Qa-2 expression levels is related with tumor-infiltrating lymphocytes profile during solid Ehrlich tumor development

Abstract

The Qa-2 has been described as Human Leucocyte Antigen G (HLA-G) murine homolog. This homology is well accepted to gene and protein structure, in different pathology process and embryos implantation. However, in some neoplasm, this homology is questioned, where Qa-2 has been proposed as an immunogenic molecule, associated to tumor rejection. In this way, the aim of this study was to describe the pattern of Qa-2 expression and its relationship with the profile of tumor-infiltrating lymphocytes in solid Ehrlich tumor. The Ehrlich tumor growth was evaluated in Balb/c female mice in different tumor stages. The inflammatory infiltration features were determined by histopathology and, both lymphocyte type and tissue Qa-2 expression by immunohistochemistry. ELISA kit was used to determine soluble Qa-2 in the serum from the animals. We observed that Qa-2 in neoplastic cells increases in intermediate tumor development stages, while, serum Qa-2 increases in the late stage. Qa-2 increasing is correlated with CD3+ increase. Our results suggest that Qa-2 has a role opposite to HLA-G in Ehrlich solid carcinoma, and may be modulating the immune response by attracting the inflammatory infiltrate, especially T CD8+ Lymphocytes.

P1G10, the Proteolytic Fraction from Vasconcellea cundinamarcensis, Stimulates Tissue Repair after Acute Exposure to Ultraviolet B Radiation

“…The streptavidin-peroxidase (SP) method was used for immunohistochemical staining using the Kit Ultra vision large volume anti-polyvalent detection system, HRP-Lab Vision, DAKO, as described [63]. The antibody used was Anti-caspase-3 (dilution 1:600—Cell Signaling Technology, Danvers, MA, USA).…”

Features and applications of Ehrlich tumor model in cancer studies: a literature review

“…Peritumor inflammatory infiltrates in SEC are composed of mononuclear and polymorphonuclear leukocytes, with moderate intensity between days 2 and 7 post-inoculation. Da Silva et al also showed a positive correlation between the intensity of inflammatory infiltrate and animal weight and tumor size ( 41 ). The main lymphocytes are CD3 + , CD4 + , CD8 + , and Foxp3.…”

“…The main lymphocytes are CD3 + , CD4 + , CD8 + , and Foxp3. Increases in CD3 + and Foxp3 and decreases in CD4 + and CD8 + were linked with tumor development ( 41 ). Additionally, SEC cells that were inoculated in the intraplantar area in mice were characterized by local neutrophilic infiltration with an extensive necrotic area, which may be responsible for mechanical allodynia, thermal hyperalgesia, and paw edema ( 42 ).…”

The expanding role of murine class Ib MHC in the development and activation of Natural Killer cells

“…In the case of tumours controlled by cytotoxic T cells, studies into Qa-2 expression are not clear, although current research tends to report anti-tumorigenic properties of Qa-2. In the case of 4T1 breast cancer (da Silva et al, 2017a), B16 melanoma (Chiang and Stroynowski, 2004) and Ehrlich tumours (da Silva et al, 2017b), all of which are controlled by cytotoxic T lymphocytes (CTLs), Qa-2 expression is positively correlated with tumour clearance. However, there are also two conflicting studies by the same group examining Qa-2 in NK-cell sensitive tumours.…”