Pyruvate Kinase and Phosphofructokinase Isozymes in Childhood Cancers

Cottreau, Dominique; Rousseau-Merck, Marie-Françoise; Nézelof, C; Kahn, Axel

doi:10.1203/00006450-198203000-00007

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…In order to define histological and prognostic criteria for nephroblastoma, pathologists have tried to establish a more detailed differentiation score. Our results confirm the findings in other ultrastructural (Balsaver et al, 1968;Ito and Johnson, 1969;Tremblay, 1971 ;Rousseau andNabarra, 1974), histoenzymatic (Jmbert andNezelof, 1972) and isoenzymatic (Cottreau et al, 1982) studies as well as the results obtained with an indirect immunofluorescence test using xenogeneic Wilms' antiserum (Kumar et al, 1980) which demonstrated the limited differentiation level of nephroblastoma. BGA expression displays the well differentiated tumour structures which are comparable to the degree of maturation of a 14 to 20-weekold fetal kidney.…”

Section: Discussionsupporting

confidence: 91%

Blood Group Antigen Expression in Nephroblastoma Department of Paediatric Pathology, Hôpital Necker Enfants Malades, Paris, France

Gang

Roth

Rousseau-Merck

et al. 1984

British Journal of Urology

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The histological distribution of A, B, H, Lewis, Pr, i and I blood group antigens (BGA) was studied in 19 nephroblastomas and one mesoblastic nephroma by means of the indirect immunofluorescence technique. In addition to general conclusions about the relatively limited BGA expression in the tumour cells, their histological distribution was found to be related to the differentiation level of these renal embryonic tumours. By comparison with normal embryonic, fetal and adult kidney it was shown that the nephroblastoma differentiation level is not higher than that of a 3-month fetal kidney. Furthermore, i antigen appears to be a non-specific marker of the histiocytic macrophage cells of the nephroblastoma stroma.

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Section: Discussionsupporting

confidence: 91%

Blood Group Antigen Expression in Nephroblastoma Department of Paediatric Pathology, Hôpital Necker Enfants Malades, Paris, France

Gang

Roth

Rousseau-Merck

et al. 1984

British Journal of Urology

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“…Although the rat L-isozyme has a PI value in the pH 5 range, it is unlikely that this form is related to the low PI variant expressed in the undifferentiated astrocyte. Similar low PI variants were observed in previous isoelectrofocusing studies of developing or neoplastic, muscle or brain tissues and immunological criteria suggest that these belong to the K-M isozyme family (Marie et al, 1976;Guguen-Guillouzo et al, 1977;Cottreau et al, 1982). The observed sensitivity of these low PI variants to heat denaturation and to alanine inhibition (see Results) suggests they are functionally related to the Kisozyme.…”

Section: Pyruvate Kinase Isozyme Expression In Differentiating Astrocsupporting

confidence: 83%

“…This latter conclusion is supported by isoelectrozymograms using pH 6-9 gradients that show that this band can be resolved into three under the proper conditions (data not shown). Such multiple bands between the K4-and M4-bands have also been observed by others (Ibsen et al, 1976, Guguen-Guillouzo et al, 1977Cottreau et al, 1982). Extracts of adult spleen yield a single peak with a PI value of 6.8, suggesting this variant is the K4-isozyme.…”

Section: Differentiation Of Pyruvate Kinase In Cultured Astrocytessupporting

confidence: 80%

Expression of pyruvate kinase in astrocytes induced to differentiate in vitro

Ngo

Ibsen

1989

J of Neuroscience Research

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Astrocytes maintained in a chemically defined media undergo differentiation and a parallel increase in pyruvate kinase specific activity. These changes are accompanied by a shift in the isoelectrofocusing pattern, but not by expression of pyruvate kinase M4, the characteristic adult rat brain isozyme. Thus, this chemically defined media lacks a substance required to induce pyruvate kinase M synthesis and this function can be uncoupled from other aspects of cellular differentiation. The uncoupling of pyruvate kinase maturation from cellular differentiation and the observation of only a single, 2.3 kilobase, pyruvate kinase mRNA molecule at different stages of the postnatal development of rat brain support the concept that the K- to M-isoform transformation is a post-transcriptional event. The effect of the individual components of this chemically defined medium on pyruvate kinase specific activity was studied by eliminating one component at a time. The increase in activity was found to be completely dependent upon fibroblastic growth factor and prostaglandin F2 alpha and was partially dependent on the simultaneous presence of insulin.

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“…Although this represents only a fraction of the total number of proteins in the nucleus, they constitute a broad spectrum of protein functions residing in the NB nucleus. The nuclear proteins identified associated pathologically with NB include GAPDH [28], pyruvate kinase [29], cofilin [30], nm23 [20], grp94 [23], SOD [24], stathmin [27], a-tropomyosin [31], and MIF [32]. In addition, in searching for potential biomarkers from the NB nucleus, we identified proteins (SET [15], transgelin 2 [16 -18], ubiquitin coenzyme E2A [21], eIF5A [33], EF 1 a-1 [34]) with correlations to other malignancies.…”

Section: Discussionmentioning

confidence: 99%