QCM-D studies of attachment and differential spreading of pre-osteoblastic cells on Ta and Cr surfaces

Modin, Charlotte; Stranne, Anne-Louise; Foss, Morten; Duch, Mogens; Justesen, Jeannette; Chevallier, J.; Andersen, Lars Klembt; Hemmersam, Anne Gry; Pedersen, Finn Skou; Besenbacher, Flemming

doi:10.1016/j.biomaterials.2005.09.022

Cited by 100 publications

(82 citation statements)

References 33 publications

(60 reference statements)

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“…For example, Modin et al (2006) studied initial osteoblast adhesion by using a quartz crystal microbalance with dissipation (QCM-D). However, this technique is not appropriate to analyse the adhesion strength of single cells.…”

Section: Discussionmentioning

confidence: 99%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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Organised nanotopography mimicking the natural extracellular matrix can be used to control morphology, cell motility, and differentiation. However, it is still unknown how specifi c cell types react with specifi c patterns. Both initial adhesion and preferential cell migration may be important to initiate and increase cell locomotion and coverage with cells, and thus achieve an enhanced wound healing response around an implantable material. Therefore, the aim of this study was to evaluate how MC3T3-E1 osteoblast initial adhesion and directional migration are infl uenced by nanogrooves with pitches ranging from 150 nm up to 1000 nm. In this study, we used a multipatterned substrate with fi ve different groove patterns and a smooth area with either a concentric or radial orientation. Initial cell adhesion measurements after 10 s were performed using atomic force spectroscopy-assisted single-cell force spectroscopy, and demonstrated that nascent cell adhesion was highly induced by a 600 nm pitch and reduced by a 150 nm pitch. Addition of RGD peptide signifi cantly reduced adhesion, indicating that integrins and cell adhesive proteins (e.g. fi bronectin or vitronectin) are key factors in specifi c cell adhesion on nanogrooved substrates. Also, cell migration was highly dependent on the groove pitch; the highest directional migration parallel to the grooves was observed on a 600 nm pitch, whereas a 150 nm pitch restrained directional cell migration. From this study, we conclude that grooves with a pitch of 600 nm may be favourable to enhance fast wound closure, thereby promoting tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Dynamic cell adhesion and migration on nanoscale grooved substrates

Lamers

Riet²,

Domański³

et al. 2012

eCM

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show abstract

“…In this strategy, it is also important to have a good understanding of the adsorption phenomena on both dental materials and teeth [1][2][3] . Adsorbates may range from small organic adhesive monomers 4,5) to large polymers 6) or cells 7) , and the mechanisms underlying adsorption are diverse. Antibacterial agents may be artificial synthetic drugs or natural secreted substances.…”

Section: Introductionmentioning

confidence: 99%

Effect of pH and Addition of Salt on the Adsorption Behavior of Lysozyme on Gold, Silica, and Titania Surfaces Observed by Quartz Crystal Microbalance with Dissipation Monitoring

et al. 2008

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The adsorption behaviors of lysozyme on dentally related Au, SiO2, and TiO2 surfaces were investigated by a quartz crystal microbalance with dissipation monitoring (QCM-D) method. Frequency shifts indicated that while lysozyme (pI 11) was fairly adsorbed on the SiO2 (pI 1.9) surface at both pH 3 and 7, it was adsorbed on TiO2 (pI 6.3) surface only at pH 7. However, adsorption was disturbed by 50 mM NaCl. These results strongly suggested an electrostatic nature of the adsorption behavior. Though a large-scale adsorption of the lysozyme on Au sensor was pH-insensitive, softness of the adlayer as seen from the dissipation profile was pH-dependent, indicating an interaction of another type. With all the surfaces, the small dissipation change indicated a stiff lysozyme adlayer. Results of this study revealed that the controlled electrostatic interaction between the material surface and lysozyme might be a useful method for imparting antibacterial property to the dental materials.

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“…Specifically, QCM allows us to monitor the attachment and spread of cells on a particular surface quantitatively in real-time, with no need for destructive interventions. Several surface-adherent cell types have been examined (Marx Kenneth et al, 2003), and various parameters are measured to evaluate the viscoelastic properties of cells adhered to QCM crystal besides frequency shift, such as dissipation, resistance, inductance and transient decay time constants oscillation (Modin et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Cell adhesion on Ti surface with controlled roughness

et al. 2015

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ABSTRACT:In this report, the in situ interaction between Saos-2 osteoblast cells and a smooth Ti surface was examined over time. The adhesion kinetics and mechanisms of cellular proliferation were monitored by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). The rate of Saos-2 attachment on Ti surfaces, obtained from the measurements performed with the QCM, is a first-order reaction, with k=2.10. The impedance measurements indicate that in the absence of cells, the Ti resistance diminishes over time (7 days), due to the presence of amino acids and proteins from the culture medium that have been a dsorbed, while in the presence of osteoblasts, this decrease is much greater because of the compounds generated by the cells that accelerate the dissolution of Ti. RESUMEN: Adhesión de osteoblastos sobre una superficie de Ti de rugosidad controlada.En este trabajo, se ha estudiado la interacción in situ entre células osteoblásticas Saos-2 y una superficie de Ti de rugosidad controlada a lo largo del tiempo. El estudio de la cinética y los mecanismos de proliferación celular de adhesión se ha realizado a través de la microbalanza de cristal de cuarzo (QCM) y espectroscopía de impedancia electroquímica (EIS). La velocidad de adhesión de los osteoblastos sobre la superficie de Ti obtenida a través de medidas con la QCM, sigue una reacción de primer orden, con k=2×10 −3 min −1. Los ensayos de impedancia indican que, en ausencia de las células, la resistencia del Ti disminuye con el tiempo (7 días), debido a la presencia de aminoáci-dos y proteínas del medio de cultivo que se han adsorbido, mientras que en presencia de células, esta disminución es mucho mayor debido a los productos metabólicos generados por las células que aceleran la disolución del Ti.

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QCM-D studies of attachment and differential spreading of pre-osteoblastic cells on Ta and Cr surfaces

Cited by 100 publications

References 33 publications

Dynamic cell adhesion and migration on nanoscale grooved substrates

Dynamic cell adhesion and migration on nanoscale grooved substrates

Effect of pH and Addition of Salt on the Adsorption Behavior of Lysozyme on Gold, Silica, and Titania Surfaces Observed by Quartz Crystal Microbalance with Dissipation Monitoring

Cell adhesion on Ti surface with controlled roughness

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