Pyrosequencing Reveals Fungal Communities in the Rhizosphere of Xinjiang Jujube

Liu, Peng; Wang, Xiaohui; Li, Jiangui; Qin, Wei; Xiao, Chengze; Zhao, Xu; Jiang, Hongxia; Sui, Junkang; Sa, Rachina; Wang, Wei-Yan; Liu, Xunli

doi:10.1155/2015/972481

Cited by 23 publications

(18 citation statements)

References 36 publications

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“…Previous studies have indicated the dominance of Ascomycota in truffle grounds (Mello et al, 2011) and in the rhizosphere of Xinjiang Jujube (Liu et al, 2015). Zygomycota was the second dominant phylum, which has also been reported as sub-dominant species in the Negev desert (Bates et al, 2010).…”

Section: Discussionmentioning

confidence: 89%

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

Al-Sadi¹,

Al-Khatri

Nasehi

et al. 2017

IJAB

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The study analyzed fungal diversity in soil trapped behind dams in three arid regions of Oman, in the south-eastern part of the Arabian Peninsula. Physicochemical analysis indicated that soils are of the loamy sand or sandy loam types and they are deficit in some macro and micro elements. Culture-based techniques revealed variation between dam soils in the number and type of phyla, classes, orders, genera and species. Ascomycota was the most dominant phyla in the three samples, contributing to 89% of the total species. Eurotiomycetes, Sordariomycetes and Dothideomycetes were the most common classes in dam soils. Zygomycetes, Pezizomycetes and Oomycetes were also detected, but at lower levels. Fifty fungal taxa were recovered from the three dam soils, with Aspergillus, Penicillium and Trichoderma being the most common genera. Most of the detected species were saprophytes; however some were either plant pathogens or mycotoxin producers, affecting humans directly or indirectly. This study appears to report for the first time 20 fungal species in Oman. It is also the first study from arid regions to characterize high fungal diversity in soil trapped behind dams. The sources of these fungi in dam soils and the implications of fungal diversity are discussed.

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Section: Discussionmentioning

confidence: 89%

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

Al-Sadi¹,

Al-Khatri

Nasehi

et al. 2017

IJAB

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“…Therefore, the sequencing capability was sufficiently large to capture the complete diversity of these communities. The Chao and ACE values are indicators of species richness, and Shannon and Simpson indices are indicators of species diversity (Liu et al 2015). The bacterial community richness and diversity indices were calculated (Table 4); no significant differences observed between the two soil samples.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Wang

Sui

et al. 2018

AMB Expr

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Rhizospheric microorganisms can increase phosphorus availability in the soil. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphate-solubilizing bacteria. The aim of the current study was to identify efficient phosphofungi that could be developed as commercial microbial agents. Among several phosphate-solubilizing fungal isolates screened, strain CS-1 showed the highest phosphorus-solubilization ability. Based on phylogenetic analysis of the internal transcribed spacer region sequence, it was identified as Aspergillus niger. High-performance liquid chromatography analysis revealed that the mechanism of phosphorus solubilization by CS-1 involved the synthesis and secretion of organic acids, mainly oxalic, tartaric, and citric acids. Furthermore, strain CS-1 exhibited other growth-promoting abilities, including efficient potassium release and degradation of crop straw cellulose. These properties help to returning crop residues to the soil, thereby increasing nutrient availability and sustaining organic matter concentration therein. A pot experiment revealed that CS-1 apparently increased the assessed biometric parameters of wheat seedlings, implying the potential of this strain to be developed as a commercial microbial agent. We used Illumina MiSeq sequencing to investigate the microbial community composition in the rhizosphere of uninoculated wheat plants and wheat plants inoculated with the CS-1 strain to obtain insight into the effect of the CS-1 strain inoculation. The data clearly demonstrated that CS-1 significantly reduced the content of pathogenic fungi, including Gibberella, Fusarium, Monographella, Bipolaris, and Volutella, which cause soil-borne diseases in various crops. Strain CS-1 may hence be developed into a microbial agent for plant growth improvement.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0593-4) contains supplementary material, which is available to authorized users.

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“…Presence of unidentified sequences signify their absence in cluster analysis databases, hence the need for research to identify more environmental fungal populations and collection of curated sequences for fungal identification (Liu et al 2015). The use of RHRW with human pathogenic isolates for domestic use and irrigation purposes without any prior treatment could therefore be a cause of concern if pathogens persist up to the point of consumption.…”

Section: Discussionmentioning

confidence: 99%

“…Despite being an expensive technique, the 454 pyrosequencing technique is rapid and flexible with 99% accuracy (Liu et al 2015). Therefore, this study aims to assess the microbiological quality of RHRW for irrigation and domestic purposes using culturable and non-culturable approaches for bacteria and fungi, respectively.…”

Section: Stated Thatmentioning

confidence: 99%

Microbial quality and suitability of roof-harvested rainwater in rural villages for crop irrigation and domestic use

Jongman

Korsten

2016

Journal of Water and Health

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The study aimed at assessing the microbiological quality and suitability of roof-harvested rainwater (RHRW) for crop irrigation and domestic use. In total, 80 rain water tanks (246 samples) across three rural villages (Ga-Molepane, Jericho and Luthngele) were visited. Culture based techniques were used to isolate bacterial microbes and identities were confirmed using matrix assisted laser desorption/ ionization time of flight (MALDITOF MS). Uncultured fungal populations were also identified using pyrosequencing. Salmonella spp. (3%), L. monocytogenes (22%), total coliforms (57.7%), Escherichia coli (E. coli) (30.5%), Enterococcus spp. (48.8%), Pseudomonas spp. (21.5%) were detected in RHRW samples after rainfall. Fungal sequences belonging to species known to cause fever, coughing and shortness of breath in humans (Cryptococcus spp.) were identified. This study indicates that RHRW quality can be affected by external factors such as faecal material and debris on the rooftops. The use of untreated RHRW could pose a potential health risk if used for irrigation of crops or domestic use, especially in the case of a relative high population of immunocompromised individuals. This study does not dispute the fact that RHRW is an alternative irrigation water source but it recommends treatment before use for domestic purposes or for watering crops.

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Pyrosequencing Reveals Fungal Communities in the Rhizosphere of Xinjiang Jujube

Cited by 23 publications

References 36 publications

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

High Fungal Diversity and Dominance by Ascomycota in Dam Reservoir Soils of Arid Climates

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Microbial quality and suitability of roof-harvested rainwater in rural villages for crop irrigation and domestic use

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