Pyruvate prevents peroxide-induced injury of in vitro preimplantation bovine embryos

Morales, Hervé; Tilquin, Pierre; Rees, Jean-François; Massip, A.; Dessy, F.; Langendonckt, Anne Van

doi:10.1002/(sici)1098-2795(199902)52:2<149::aid-mrd5>3.0.co;2-4

Cited by 62 publications

(43 citation statements)

References 49 publications

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“…This mechanism is not clear. This study agrees with the finding that pyruvate has an antioxidants property, when in vitro cultured with bovine embryo and human spermatozoa, preventing the peroxide-induced injury (Morales et al, 1999). Ogasawara et al (1993) reported that taurine has indirect antioxidant property, which is in agreement with our study.…”

Section: Discussionsupporting

confidence: 93%

Antioxidative Effect of Pyruvate, Taurine and Melatonin, and Relationships of the Evaluation Methods on Boar Sperm Viability for In Vitro Storage

2007

Journal of Animal Science and Technology

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Section: Discussionsupporting

confidence: 93%

Antioxidative Effect of Pyruvate, Taurine and Melatonin, and Relationships of the Evaluation Methods on Boar Sperm Viability for In Vitro Storage

2007

Journal of Animal Science and Technology

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“…Most studies investigating the effect of exogenous H 2 O 2 supplementation during in vitro culture showed a negative effect on embryo development at different stages of development. H 2 O 2 incubation of bovine blastocysts (Morales et al 1999), immature rat oocytes (Chaube et al 2005), and murine zygotes resulted in decreased development and/or increased apoptosis. Bars with different superscript letters differ significantly (P!0.05).…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies in cattle and mice, H 2 O 2 supplementation to zygotes, 9-to 16-cell stage embryos, or blastocysts was shown to be detrimental for embryo development, causing excessive fragmentation and apoptosis (Morales et al 1999, Feugang et al 2004. However, data from recent studies indicate that in some cases, a shortterm period of stress can be beneficial to oocytes during maturation: 30-120 min of hydrostatic pressure improved porcine embryo development after activation (Pribenszky et al 2008a), after vitrification (Du et al 2008a, Pribenszky et al 2008a) and after handmade cloning (Du et al 2008b).…”

Section: Introductionmentioning

confidence: 97%

Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development

Vandaele¹,

Thys²,

Bijttebier³

et al. 2010

Reproduction

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Recent studies have shown that short-term exposure of oocytes to a stressor such as hydrostatic pressure or osmotic stress might induce stress tolerance in embryos. The aim of the present study was to investigate the consequences of short-term hydrogen peroxide (H 2 O 2 ) exposure to bovine in vitro matured cumulus-oocyte complexes (COCs) on subsequent preimplantation embryo development and apoptosis. In the first experiment, mature COCs were incubated in H 2 O 2 at concentrations ranging between 0.01 and 100 mmol/l, and subsequently fertilized and cultured. Oocyte incubation with 50-100 mmol/l of H 2 O 2 resulted in a significantly higher blastocyst yield (47.3%) in comparison with control medium (31.8%), while apoptotic cell ratio was inversely related with H 2 O 2 concentration. In the second experiment, we showed that the stress tolerance after H 2 O 2 exposure was not mediated by increased glutathione content in treated oocytes nor by enhanced fertilization or penetration. Further research should concentrate on the potential role of players that have been associated with stress tolerance in somatic cell lines.

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“…Furthermore, ROS-induced apoptosis has also been observed in mammalian oocytes (Tatemoto et al 2000, Chaube et al 2005) and embryos (Yang et al 1998). A possible function for pyruvate in protecting embryos against oxidative stress has been suggested (O'Fallon & Wright 1995), and it was reported that pyruvate prevented peroxide-induced injury of in vitro cultured bovine embryos (Morales et al 1999) and protected human spermatozoa against the effect of ROS (de Lamirande & Gagnon 1992). Furthermore, it has been demonstrated that pyruvate prevents ROSinduced apoptosis in various types of somatic cells (Ramakrishnan et al 1998, Kang et al 2001, Mongan et al 2002, Jagtap et al 2003), mainly by maintaining the level of GSH (Mongan et al 2002), increasing the ratio of anti-apoptotic molecules BCL2 or BCL2L1 to pro-apoptotic molecule BAX (Mongan et al 2002 and by inhibiting the activation of caspase-3 (Mongan et al 2002, Jagtap et al 2003, Lee et al 2004).…”

Section: N Liu Y-g Wu and Othersmentioning

confidence: 99%

Pyruvate prevents aging of mouse oocytes

Liu

Wu²,

Lan³

et al. 2009

Reproduction

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Inhibiting oocyte aging is important not only for healthy reproduction but also for the success of assisted reproduction techniques. Although our previous studies showed that cumulus cells accelerated aging of mouse oocytes, the underlying mechanism is unknown. The objective of this paper was to study the effects of pyruvate and cumulus cells on mouse oocyte aging. Freshly ovulated mouse cumulus-oocyte complexes (COCs) or cumulus-denuded oocytes (DOs) were cultured in Chatot-Ziomek-Bavister (CZB) medium or COC-conditioned CZB medium supplemented with different concentrations of pyruvate before being examined for aging signs and developmental potential. Pyruvate supplementation to CZB medium decreased rates of ethanol-induced activation in both COCs and DOs by maintaining their maturation-promoting factor activities, but more pyruvate was needed for COCs than for DOs. Addition of pyruvate to the COC-conditioned CZB also alleviated aging of DOs. Observations on cortical granules, level of BCL2 proteins, histone acetylation, intracellular concentration of glutathione, and embryo development all confirmed that pyruvate supplementation inhibited aging of mouse oocytes. It is concluded that the aging of mouse oocytes, facilitated by culture in COCs, can be partially prevented by the addition of pyruvate to the culture medium.

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Pyruvate prevents peroxide-induced injury of in vitro preimplantation bovine embryos

Cited by 62 publications

References 49 publications

Antioxidative Effect of Pyruvate, Taurine and Melatonin, and Relationships of the Evaluation Methods on Boar Sperm Viability for In Vitro Storage

Antioxidative Effect of Pyruvate, Taurine and Melatonin, and Relationships of the Evaluation Methods on Boar Sperm Viability for In Vitro Storage

Short-term exposure to hydrogen peroxide during oocyte maturation improves bovine embryo development

Pyruvate prevents aging of mouse oocytes

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