Pyrolysis mass spectra, sulfhydryl and tryptophan content of the embryonic nuclei from adult human normal and nuclear-cataractous lenses

Haard, P.M.M. van; Hoenders, Herman J.; Wollensak, J.; Haverkamp, Johan

doi:10.1016/0304-4165(80)90065-3

Cited by 20 publications

(2 citation statements)

References 21 publications

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“…It has to be mentioned that the theoretical specific absorbance at 280 nm due to 2 Trp residues and 5 or 6 Tyr residues [16] is always smaller than the experimental value [17]. In the present study it corresponds to 30% of the observed specific absorbance.…”

Section: Biochemical and Physicochemical Analysiscontrasting

confidence: 53%

Degradation of crystallins from a psoriatic patient undergoing PUVA therapy

Melo¹,

Castro

Ribeiro³

et al. 1990

FEBS Letters

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Comparative physico-chemical and spectroscopic analyses were carried out in human lens proteins obtained from extracts of normal, senile and PUVA cataracts. Mass recovery analysis reveals a large protein concentration loss in the PUVA cataract relative to the normal lens and senile cataract. This protein loss parallels an increase in the degraded polypeptide chains. However, the tryptophan content (2.1 mol/mol of 20 kDa protein subunit) and the apparent fluorescence quantum yield (cpr=O.O56) of the tryptophan residues which are believed to be involved in the development of UV-induced cataracts are unchanged after age-related alterations and/or in vivo photochemistry associated with psoralen @MOP) photosensitized reactions.

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Section: Biochemical and Physicochemical Analysiscontrasting

confidence: 53%

Degradation of crystallins from a psoriatic patient undergoing PUVA therapy

Melo¹,

Castro

Ribeiro³

et al. 1990

FEBS Letters

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“…The nucleus is defined as the hard inner part, obtained after peeling off the cortical layers of the lens. This part corres ponds to the embryonic lens [29], According to Van Haard et al [35], the embryonic nucleus in human cataractous lenses is constant in weight (30 ± 2 mg; 14% of wet weight). The nucleus has also been defined as a core of 80% of the lens diameter after dissecting the anterior and post erior parts, which in turn are called anterior and posterior cortices [17].…”

Section: The Lensmentioning

confidence: 99%

Guidelines for the Classification of Lenses and the Characterization of Lens Proteins

1982

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Determination of protein-bound methionine oxidationin the hippocampus of adult and old rats by LC-ESI-ITMS method after microwave-assisted proteolysis

Long

Guan

et al. 2011

Anal Bioanal Chem

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Protein-bound methionine (Met) oxidation has been associated with normal aging and a variety of age-related diseases, including Alzheimer's disease and Parkinson's disease. Monitoring the changes of protein-bound methionine content in the brain in response to normal aging and oxidative stress is of great interest and could be used as an indicator of oxidative stress of rats in pathological conditions. We have developed a rapid analytical method for the determination of oxidized products of protein-bound methionine in rat brain. The assay involved rapid acid proteolysis with microwave irradiation and solid-phase extraction of the free amino acids followed by LC-ESI-ITMS analysis. Detection was achieved in positive ionization with an ion trap mass spectrometer operating in multiple-reaction monitoring mode. The calibration curves of the analytes were linear (r(2) > 0.99) in the range between 0.098 and 1.560 μg/mL. Intra- and inter-day relative standard deviation percentages were <9% and <8%, respectively. The assay performance was sufficient to support a rapid analytical tool for monitoring brain protein-bound methionine oxidation levels. The content of protein-bound Met and methionine sulfoxide (MetO) in the hippocampus of adult and old rats with or without H(2)O(2) treatment was determined by employing the new method. The content of protein-bound MetO was significantly increased in old rats after exposure to H(2)O(2). This result indicates increased sensitivity to Met oxidation in the hippocampus of old rats.

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Pyrolysis mass spectra, sulfhydryl and tryptophan content of the embryonic nuclei from adult human normal and nuclear-cataractous lenses

Cited by 20 publications

References 21 publications

Degradation of crystallins from a psoriatic patient undergoing PUVA therapy

Degradation of crystallins from a psoriatic patient undergoing PUVA therapy

Guidelines for the Classification of Lenses and the Characterization of Lens Proteins

Determination of protein-bound methionine oxidationin the hippocampus of adult and old rats by LC-ESI-ITMS method after microwave-assisted proteolysis

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