Pyruvic acid production using methylotrophic yeast transformants as catalyst

Eisenberg, Amy; Seip, John E.; Gavagan, John E.; Payne, Mark S.; Anton, David L.; DiCosimo, Robert

doi:10.1016/s1381-1177(96)00021-5

Cited by 42 publications

(31 citation statements)

References 12 publications

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“…These process alternatives can be divided into three different approaches using: (i) enzymes (Burdick and Schaeffer, 1987;Eisenberg et al, 1997); (ii) resting cells (Izumi et al, 1982, Ogawa et al, 2001Schinschel and Simon, 1993); and (iii) fermentation processes (Li et al, 2001a(Li et al, , 2001b(Li et al, , 2002Yokota et al, 1994). In comparison to the other approaches, fermentation methods offer the opportunity to produce pyruvate from the sustainable, lowcost substrate, glucose, with high product/substrate yield, while avoiding the coproduction of unwanted byproducts like H 2 O 2 , as is usually the case when enzymatic methods are employed.…”

Section: Introductionmentioning

confidence: 99%

Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed‐batch to in situ product recovery with fully integrated electrodialysis

Zelić

Gostović

Vuorilehto

et al. 2004

Biotech & Bioengineering

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Using the pyruvate production strain Escherichia coli YYC202 ldhA::Kan different process alternatives are studied with the aim of preventing potential product inhibition by appropriate product separation. This strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate, resulting in acetate auxotrophy during growth in glucose minimal medium. Continuous experiments with cell retention, repetitive fed-batch, and an in situ product recovery (ISPR) process with fully integrated electrodialysis were tested. Although the continuous approach achieved a high volumetric productivity (QP) of 110 g L(-1) d(-1), this approach was not pursued because of long-term production strain instabilities. The highest pyruvate/glucose molar yield of up to 1.78 mol mol(-1) together with high QP 145 g L(-1) d(-1) and high pyruvate titers was achieved by the repetitive fed-batch approach. To separate pyruvate from fermentation broth a fully integrated continuous process was developed. In this process electrodialysis was used as a separation unit. Under optimum conditions a (calculated) final pyruvate titer of >900 mmol L(-1) (79 g L(-1)) was achieved.

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Section: Introductionmentioning

confidence: 99%

Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed‐batch to in situ product recovery with fully integrated electrodialysis

Zelić

Gostović

Vuorilehto

et al. 2004

Biotech & Bioengineering

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“…at a concentration of 6 g/L (dry cells) converted 0.5 M L-lactate into pyruvate in 12 h [82]. Permeabilized yeast cells of either Hansenula polymorpha or Pichia pastoris expressing glycolate oxidase and catalase were able to oxidize 0.5 M L-lactate to pyruvate for 12 cycles with 98% conversion of the lactate [83]. An L-lactate concentration above 0.5 M showed substrate inhibition of a whole-cell biocatalyst based on recombinant P. pastoris, and oxygen, a substrate for glycolate oxidase, was important for the conversion [84].…”

Section: Other Biological Methodsmentioning

confidence: 99%

Recent Progress in the Microbial Production of Pyruvic Acid

Maleki

Eiteman

2017

Fermentation

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Pyruvic acid (pyruvate) is a cellular metabolite found at the biochemical junction of glycolysis and the tricarboxylic acid cycle. Pyruvate is used in food, cosmetics, pharmaceutical and agricultural applications. Microbial production of pyruvate from either yeast or bacteria relies on restricting the natural catabolism of pyruvate, while also limiting the accumulation of the numerous potential by-products. In this review we describe research to improve pyruvate formation which has targeted both strain development and process development. Strain development requires an understanding of carbohydrate metabolism and the many competing enzymes which use pyruvate as a substrate, and it often combines classical mutation/isolation approaches with modern metabolic engineering strategies. Process development requires an understanding of operational modes and their differing effects on microbial growth and product formation.

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“…As an important substrate, it has been widely used in enzymatic production of amino acids such as l-tyrosine, l-dihydroxyphenylalanine, and l-tryptophan [5,6]. Pyruvate is currently prepared by chemical synthesis, fermentation [7], and biocatalysis [8][9][10][11][12][13]. Due to the increasing concerns on environment-friendly processes, there is growing interest in the pyruvate production by biotechnological methods [14].…”

Section: Introductionmentioning

confidence: 99%