Pyrosequencing-based assessment of soil microbial community structure and analysis of soil properties with vegetable planted at different years under greenhouse conditions

Jian, Zhang; Wang, Pengcheng; Tian, Hongmei; Xiao, Qing; Jiang, Haikun

doi:10.1016/j.still.2018.11.008

Cited by 41 publications

(18 citation statements)

References 54 publications

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“…Species from this phylum are considered to be the primary functional bacteria for the decomposition and transformation of litter, which aligns with previous research results [39,40]. However, the relative abundance of Proteobacteria in ZM was lower than that in forested land, which was consistent with results showing that the relative abundance of Proteobacteria increased significantly with higher organic matter content [41,42].…”

Section: Discussionsupporting

confidence: 91%

Bacterial Community Changes Associated with Land Use Type in the Forest Montane Region of Northeast China

Deng

Yin

et al. 2019

Forests

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Soil microorganisms play a vital role in the biogeochemical cycle, whereas land use change is one of the primary factors that affects the biodiversity and functionality of terrestrial ecosystems. The composition and diversity of bacterial communities (by high-throughput sequencing of the bacterial 16S rRNA gene) were evaluated in the soils of the Montane Region of Northeast China, across different land use types, e.g., natural secondary forest (Quercus mongolica, QM), shrubland (SL), coniferous plantation (Larix gmelinii, LG, and Pinus koraiensis, PK), and agricultural land (Zea mays, ZM). Significant differences in the chemical characteristics and bacterial communities in soils under different land uses were observed in this study. Soil resident TC (total carbon) and TN (total nitrogen) were much higher in secondary natural forest soils, than in coniferous plantation and agricultural soils. Compared with forest and shrubland soils, soil bacterial OTUs, the Chao1 index, and the ACE index were the lowest in the ZM. There were high proportions of Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, Gemmatimonadetes, Verrucomicrobia, Bacteroidetes, Planctomycetes, Saccharibacteria, and Nitrospirae in agricultural and forest soils, which accounted for over 90% of the reads in each sample. We found that the dominant group in the forest and shrubland soils was Proteobacteria, while the most dominant group in the ZM was Actinobacteria. The results of both heatmap and principal component analyses displayed groups according to land use types, which indicated that the bacterial communities in the areas under study were significantly influenced by long term differently managed land use. Furthermore, redundancy and Pearson correlation analyses revealed that the bacterial communities were primarily regulated by soil characteristics. This suggested that altered land use patterns initiated changes in the chemical properties of the soils, which affected the composition of microbial communities in this area. This provides a scientific basis for the evolutionary mechanism of soil quality, as well as the rational development and utilization of land resources.

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Section: Discussionsupporting

confidence: 91%

Bacterial Community Changes Associated with Land Use Type in the Forest Montane Region of Northeast China

Deng

Yin

et al. 2019

Forests

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“…Similarly, AP was 1.3 and 10.1 times higher in Shenyang (Meng et al, 2000), but 1.09 and 6.39 times in Lhasa. The SOM value was also 3.56 times higher than the control in in Anhui Province (Zhang et al, 2019) as it was 0.87 and 1.65 times higher in Lhasa. These results show that the degree of change in greenhouse soil fertility in Lhasa remained relatively minor compared to that in the eastern China.…”

Section: A Regional Comparison Of Greenhouse Soil Fertility Changesmentioning

confidence: 72%

“…Soil changes affect the substrate (Zhang et al, 2019) as well as crop yield and quality (Chen et al, 2017) and can also cause environmental problems, such as water pollution (He, 2006;Heredia and Cirelli, 2007;Ju et al, 2007;Kalkhajeh et al, 2018) and ecological destruction. Earlier study has shown that when soil Olsen-P exceeds 60 mg/kg, P in drainage water increases to more than 2 mg/L (Heckrath et al, 1995).…”

Section: Soil Change Strategies In Lhasamentioning

confidence: 99%

Investigating the Effects of Greenhouse Vegetable Cultivation on Soil Fertility in Lhasa, Tibetan Plateau

2020

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The area of land utilized for growing vegetables in greenhouses has expanded rapidly on the Tibetan Plateau over recent decades. However, the effects of greenhouses on soil fertility as well as variations in these effects between the plateau and plain remain unclear on the Tibetan Plateau. This study assessed the effects of vegetable greenhouses in the vicinity of Lhasa, using open field soil as a control. A total of 92 plough layer (0−20 cm depth) soil samples including 54 from greenhouses and 38 from open fields were taken, and soil pH, electrical conductivity (EC), total soluble salt (TS), soil organic matter (SOM), total nitrogen (TN), available phosphorus (AP), and available potassium (AK) were measured. The results reveal that, soil pH was lower 1.0 units in greenhouses than that in open field. TS was higher 82% and AP was higher 160% overall. Similarly, SOM and TN were higher 32% and 46%, respectively, while AK changed slightly at a higher 1% rate. Results also show that soil properties varied depending on cultivation time and vegetable types. Overall, pH continuously decreased with cultivation time while other soil fertility indicators reached a maximum value after nine years of cultivation before starting to decrease. The effect of leafy vegetable planting on soil was slight overall, while the impact of fruits on soil was more serious. Compared with changes in plain greenhouse soil fertility measured across the eastern China, the effects of greenhouses on soil in Lhasa remain relatively limited; and the change in the degree of soil fertility was lower and the extreme values of soil fertility occurred later in Lhasa.

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“…Soil pH and OM content were detected following our previously reported methods (Zhang et al, 2019). The total N (TN) and alkaline N (AN) of the soil and the N content of the plant were determined following a reported method (Bremner, 1960).…”

Section: Determination Of Soil Propertiesmentioning

confidence: 99%

“…Available P (AP) was extracted and detected following the procedures of a reported method (Li et al, 2004). Soil available K (AK) and plant total K were measured using flame atomic absorption spectrophotometry following a previous method (Zhang et al, 2019).…”

Section: Determination Of Soil Propertiesmentioning

confidence: 99%

Effect of no-tillage and tillage systems on melon (Cucumis melo L.) yield, nutrient uptake and microbial community structures in greenhouse soils

Zhang

Wang

et al. 2020

Folia Horticulturae

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No-tillage (UT) and tillage (TL) influence melon (Cucumis melo L.) production. However, the mechanism of improving the soil quality under UT in melon production is still unavailable. In this study, we attempted to explore the effects of UT and TL treatments on soil fertility and the microbial abundance and diversity in planting melon under greenhouse condition. Soil properties were determined and the bacterial v4-v5 16S rRNA and the fungal internal transcribed spacer gene were pyrosequenced by extracting greenhouse soil DNA. Results showed that the two treatments had different effects on nutrient uptake in melon plants under facility conditions. Additional nitrogen (N) was absorbed in the leaves and fruit in UT treatment. However, the N content in the UT treatment was kept as similar to that of the TL treatment. The phosphorus (P) contents in melon plant leaves and fruits in the UT treatment were higher than those in the TL treatment. High potassium (K) contents were observed in fruits and melon stem under the UT and TL treatments, respectively. Soil pH, organic matter and the available N influenced the bacterial and fungal distributions. The total N, total P and total K in melon plants were correlated with the bacterial and fungal groups in facility soils. The UT treatment had a substantial effect on the microbial diversity in soils planted with melon. Our study provided insights into the response of soil fertility and microbial structures to UT and TL treatments under greenhouse soils, which may aid in managing greenhouse soil quality.

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Pyrosequencing-based assessment of soil microbial community structure and analysis of soil properties with vegetable planted at different years under greenhouse conditions

Cited by 41 publications

References 54 publications

Bacterial Community Changes Associated with Land Use Type in the Forest Montane Region of Northeast China

Bacterial Community Changes Associated with Land Use Type in the Forest Montane Region of Northeast China

Investigating the Effects of Greenhouse Vegetable Cultivation on Soil Fertility in Lhasa, Tibetan Plateau

Effect of no-tillage and tillage systems on melon (Cucumis melo L.) yield, nutrient uptake and microbial community structures in greenhouse soils

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