Pyruvic acid levels in serum and saliva: A new course for oral cancer screening?

Abstract

Objective:Cancerous cells show increased glycolysis rate. This will increase overall levels of pyruvate as it is one of the end products of glycolysis. The present on-going study is to estimate the levels of pyruvate in saliva and serum among healthy and oral cancer subjects.Settings and Design:Hospital-based cross-sectional comparative study.Methodology:A total of 50 subjects among healthy and oral cancer subjects were selected based on clinical and histological criteria. Saliva and serum samples were collected and subjected to pyruvate level estimation using biochemical analysis.Statistical Analysis:Descriptive analysis and Mann-Whitney test were used to find the statistical difference between the two independent groups.Results:Serum pyruvic acid levels of the healthy group were 1.09 ± 0.14 and for oral cancer, it was 2.95 ± 0.59 and salivary level were 3.49 ± 0.47 and 1.32 ± 0.10 respectively. Mann-Whitney test showed statistically significant difference in serum and salivary pyruvate level in between two groups (P < 0.000 respectively).Conclusion:The present study showed noticeable variation in the level of pyruvic acid among healthy and oral cancer subjects. This generates the hypothesis that estimation of the pyruvic acid can be a new tool to screening of the cancer.

Development of pyruvate oxidase-based amperometric biosensor for pyruvate determination

“…The reaction results in pyruvate oxidation with the formation of electrochemically active hydrogen peroxide. Application of a positive potential on the electrode stimulates the reaction of hydrogen peroxide decomposition (2) and generation of electrons, which are directly registered using the amperometric transducer.…”

“…The pyruvate concentration in the blood normally ranges from 40-50 to 100 μmol/L [1,2]. The pyruvate level increases when the aero bic processes intensify and at insufficient pyruvate utilization in the pyruvate dehydro- ISSN 1993-6842 (on-line); ISSN 0233-7657 (print) Biopolymers and Cell.…”

“…An increased by 2-2.8 times concentration of pyruvate is also found in the serum and saliva of oral cancer patients. The evaluation of pyruvate concentration is considered as a new method of cancer screening [2,4]. Additionally, an increased content of pyruvate in tissues typically indicates an imbalance in the systems of oxygen supply and consumption.…”

Development of Amperometric Biosensor System for Simultaneous Determination of Pyruvate and Lactate

“…Концентрація пірувату у крові у нормі скла-дає від 40-50 до 100 мкмоль/л [1], [2]. Підви-щення рівня пірувату виникає як при підвище-ному його утворенні при посиленні аеробних процесів, так і при недостатній утилізації в піруватдегідрогеназному комплексі.…”

“…Також показано збільшення концентрації пірувату у сироватці крові та слині хворих на рак ротової порожни-ни у 2-2,8 рази. Оцінка концентрації пірувату розглядається як новий метод скринінгу раку [8], [2]. Тоді як тривалий моніторинг лакта-ту застосовується в клінічній практиці невід-кладної терапії, моніторинг пірувату до сих пір не впроваджено.…”