issue-including nonmedical applications. As an example, multiple groups are using SFDI to characterize produce and identify subsurface bruising in apples and pears as a quality control tool.…”

Section: Turbid Sample Characterizationmentioning

confidence: 99%

See 1 more Smart Citation

Spatial frequency domain imaging in 2019: principles, applications, and perspectives

2019

Self Cite

View full text Add to dashboard Cite

Spatial frequency domain imaging (SFDI) has witnessed very rapid growth over the last decade, owing to its unique capabilities for imaging optical properties and chromophores over a large field-of-view and in a rapid manner. We provide a comprehensive review of the principles of this imaging method as of 2019, review the modeling of light propagation in this domain, describe acquisition methods, provide an understanding of the various implementations and their practical limitations, and finally review applications that have been published in the literature. Importantly, we also introduce a group effort by several key actors in the field for the dissemination of SFDI, including publications, advice in hardware and implementations, and processing code, all freely available online.

show abstract

“…13,14 For instance, fluorescence imaging to help surgeons visualizing structures of interest, such as lymph nodes, ureters, or micrometastasis, stands as an example where providing tissue-related information in real time plays a key role in clinical adoption. [15][16][17][18] Among all existing solutions, spatial frequency domain imaging (SFDI) has been recently developed with the unique capacity to provide rapidly quantitative diffuse information (absorption and reduced scattering) over large fields of view (typically, >10 × 10 cm 2 ). 14,19 Even more recently, the SFDI method has been improved to image optical properties more rapidly using two images 20 or even a single image.…”

Section: Introductionmentioning

confidence: 99%

Single snapshot of optical properties image quality improvement using anisotropic two-dimensional windows filtering

et al. 2019

Self Cite

View full text Add to dashboard Cite

Enagnon Aguénounon, Foudil Dadouche, Wilfried Uhring, Sylvain Gioux, "Single snapshot of optical properties image quality improvement using anisotropic two-dimensional windows filtering," J.Abstract. Imaging methods permitting real-time, wide-field, and quantitative optical mapping of biological tissue properties offer an unprecedented range of applications for clinical use. Following the development of spatial frequency domain imaging, we introduce a real-time demodulation method called single snapshot of optical properties (SSOPs). However, since this method uses only a single image to generate absorption and reduced scattering maps, it was limited by a degraded image quality resulting in artifacts that diminished its potential for clinical use. We present filtering strategies for improving the image quality of optical properties maps obtained using SSOPs. We investigate the effect of anisotropic two-dimensional filtering strategies for spatial frequencies ranging from 0.1 to 0.4 mm −1 directly onto N ¼ 10 hands. Both accuracy and image quality of the optical properties are quantified in comparison with standard, multiple image acquisitions in the spatial frequency domain. Overall, using optimized filters, mean errors in predicting optical properties using SSOP remain under 8.8% in absorption and 7.5% in reduced scattering, while significantly improving image quality. Overall this work contributes to advance real-time, wide-field, and quantitative diffuse optical imaging toward clinical evaluation.

show abstract

qF-SSOP: real-time optical property corrected fluorescence imaging

Cited by 40 publications

References 25 publications

Real-time endoscopic optical properties imaging

Real-time endoscopic optical properties imaging

Spatial frequency domain imaging in 2019: principles, applications, and perspectives

Single snapshot of optical properties image quality improvement using anisotropic two-dimensional windows filtering

Contact Info

Product

Resources

About