Pyrosequencing reveals the inhibitory impact of chronic exposure to erythromycin on activated sludge bacterial community structure

Pala‐Özkök, İlke; Rehman, Ateequr; Ubay‐Cokgor, Emine; Jonas, Daniel E; Orhon, Derin

doi:10.1016/j.bej.2014.06.003

Cited by 27 publications

(11 citation statements)

References 49 publications

(69 reference statements)

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“…During this period, a maximum storage rate, k STO , of 5.7 day −1 and the maximum rate for growth on storage,

{\overset{μ}{true}}_{italicSTO}

value of 3.8 were obtained. The overall process kinetics were quite compatible with the literature values for peptone mixture and domestic sewage . The model simulation of the experimental data provides rigorous proof for the validity and reliability of corresponding biodegradation characteristics assessed by means of model calibration.…”

Section: Resultssupporting

confidence: 76%

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Respirometric evaluation and modeling of the impact of continuous benzo[a]anthracene feeding on activated sludge

Başak

Güven

Çokgör

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND: This study evaluated the impact of continuous benzo[a]anthracene (B[a]A) feeding on the biodegradation of peptone/meat extract mixture by means of respirometric testing and modeling results, and microbial community analyses.A sequencing batch reactor (SBR) was operated at a sludge age of 10 days. At steady state, it was sustained with the peptone mixture feeding alone for 21 days. The following 21 days involved continuous dosing of 11 mg L −1 of B[a]A together with peptone mixture. In the last period of 7 days, B[a]A feeding was stopped. Storage of poly-hydroxy alkanoates (PHA) occurred during substrate utilization. A compatible model was calibrated using the oxygen uptake rate (OUR) and PHA profiles characterizing each phase. RESULTS: Continuous exposure to B[a]A did not affect the microbial growth process, but slightly increased PHA storage rate and growth on stored PHA. Hydrolysis rates were increased at first exposure followed by a significant decrease. Substrate binding also occurred at first exposure and declined by the end of B[a]A feeding. A major change was observed in biomass composition due to continuous exposure to B[a]A. CONCLUSION: The major novelty of the study was to prove that B[a]A altered the composition of the enriched microbial culture, characterized by slightly different process kinetics, especially for hydrolysable COD fractions.

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“…During this period, a maximum storage rate, k STO , of 5.7 day −1 and the maximum rate for growth on storage,

{\overset{μ}{true}}_{italicSTO}

Section: Resultssupporting

confidence: 76%

“…Modeling alone does not answer these questions, because a single active biomass parameter, X H presumably defines the entire heterotrophic population. Therefore, the recent trend considers – as in this study – microbial community analysis as an integral component of evaluations of process kinetics …”

Section: Resultsmentioning

confidence: 99%

Respirometric evaluation and modeling of the impact of continuous benzo[a]anthracene feeding on activated sludge

Başak

Güven

Çokgör

et al. 2019

J of Chemical Tech & Biotech

Self Cite

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“…Recently, Zhang et al [ 46 ] reported that Actinobacteria abundance increased in IBP-enriched planed beds compared to the control. Pala-Ozkok et al [ 47 ] also reported that the continuous exposure of activated sludge to erythromycin for three days caused preferential growth and predominance of members of Actinobacteria (increased from 48% to 22%). Actinobacteria could hydroxylate DCF to different products referring to the activity of a cytochrome P450 enzyme [ 48 ]…”

Section: Resultsmentioning

confidence: 99%

Impact of selected non-steroidal anti-inflammatory pharmaceuticals on microbial community assembly and activity in sequencing batch reactors

Jiang

Geng

et al. 2017

PLoS ONE

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This study covers three widely detected non-steroidal anti-inflammatory pharmaceuticals (NSAIDs), diclofenac (DCF), ibuprofen (IBP) and naproxen (NPX), as NSAIDs pollutants. The objective is to evaluate the impact of NSAIDs at their environmental concentrations on microbial community assembly and activity. The exposure experiments were conducted under three conditions (5 μg L-1 DCF, 5 μg L-1 DCF+5 μg L-1 IBP and 5 μg L-1 DCF+5 μg L-1 IBP+ 5 μg L-1 NPX) in sequencing batch reactors (SBRs) for 130 days. Removals of COD and NH4+-N were not affected but total nitrogen (TN) removal decreased. IBP and NPX had the high removal efficiencies (79.96% to 85.64%), whereas DCF was more persistent (57.24% to 64.12%). In addition, the decreased removals of TN remained the same under the three conditions (p > 0.05). The results of oxidizing enzyme activities, live cell percentages and extracellular polymeric substances (EPS) indicated that NSAIDs damaged the cell walls or microorganisms and the mixtures of the three NSAIDs increased the toxicity. The increased Shannon-Wiener diversity index suggested that bacterial diversity was increased with the addition of selected NSAIDs. Bacterial ribosomal RNA small subunit (16S) gene sequencing results indicated that Actinobacteria and Bacteroidetes were enriched, while Micropruina and Nakamurella decreased with the addition of NSAIDs. The enrichment of Actinobacteria and Bacteroidetes indicated that both of them might have the ability to degrade NSAIDs and thereby could adapt well with the presence of NSAIDs.

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“…Some bacteria secrete antibiotics and acids to inhibit other species, which may be destructive for maintaining a stable and effective bacterial community . To understand if any strains in this research could potentially inhibit others, cells of all well‐grown strains (sewage A 600 > 0.5 after cultivation for a week) were collected and pairs of strains were mixed equally and cultured in sewage for a week at 25°C in flasks.…”

Section: Methodsmentioning

confidence: 99%

Chemical Oxygen Demand Removal in Na₂SO₄ Saturated Wastewater by Cultivable Halotolerant Bacterial Community

Zhang

Pan

Hao

et al. 2016

CLEAN Soil Air Water

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Industrial wastewater with a high Na 2 SO 4 concentration is characterized by high osmotic pressure and salinity, and requires pretreatment before discharging into central treatment facilities. To date, few studies have addressed the chemical oxygen demand (COD) removal in biotreatment of Na 2 SO 4 wastewater. Here, a novel aerobic system for treating Na 2 SO 4 wastewater was developed based on screening halotolerant bacterial strains. The system maintained a stable COD removal at >90% in saturated concentration (varying from 5 to 40% depending on the temperature) of Na 2 SO 4 for 5 years. Activated sludge was initially constructed by cultured strains of Hyphomicrobium sp. CB03, Dietzia sp. XM15, Staphylococcus sp. T211, Flavobacterium sp. AXY1, Ochrobactrum sp. BY4, Bacillus sp. BYXT, Sphingobacterium sp.YY1, Rhodococcus sp. NH7-4, Stappia sp. HM4, Microbacterium sp. PY3-1, and Pseudomonas sp. MN3-2. Uncultured phylum TM7 was detected in the sludge after acclimatization. The present aerobic cultivable halotolerant bacterial community was successfully applied to large scale removal of organic pollutants in saline wastewater.Abbreviations: BOD 5 , biochemical oxygen demand after five days; CHBC, cultivable halotolerant bacterial community; COD, chemical oxygen demand; DO, dissolved oxygen; HRT, hydraulic retention time; MLSS, mixed liquor suspended solid; SEM, scanning electron microscopy 593

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Pyrosequencing reveals the inhibitory impact of chronic exposure to erythromycin on activated sludge bacterial community structure

Cited by 27 publications

References 49 publications

Respirometric evaluation and modeling of the impact of continuous benzo[a]anthracene feeding on activated sludge

Respirometric evaluation and modeling of the impact of continuous benzo[a]anthracene feeding on activated sludge

Impact of selected non-steroidal anti-inflammatory pharmaceuticals on microbial community assembly and activity in sequencing batch reactors

Chemical Oxygen Demand Removal in Na₂SO₄ Saturated Wastewater by Cultivable Halotolerant Bacterial Community

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Pyrosequencing reveals the inhibitory impact of chronic exposure to erythromycin on activated sludge bacterial community structure

Cited by 27 publications

References 49 publications

Respirometric evaluation and modeling of the impact of continuous benzo[a]anthracene feeding on activated sludge

Respirometric evaluation and modeling of the impact of continuous benzo[a]anthracene feeding on activated sludge

Impact of selected non-steroidal anti-inflammatory pharmaceuticals on microbial community assembly and activity in sequencing batch reactors

Chemical Oxygen Demand Removal in Na2SO4 Saturated Wastewater by Cultivable Halotolerant Bacterial Community

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Product

Resources

About

Chemical Oxygen Demand Removal in Na₂SO₄ Saturated Wastewater by Cultivable Halotolerant Bacterial Community