QCM-Based Measurement of Bond Rupture Forces in DNA Double Helices for Complementarity Sensing

Dultsev, F. N.; Kolosovsky, E.A.; Mik, Ivan A.; Lomzov, Alexander A.; Pyshnyi, D. V.

doi:10.1021/la402971a

Cited by 16 publications

(18 citation statements)

References 35 publications

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“…Harmonic voltage U 0 is smoothly increased from 0 to 7 V; the maximal voltage (7 V) was chosen on the basis of the results of previous experiments. 19 Only helix unwinding occurs within this voltage range. The time of voltage scanning was varied from 1 to 300 s.…”

Section: Methodsmentioning

confidence: 90%

“…The force value determined with the help of QCM corresponds to the unzipping mode, which is in good agreement with the model proposed previously. 19 …”

Section: Methodsmentioning

confidence: 99%

“…In the present work, we continue to develop the procedure proposed by us previously. 17 − 19 It was demonstrated that unwinding of the DNA double helix may be carried out with the help of quartz crystal microbalance (QCM). In particular, temperature dependence was studied previously.…”

Section: Introductionmentioning

confidence: 99%

“…This comparison confirmed once more that unwinding proceeds according to the mechanism proposed by us in our previous work. 19 …”

Section: Introductionmentioning

confidence: 99%

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Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods

Kurus

Dultsev

2018

ACS Omega

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For the first time, rupture event scanning (REVS) procedure based on quartz crystal microbalance (QCM) and involving only mechanical action was used to determine the height of the energy barrier for dsDNA unwinding. Melting point was determined with the help of this procedure. To determine the thermodynamic parameters including enthalpy, DNA denaturation was represented as a unimolecular process. This allowed us to recover the energy profiles from the experimental data obtained by force measurements at different scanning times (reaction times) for different temperatures. The thus obtained results were compared with the data obtained with the help of another mechanical method, namely, atomic force microscopy. The mechanism of DNA unwinding in QCM-based experiments through the unzipping mode, as proposed by us in previous works, was confirmed. Thus, we demonstrated that REVS procedure may be used to assess the thermodynamic parameters of dsDNA unwinding.

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Section: Methodsmentioning

confidence: 90%

“…The force value determined with the help of QCM corresponds to the unzipping mode, which is in good agreement with the model proposed previously. 19 …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…This comparison confirmed once more that unwinding proceeds according to the mechanism proposed by us in our previous work. 19 …”

Section: Introductionmentioning

confidence: 99%

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Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods

Kurus

Dultsev

2018

ACS Omega

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“…Another promising principle for creating a micromechanical sensor is an approach that makes it possible to determine the bond braking strength due without external electromagnetic action on the object, and therefore it is of great value for studying the biological objects, such as nucleic acids [89,90]. The technique is based on the use of quartz crystal unit as a sensor that also plays an active role with respect to the particle fastened off on the surface thereof.…”

Section: Micromechanical (Piezoelectric) Sensorsmentioning

confidence: 99%

Existing Biosensing Platforms for the Nucleotide Markers Detection

Dmitrienko

Pyshnaya

Dultsev

et al. 2016

Preprint

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Abstract:In review, the operating principles of the most common bio sensing devices, detection methods and the identification sensitivity of analyzed molecules were shown. The central focus was done on hybridization analysis of nucleic acids (NA), which are considered to be one of the most important analytes in terms of diagnostic point of view. Constructions enabling to transfer the fact of formation of nucleotide probe/target complex in to detectable signal by optical, electrochemical or micromechanical (piezoelectric) sensors were presented in this review.

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Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors

Oberleitner

2018

Springer Theses

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These cellular responses and the accompanying changes of physicochemical parameters are what is to be detected and transferred into a measurable electrical signal by the transducer units of the label-free biosensor devices (Fig. 1-1). There are various types of transducers, distinguishable by the underlying physicochemical phenomenon: electrochemical (including potentiometric, amperometric, and impedimetric), optical, thermometric, piezoelectric, and magnetic transduction. [8] Tab. 1-1 gives an LAPSLight-addressable potentiometric sensors (LAPSs) are multilayer field-effect devices similar to ISFETs [66] . A LAPS consists of a doped semiconductor covered with an insulator layer and a chemosensitive layer. The device is immersed in an electrolyte solution. The semiconductor is connected with a reference electrode in contact with the electrolyte (Fig. 1-2 B; reviewed in [10,17,[67][68][69][70] with respect to biological and cell-based applications). The insulator material of LAPSs is typically made up either of a single SiO 2 layer 1.1 Label-free Biosensors for Cell-based Assays 7 chip-based systems for the label-free and continuous monitoring of DO in cell-based assays, i.e. the oxygen consumption rate (OCR) of cells. [47,48,50,53,54,57,[60][61][62] The sensor chips with integrated amperometric oxygen sensors are typically build up as multi-parametric sensors and contain further physicochemical transducer structures like e.g. for cell morphology testing and ECAR monitoring. Besides the direct measurement of DO, the amperometric sensors are also capable of sensing indirectly any species that is consumed or produced in an oxidase-based enzymatic reaction (amperometric enzyme sensor) via the oxygen or hydrogen peroxide concentration. For this purpose, the amperometric pO 2 sensor is coated with a layer in which the respective enzyme is immobilized and which enables free diffusion of all reactants between the bulk solution and the sensor surface. This approach was first presented by Clark and Lyons as an amperometric sensor for glucose [85] and has also been applied as transduction method in cell-based assays for the label-free determination and monitoring of glucose and lactose [78,79] . A list of physiologically relevant analytes that have been successfully monitored by amperometric enzyme sensors is given in [86] . ECISElectrical impedance-based sensing of adherent cells, also termed as electric cell-substrate impedance sensing (ECIS), utilizes the property of cells to impede AC current flow when they are in close contact to the substrate, i.e. a small gold-film working electrode (Fig. 1-2 D). This technique was presented first by Giaever and Keese in 1984 [87] as "morphological biosensor for mammalian cells" [88] . The principle of ECIS, the method of impedance spectroscopy, and the analysis, physiological meaning, and modeling of impedance data are extensively addressed in the methods section 3.4 Electric Cell-Substrate Impedance Sensing (ECIS). Briefly summarized, ECIS enables the time-resolved, label-free, and ...

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QCM-Based Measurement of Bond Rupture Forces in DNA Double Helices for Complementarity Sensing

Cited by 16 publications

References 35 publications

Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods

Determination of the Thermodynamic Parameters of DNA Double Helix Unwinding with the Help of Mechanical Methods

Existing Biosensing Platforms for the Nucleotide Markers Detection

Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors

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