PYROPHOSPHATASE ACTIVITY OF
            <i>BACILLUS MEGATERIUM</i>
            SPORE AND VEGETATIVE CELL EXTRACTS

Levinson, Hillel S.; Sloan, James D.; Hyatt, Mildred T.

doi:10.1128/jb.75.3.291-299.1958

Cited by 23 publications

(13 citation statements)

References 32 publications

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“…Cali, Colombia. and spore activities of B. mwegateriiun?i could be found. In confirmation of the findings of Levinson et al (7), Mn++ was observed to have an important influence on the stability and structure of this enzyme. Glucose-6-phosphate anid a-glycerophosphate were obtained from Sigma Chemical Co., St. Louis, Mo., adenosine monophosphate from Pabst Laboratories, lysozyme from Calbiochem, Los Angeles, Calif., and streptomycin sulfate from Merck Chemical Div., RahwaY, N.J.…”

supporting

confidence: 89%

Biochemical Studies of Bacterial Sporulation IV. Inorganic Pyrophosphatase of Vegetative Cells and Spores of Bacillus megaterium

Tono

Kornberg

1967

J Bacteriol

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Inorganic pyrophosphatase activities extracted from vegetative cells and spores ol Bacillius Imnegateriulmz were compared and found to be similar in behavior on polyacrylamide gel electrophoresis, in metal and pH requirements for activity, and in response to inhibitors. In the sporulating cell, an additional electrophoretic species of the enzyme was observed which could be partially converted to the principal form by treatment witlh Mn++; both forms of the enzyme required Mn++ for stabilization in solution as well as for activity. Inorganic pyrophosphatases obtained in a near homogeneous state from vegetative cells and spores of Bacillus subtilis proved to be indistinguishable when judged by a number of physical and functional criteria (H. Tono and A. Kornberg, J. Biol. Chem., in press). Similar results were obtained in recent studies of deoxyribonucleic acid (DNA) polymerase (1) and adenylate kinase in B. subtilis (J. A. Spudich and A. Kornberg, unpublished data), or nucleoside phosphorylase in B. cereius (R. Gardner and A. Kornberg, J. Biol. Chem., in press), and in studies of other enzymes as reviewed by Halvorson (2). From these results we derive the impression that a given spore enzyme and its vegetative-cell counterpart are the same despite their markedly different resistance to heat when tested in situ. For the long-range objective of defining the components and organization of a spore and the mechanisms

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supporting

confidence: 89%

Biochemical Studies of Bacterial Sporulation IV. Inorganic Pyrophosphatase of Vegetative Cells and Spores of Bacillus megaterium

Tono

Kornberg

1967

J Bacteriol

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“…Such high levels of uptake suggest that manganese has an important function within spores. Many authors have explored the role of manganese ions in several of the spore's resistance properties (Ghosh et al, 2011;Levinson & Hyatt, 1964), as well as in germination (Levinson & Sevag, 1954;Levinson, Sloan, & Hyatt, 1958). However, only one group has reported the intrinsic magnetic properties conferred by the high manganese content in spores (Melnik et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Paramagnetism in Bacillus spores: Opportunities for novel biotechnological applications

Zhou

Ionescu

Wan

et al. 2017

Biotech & Bioengineering

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Spores of Bacillus megaterium, Bacillus cereus, and Bacillus subtilis were found to exhibit intrinsic paramagnetic properties as a result of the accumulation of manganese ions. All three Bacillus species displayed strong yet distinctive magnetic properties arising from differences in manganese quantity and valency. Manganese ions were found to accumulate both within the spore core as well as being associated with the surface of the spore. Bacillus megaterium spores accumulated up to 1 wt.% manganese (II) within, with a further 0.6 wt.% adsorbed onto the surface. At room temperature, Bacillus spores possess average magnetic susceptibilities in the range of 10−6 to 10−5. Three spore‐related biotechnological applications—magnetic sensing, magnetic separation and metal ion adsorption—were assessed subsequently, with the latter two considered as having the most potential for development.

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“…Activation of pyrophosphatase was not ob served in the presence of Ca^^, Cu^^, Al^*, and Fe^^ (Searle and Hughes, 1977). The results reported by Tono and Romberg (1967a) and Levinson et al (1958) for pyrophosphatase iso lated from Bacillus meqaterium contradict this information;…”

Section: Inorganic Pyrophosphatase Activity Of Soilsmentioning

confidence: 85%

“…coli by Josse and Wong (1971), B. megaterium by Levinson et al (1958) , Streptococcus faecalis by Oginski and Rumbaugh (1955) and Ferrobacillus ferrooxidans by Howard and Ludgren (197 0) , insects (Gilmour and Calaby, 1953;McElroy et al, 1951), mammalian tissues (Elliot, 1957;Naganna and Menon, 1948;Rafter, 1958), and plants (Fleury and Courtois, 1957;Naganna et al, 1955).…”

Section: Inorganic Pyrophosphatase Activity Of Soilsmentioning

confidence: 99%

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Phosphatases in the soil environment

Dick

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PYROPHOSPHATASE ACTIVITY OF BACILLUS MEGATERIUM SPORE AND VEGETATIVE CELL EXTRACTS

Abstract: We have long felt that a study aimed at correlating the manganese stimulation of germination and respiration of Bacillus megaterium spores (Levinson and Sevag, 1953) with Mn++

Cited by 23 publications

References 32 publications

Biochemical Studies of Bacterial Sporulation IV. Inorganic Pyrophosphatase of Vegetative Cells and Spores of Bacillus megaterium

Biochemical Studies of Bacterial Sporulation IV. Inorganic Pyrophosphatase of Vegetative Cells and Spores of Bacillus megaterium

Paramagnetism in Bacillus spores: Opportunities for novel biotechnological applications

Phosphatases in the soil environment

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