Abstract:Imaging increasingly large neuronal populations at high rates pushed multi-photon microscopy into the photon-deprived regime. We present PySight, an add-on hardware and software solution tailored for photon-deprived imaging conditions. PySight more than triples the median amplitude of neuronal calcium transients in awake mice, and facilitates single-trial intravital voltage imaging in fruit flies. Its unique data streaming architecture allowed us to image a fruit fly's olfactory response over 234×600×330µm 3 a… Show more
“…This protocol guides the prospective user through the necessary steps towards laser scanning microscopy with a MCS6A multiscaler. For your convenience, we provide customized settings files tailored for planar and volumetric two-photon microscopy in Code 1 [1].…”
Section: Protocol For Volumetric Tplsm With a Mcs6a Multiscalermentioning
confidence: 99%
“…This value, as well as most others, can be iteratively improved until a satisfactory result is achieved for a given acquisition system. The full explanation on the usage of PySight and its parameters can be found in the documentation of the Python package [1].…”
Section: Converting Photon Counts To Neu-ronal Calcium Transientsmentioning
“…This protocol guides the prospective user through the necessary steps towards laser scanning microscopy with a MCS6A multiscaler. For your convenience, we provide customized settings files tailored for planar and volumetric two-photon microscopy in Code 1 [1].…”
Section: Protocol For Volumetric Tplsm With a Mcs6a Multiscalermentioning
confidence: 99%
“…This value, as well as most others, can be iteratively improved until a satisfactory result is achieved for a given acquisition system. The full explanation on the usage of PySight and its parameters can be found in the documentation of the Python package [1].…”
Section: Converting Photon Counts To Neu-ronal Calcium Transientsmentioning
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.