Pyrophen Produced by Endophytic Fungi Aspergillus sp Isolated from Piper crocatum Ruiz &amp; Pav Exhibits Cytotoxic Activity and Induces S Phase Arrest in T47D Breast Cancer Cells

Astuti, Puji; Erden, Willy; Wahyono, Wahyono; Wahyuono, Subagus; Hertiani, Triana

doi:10.7314/apjcp.2016.17.2.615

Cited by 11 publications

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“…Previous study reported that pyrophen isolated from endopytic fungi Aspergillus fumigatus KARSV04 showed toxicity towards p53 defective breast cancer cell T47D. 16 Further exploration on its potency to kill other breast cancer cell was examined by observing its toxicity towards p53 competent cells MCF-7 cells. In this study we found that pyrophen showed cytotoxity towards MCF-7.…”

Section: Discussionmentioning

confidence: 99%

“…Similar finding was found upon treating T47D with this compound alone. 16 On the other hand, it is indicated that pyrophen increased G2/M The difference response upon treatment of T47D and MCF-7 cells with pyrophen might be explained by the fact that these two breast cancer cell line have different characteristics in that T47D cells are p53 defective mutants whilst MCF-7 bearing p53 wildtype. 21 The accumulation of T47D cells in S phase may be in part due to inability of cells to promote p53 dependent cell death and rely on S phase arrest following treatment of pyrophen.…”

Section: Discussionmentioning

confidence: 99%

“…Pyrophen was isolated from the culture of endophytic fungi Aspergillus fumigatus strain KARSV04 (Culture collection of Pharmaceutical Biology Department, Faculty of Pharmacy UGM). 16 RPMI 1640, Fetal Bovine Serum, Penicillin -Streptomycin, Fungizon, Sodium bicarbonate, were supplied from Gibco. 4-(2hydroxyethyl)-1-piperazine ethane sulfonic acid (HEPES) were obtained from Invitrogen; Phosphate-Buffered Saline (PBS), propidium iodide (PI), Annexin -V-FLUOS staining kit, 3-(4, 5-dimethylthiazol-2-yl)-2, 5diphenyltetrazoliumbromide (MTT), RNAse, doxorubicin (Dox) were purchased from Sigma-Aldrich.…”

Section: Methodsmentioning

confidence: 99%

“…Previous study reported the cytotoxic effect in vitro of pyrophen on T47D breast cancer cells. 16 In order to assess the anti-cancer activity of this compound on other molecular subtype of breast cancer, MCF-7 cell line was used. MCF-7 cells were treated with pyrophen at the indicated concentrations of 11.25 -90.00 µg/mL, and the cell viability was determined by MTT assay.…”

Section: Pyrophen Modulates the Growth Of Mcf-7 Cellsmentioning

confidence: 99%

“…Previous finding demonstrated that pyrophen isolated from Aspergillus sp endophytic fungi modulated T47D cell cycle. 16 This study was aimed to examine whether pyrophen enhanced chemotherapeutic effect of doxorubicin in T47D as well as in other type of cells, MCF7.…”

Section: Introductionmentioning

confidence: 99%

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Pyrophen isolated from endophytic fungi Aspergillus fumigatus strain KARSV04 synergizes the effect of doxorubicin in killing MCF7 but not T47D cells

Astuti¹,

Januarti²,

Kiromah³

et al. 2020

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Introduction: Pyrophen, an amino acid-pyrone derivative isolated from Aspergillus fumigatus strain KARSV04 has been reported to exhibit anticancer effect on T47D cells by inhibiting the growth of the cells and modulate the cell cycle on S phase. Present study, effect of pyrophen in the doxorubicin (Dox) chemotherapy on an in vitro model of breast cancers was studied. Methods: The cytotoxicity of pyrophen, doxorubicin and in combination were evaluated in T47D and MCF-7 cells using MTT assay. Modulation on cell cycle distribution and apoptosis was examined by flow cytometry. Results: Our findings showed that pyrophen did not significantly potentiate Dox-induced cytotoxicity in T47D cells. Adding Dox treated T47D cells with pyrophen at concentration of 9.20 µg/mL induced slight increase of S-phase cell population. This compound induced cytotoxicity of MCF-7 cells with IC50 of 70.57 µg/mL. Co-treatment of pyrophen and Dox in MCF-7 cells increased cytotoxicity relative to Dox alone, which was suggested in part due to modulation of cell cycle at G2/M phase and apoptosis. Conclusion: The data suggest different mechanisms of regulation in promoting cell death by two different cell lines in response to administration of pyrophen.u n c o r r e c t e d p r o o f u n c o r r e c t e d p r o o f 2 ÖZAmaç: Aspergillus fumigatus KARSV04'ten izole edilen amino asit-piron türevi olan pirofen, hücrelerin büyümesini engeller ve S fazındaki hücre döngüsünü modüle ederek T47D hücreleri üzerinde antikanser etkisi gösterdiği rapor edilmiştir. Bu çalışmada, doksorubisin (Dox) kemoterapisindeki pirofen meme kanseri modelinde "in vitro" modeli üzerindeki etkisi incelenmiştir. Metotlar: Pirofen, doksorubisin ve kombinasyon halindeki sitotoksisite, MTT tahlili kullanılarak T47D ve MCF-7 hücrelerinde değerlendirildi. Hücre döngüsü dağılımı ve apoptozis modülasyonu akış sitometrisi ile incelendi. Bulgular: Bulgularımız, pirofen, T47D hücrelerinde Dox kaynaklı sitotoksisiteyi önemli ölçüde artırmadığını gösterdi. Pirofen eklenmesi, 9.20 µg/mL konsantrasyonunda Dox ile muamele edilmiş T47D hücrelerine S-faz hücre popülasyonunda hafif bir artışa neden oldu. Bu bileşik, IC50 70.57 µg/mL olan MCF-7 hücrelerinin sitotoksisitesini tesir etmistir. MCF-7 hücrelerinde pirofen ve Dox'un birlikte işlenmesi, sadece Dox ile karşılaştırıldığında sitotoksisiteyi arttırmıştır; bu, kısmen G2/M fazındaki hücre döngüsünün modülasyonu ve apoptoz nedeniyle önerilmiştir. Sonuç: Veriler, pirofen uygulamasına cevap olarak iki farklı hücre çizgisi tarafından hücre ölümünü teşvik etmede farklı düzenleme mekanizmaları olduğunu göstermektedir.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Pyrophen Modulates the Growth Of Mcf-7 Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pyrophen isolated from endophytic fungi Aspergillus fumigatus strain KARSV04 synergizes the effect of doxorubicin in killing MCF7 but not T47D cells

Astuti¹,

Januarti²,

Kiromah³

et al. 2020

tjps

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Nanochitosan derived from marine endolichenic fungi

Molehin¹,

Adeniyi²,

Olowomofe³

et al. 2023

Next Generation Nanochitosan

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Total Synthesis of Pyrophen and Campyrones A–C

Reber

Burdge

2018

J. Nat. Prod.

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The first total syntheses of the natural products pyrophen and campyrones A-C, isolated from the fungus Aspergillus niger, have been achieved in six steps starting from commercially available N-Boc amino acids. Key steps in this sequence include a vinylogous Claisen condensation to achieve fragment coupling and a dioxinone thermolysis/cyclization cascade to form the α-pyrone ring. The route described herein afforded the natural products in 15-25% overall yield, furnishing sufficient material for testing in biological assays.

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Pyrophen Produced by Endophytic Fungi Aspergillus sp Isolated from Piper crocatum Ruiz & Pav Exhibits Cytotoxic Activity and Induces S Phase Arrest in T47D Breast Cancer Cells

Cited by 11 publications

References 13 publications

Pyrophen isolated from endophytic fungi Aspergillus fumigatus strain KARSV04 synergizes the effect of doxorubicin in killing MCF7 but not T47D cells

Pyrophen isolated from endophytic fungi Aspergillus fumigatus strain KARSV04 synergizes the effect of doxorubicin in killing MCF7 but not T47D cells

Nanochitosan derived from marine endolichenic fungi

Total Synthesis of Pyrophen and Campyrones A–C

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