Pyruvate as a cell‐protective agent during cold atmospheric plasma treatment in vitro: Impact on basic research for selective killing of tumor cells

Bergemann, Claudia; Rebl, Henrike; Otto, Anik; Matschke, Svenja; Nebe, Barbara

doi:10.1002/ppap.201900088

Cited by 11 publications

(16 citation statements)

References 72 publications

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“…This could be explained by the very low concentration of H 2 O 2 observed in the treated medium (< 1 µM). H 2 O 2 is likely rapidly scavenged by pyruvate in the culture medium [44], [45], [46], disabling it from having any long-term effect on the cells.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Three Radio-Frequency Discharge Modes on the Treatment of Breast Cancer Cells in Vitro

Boisvert

Lafontaine²,

Glory³

et al. 2020

IEEE Trans. Radiat. Plasma Med. Sci.

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Non-thermal plasmas (NTPs) are known for their ability to induce thermal-free cytotoxic effects on cancer cells. However, as the variety of NTP devices increases, comparison of their cytotoxic effect becomes increasingly essential. In this work, we compare the cytotoxicity of three different radio-frequency NTPs. MDA-MB-231 triple negative breast cancer cells are treated in suspension in DMEM culture medium by the effluents of a single radio-frequency (RF) discharge device operating in three modes, namely the Ω and γ modes of the capacitively coupled radio-frequency (CCRF) discharge and a RF plasma jet mode. All three discharge modes reduce the proliferative capacity of MDA-MB-231 cells, but the treatment time required to reach the same efficacy is more than ten times longer using the Ω and the γ modes than using the jet mode. In all cases, using the appropriate treatment time, cells exhibit an impaired proliferation and eventually start to show signs of cell death (about 48 h after treatment). The three discharge modes also induce nuclear DNA damages. Plasma-produced H 2 O 2 was not found to contribute to the cytotoxicity of the treatment. Furthermore, short-lived reactive species (liquid phase species with a lifetime below 1 s) are expected to play an important role in the anti-cancer effect of all three discharge modes.

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Section: Discussionmentioning

confidence: 99%

Comparison of Three Radio-Frequency Discharge Modes on the Treatment of Breast Cancer Cells in Vitro

Boisvert

Lafontaine²,

Glory³

et al. 2020

IEEE Trans. Radiat. Plasma Med. Sci.

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“…Consequently, all cell lines were treated in the same culture medium (DMEM with pyruvate) to avoid this bias. DMEM with pyruvate was chosen to highlight the influence of direct NTP treatment over the capacity of plasma to produce long-term RONS such as H 2 O 2 [66][67][68][69].…”

Section: Conflicts Of Interestmentioning

confidence: 99%

Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines

Lafontaine¹,

Boisvert

Glory³

et al. 2020

Cancers

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Cancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, and can induce cancer cell death both in vitro and in vivo. Here, we used proliferation assay to characterize the plasma sensitivity of fourteen breast cancer cell lines. These assays showed that all tested cell lines were sensitive to NTP. In addition, a good correlation was found comparing cell sensitivity to NTP and radiation therapy (RT), where cells that were sensitive to RT were also sensitive to plasma. Moreover, in some breast cancer cell lines, NTP and RT have a synergistic effect. Adding a dose of PARP-inhibitor olaparib to NTP treatment always increases the efficacy of the treatment. Olaparib also exhibits a synergistic effect with NTP, especially in triple negative breast cancer cells. Results presented here help elucidate the position of plasma use as a potential breast cancer treatment.

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“…We incubated HaCaT cells in DMEM supplemented with 150 mg/L H 2 O 2 . We chose this concentration because, in previous work, we found an H 2 O 2 content of 120 mg/L for DMEM without pyruvate directly after CAP exposure and could show that the H 2 O 2 was depleted immediately by pyruvate in DMEM with 1 mM pyruvate [80]. In consideration of other anti-oxidative components contained in DMEM, such as Fe 3+ , which leads to the decomposition of H 2 O 2 by the Fenton reaction, we used the concentration of 150 mg/L.…”

Section: Discussionmentioning

confidence: 99%

“…The distance between the tip of the quartz capillary and the 96-well plate did not exceed 1 mm. In this way, the argon plasma had immediate contact with the media [45,80]. Argon gas treatment using the kINPen ® 09 without igniting the plasma for 60 s was used as a control (argon-activated medium, AAM).…”

Section: Plasma Treatment Of Cell Culture Mediamentioning

confidence: 99%

“…Furthermore, HaCaT cells were incubated in DMEM supplemented with hydrogen peroxide (H 2 O 2 ) to test the effect of a relatively long-lived reactive species, which usually arises from the interaction of CAP with a liquid medium. We used a concentration of 150 mg/L H 2 O 2 (from commercial stock solution, 30%, Fisher Scientific) in DMEM corresponding to the concentrations measured in an earlier study of our group [80].…”

Section: Plasma Treatment Of Cell Culture Mediamentioning

confidence: 99%

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The Hyaluronan Pericellular Coat and Cold Atmospheric Plasma Treatment of Cells

et al. 2020

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In different tumors, high amounts of hyaluronan (HA) are correlated with tumor progression. Therefore, new tumor therapy strategies are targeting HA production and degradation. In plasma medicine research, antiproliferative and apoptosis-inducing effects on tumor cells were observed using cold atmospheric plasma (CAP) or plasma-activated media (PAM). Until now, the influence of PAM on the HA pericellular coat has not been the focus of research. PAM was generated by argon-plasma treatment of Dulbecco’s modified Eagle’s Medium via the kINPen®09 plasma jet. The HA expression on PAM-treated HaCaT cells was determined by flow cytometry and confocal laser scanning microscopy. Changes in the adhesion behavior of vital cells in PAM were observed by impedance measurement using the xCELLigence system. We found that PAM treatment impaired the HA pericellular coat of HaCaT cells. The time-dependent adhesion was impressively diminished. However, a disturbed HA coat alone was not the reason for the inhibition of cell adhesion because cells enzymatically treated with HAdase did not lose their adhesion capacity completely. Here, we showed for the first time that the plasma-activated medium (PAM) was able to influence the HA pericellular coat.

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Pyruvate as a cell‐protective agent during cold atmospheric plasma treatment in vitro: Impact on basic research for selective killing of tumor cells

Cited by 11 publications

References 72 publications

Comparison of Three Radio-Frequency Discharge Modes on the Treatment of Breast Cancer Cells in Vitro

Comparison of Three Radio-Frequency Discharge Modes on the Treatment of Breast Cancer Cells in Vitro

Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines

The Hyaluronan Pericellular Coat and Cold Atmospheric Plasma Treatment of Cells

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