Pyropheophorbide-α methyl ester-mediated photodynamic therapy induces apoptosis and inhibits LPS-induced inflammation in RAW264.7 macrophages

Huang, Liyi; Chen, Qing; Yu, Lehua; Bai, Dingqun

doi:10.1016/j.pdpdt.2018.12.002

Cited by 28 publications

(21 citation statements)

References 35 publications

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“…Single-walled carbon nanotubes, another efficient photothermal ablation agent, were also found to upregulate Caspase-3 and induce apoptosis and thereby ablate vascular macrophages [ 27 ]. Pyropheophorbide-α methyl ester-mediated PDT has been shown to induce apoptosis in RAW264.7 cells via the activation of the mitochondrial caspase pathway [ 28 ]. However, the excessive apoptosis of plaque cells is an independent risk factor for AS and contributes to impaired phagocytic clearance of macrophages, resulting in a proinflammatory response and secondary necrosis.…”

Section: Resultsmentioning

confidence: 99%

Black TiO2 nanoprobe-mediated mild phototherapy reduces intracellular lipid levels in atherosclerotic foam cells via cholesterol regulation pathways instead of apoptosis

Dai

et al. 2022

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Black TiO2 nanoprobe-mediated mild phototherapy reduces intracellular lipid levels in atherosclerotic foam cells via cholesterol regulation pathways instead of apoptosis

Dai

et al. 2022

Bioactive Materials

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“…Since inflammatory processes are very prominent in a number of macrophage-related diseases, targeting macrophages may be an ideal strategy for treating inflammation and diseases. For example, for RA, LPS stimulation can directly promote the differentiation of macrophage RAW264.7 osteoclasts and inhibit osteoclast apoptosis, thereby aggravating inflammation and bone destruction ( Huang et al, 2019 ). Thus, FOXO1, FOXO2, and FOXO4 have also been detected in macrophage RAW264.7 cells, and the result showed that 18β-GA treatment increased the mRNA levels of FOXO1, FOXO2, and FOXO4 ( Figure 3D ).…”

Section: Discussionmentioning

confidence: 99%

“…Meanwhile, in this inflammatory microenvironment, synoviocytes will shift to a hyperproliferative state, which will then amplify the inflammatory response ( Mcinnes and Schett, 2011 ). Moreover, it has been demonstrated that the number and phenotype of macrophages will affect the development of disease ( Huang et al, 2019 ). Therefore, targeting synoviocytes and immune cells such as macrophages to interrupt the vicious circle has emerged as a potential treatment strategy for RA.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory and Pro-apoptotic Effects of 18beta-Glycyrrhetinic Acid In Vitro and In Vivo Models of Rheumatoid Arthritis

et al. 2021

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18β-Glycyrrhetinic acid (18β-GA), an active component from Glycyrrhiza glabra L. root (licorice), has been demonstrated to be able to protect against inflammatory response and reduce methotrexate (MTX)-derived toxicity. This study was therefore designed to test the therapeutic possibility of 18β-GA on rheumatoid arthritis (RA) and to explore the underlying mechanism. LPS or TNF-α-induced inflammatory cell models and collagen-induced arthritis (CIA) animal models were applied in this study. Real-time quantitative PCR (RT-qPCR) was used to measure the mRNA levels of various cytokines and FOXO family members. The protein levels of molecules in the MAPK/NF-κB signaling pathway were analyzed using western blot. The cell proliferation assay and colony-forming assay were used to test the influence of 18β-GA on cell viability. The cell apoptosis assay and cell cycle assay were performed to detect the effect of 18β-GA on cell proliferative capacity by using flow cytometry. Hematoxylin and eosin (H&E) staining was performed to evaluate pathological changes after drug administration. The enzyme-linked immunosorbent assay (ELISA) was carried out for the detection of cytokines in serum. In vitro, we found that 18β-GA decreased the mRNA levels of IL-1β, IL-6, and COX-2 by inhibiting the MAPK/NF-κB signaling pathway in MH7A and RAW264.7 cell lines. Moreover, 18β-GA was able to suppress cell viability, trigger cell apoptosis, and G1 phase cell cycle arrest in our in vitro studies. 18β-GA dramatically enhanced the mRNA level of FOXO3 in both TNF-α- and LPS-induced inflammation models in vitro. Interestingly, after analyzing GEO datasets, we found that the FOXO3 gene was significantly decreased in the RA synovial tissue as compared to healthy donors in multiple microarray studies. In vivo, 18β-GA exhibited a promising therapeutic effect in a collagen-induced arthritis mouse model by alleviating joint pathological changes and declining serum levels of TNF-α, IL-1β, and IL-6. Finally, we observed that 18β-GA administration could mitigate liver damage caused by collagen or MTX. Collectively, the current study demonstrates for the first time that 18β-GA can inhibit inflammation and proliferation of synovial cells, and the underlying mechanism may be associated with its inhibition of MAPK/NF-κB signaling and promotion of FOXO3 signaling. Therefore, 18β-GA is expected to be a new drug candidate for RA therapy.

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“…Besides its effects described here, it has additional antitumor effects including ablation and mechanical disruption of cancer tissue [28,29]. HIUS has already been shown to be useful in the treatment of uterine fibroids [30], various solid tumors of pancreas, liver, renal system, and prostate, and breast cancer [31][32][33][34]. So far, there have been no or few studies for potential use in peritoneal metastases (PM).…”

Section: Discussionmentioning

confidence: 99%

Increased Tissue Penetration of Doxorubicin in Pressurized Intraperitoneal Aerosol Chemotherapy (PIPAC) after High-Intensity Ultrasound (HIUS)

Khosrawipour

Reinhard

Martino

et al. 2019

International Journal of Surgical Oncology

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Background. High-intensity ultrasound (HIUS) has been studied for the past two decades as a new therapeutic option for solid tumor direct treatment and a method for better chemotherapy delivery and perfusion. is treatment approach has not been tested to our knowledge in peritoneal metastatic therapy, where limited tissue penetration of intraperitoneal chemotherapy has been a main problem. Both liquid instillations and pressurized aerosols are affected by this limitation. is study was performed to evaluate whether HIUS improves chemotherapy penetration rates. Methods. High-intensity ultrasound (HIUS) was applied for 0, 5, 30, 60, 120, and 300 seconds on the peritoneal tissue samples from fresh postmortem swine. Samples were then treated with doxorubicin via pressurized intraperitoneal aerosol chemotherapy (PIPAC) under 12 mmHg and 37°C temperature. Tissue penetration of doxorubicin was measured using fluorescence microscopy on frozen thin sections. Results. Macroscopic structural changes, identified by swelling of the superficial layer of the peritoneal surface, were observed after 120 seconds of HIUS. Maximum doxorubicin penetration was significantly higher in peritoneum treated with HIUS for 300 seconds, with a depth of 962.88 ± 161.4 μm (p < 0.05). Samples without HIUS had a penetration depth of 252.25 ± 60.41. Tissue penetration was significantly increased with longer HIUS duration, with up to 3.8-fold increased penetration after 300 sec of HIUS treatment. Conclusion. Our data indicate that HIUS may be used as a method to prepare the peritoneal tissue for intraperitoneal chemotherapy. Higher tissue penetration rates can be achieved without increasing chemotherapy concentrations and preventing structural damage to tissue using short time intervals. More studies need to be performed to analyze the effect of HIUS in combination with intraperitoneal chemotherapy.

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Pyropheophorbide-α methyl ester-mediated photodynamic therapy induces apoptosis and inhibits LPS-induced inflammation in RAW264.7 macrophages

Cited by 28 publications

References 35 publications

Black TiO2 nanoprobe-mediated mild phototherapy reduces intracellular lipid levels in atherosclerotic foam cells via cholesterol regulation pathways instead of apoptosis

Black TiO2 nanoprobe-mediated mild phototherapy reduces intracellular lipid levels in atherosclerotic foam cells via cholesterol regulation pathways instead of apoptosis

Anti-inflammatory and Pro-apoptotic Effects of 18beta-Glycyrrhetinic Acid In Vitro and In Vivo Models of Rheumatoid Arthritis

Increased Tissue Penetration of Doxorubicin in Pressurized Intraperitoneal Aerosol Chemotherapy (PIPAC) after High-Intensity Ultrasound (HIUS)

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