Pyrococcus yayanosii sp. nov., an obligate piezophilic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent

Abstract

Pyrococcus yayanosii sp. nov., an obligate piezophilic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent Jean-Louis Birrien, 1,2,3 Xiang Zeng, 1,4 Mohamed Jebbar, 1,2,3 Marie-Anne Cambon-Bonavita, 5 Joë l Qué rellou, 5 Philippe Oger, 6,7,8 Nadè ge Bienvenu, 1,2,3 An obligate piezophilic anaerobic hyperthermophilic archaeon, designated strain CH1 T , was isolated from a hydrothermal vent site named 'Ashadze', which is located on the Mid-Atlantic Ridge at a depth of 4100 m. Enrichment and isolation of the strain were carried out at 95 6C under a hydrostatic pressure of 42 MPa. Cells of strain CH1 T were highly motile irregular cocci with a diameter of~1-1.5 mm. Growth was recorded at 80-108 6C (optimum 98 6C) and at pressures of 20-120 MPa (optimum 52 MPa). No growth was observed under atmospheric pressures at 60-110 6C. Growth was observed at pH 6.0-9.5 (optimum 7.5-8.0) and in 2.5-5.5 % (w/v) NaCl (optimum 3.5 %). Strain CH1 T was strictly anaerobic and grew on complex proteinaceous substrates, such as yeast extract, Peptone, and casein, as well as on sucrose, starch, chitin, pyruvate, acetate and glycerol without electron acceptors. The G+C content of the genomic DNA was 49.0±0.5 mol%. Analysis of 16S rRNA gene sequences revealed that strain CH1 T belongs to the genus Pyrococcus. Based on its physiological properties and similarity levels between ribosomal proteins, strain CH1 T represents a novel species, for which the name Pyrococcus yayanosii sp. nov. is proposed. The type strain is CH1 T (5JCM 16557). This strain is also available by request from the Souchothè que de Bretagne (catalogue LMBE) culture collection (collection no. 3310).Since the discovery of deep-sea hydrothermal vents and socalled black smokers, many mesophilic, thermophilic and hyperthermophilic micro-organisms of the superkingdoms Bacteria and Archaea have been described. Although vent fields have been explored at depths ranging from 800 to 3600 m, rather few attempts have been made to enrich isolates under in situ pressures. Almost all thermophilic and hyperthermophilic vent-associated prokaryotes have been isolated under atmospheric pressure and few of them have been exposed to elevated hydrostatic pressures during culture. To our knowledge, only a few piezo-thermophilic Abbreviation: CRP, concatenated ribosomal protein.

Comparative Detection and Quantification of Arcobacter butzleri in Stools from Diarrheic and Nondiarrheic People in Southwestern Alberta, Canada

“…As an internal amplification control (IAC), 2 l of DNA (1 ϫ 10 6 copies l Ϫ1 ) from a synthesized gene designed using the genome of Pyrococcus yayanosii (22) was added to the feces subsamples prior to extraction; this bacterium is an obligate piezophilic hyperthermophilic archaeon isolated from deep-sea hydrothermal sites (23). The IAC targets a 268-bp sequence in a putative carbohydrate kinase (PfkB family; GenBank accession number AEH23732.1) using the primers IAC-f (3=-GGTATGCTAGCCCCGCTTAGGGT-5=) and IAC-r (3=-TGCTCCAGAAAAGATGTCCAGCGG-5= and was synthesized by Integrated DNA Technologies.…”

Identification and Characterization of a Freshwater Pyrococcus sp. Strain PK 5017 and Identification of Pfu-Like IS Elements in Thermococcus sibiricus MM 739

“…was ≥90 o C (Fiala & Stetter, 1986;Zillig et al, 1987;Erauso et al, 1993;Gonzalez et al, 1998;Barbier et al, 1999;Birrien et al, 2011). However, the optimum temperature reported on Pyrococcus sp.…”

Pyrococcus kukulkanii sp. nov., a hyperthermophilic, piezophilic archaeon isolated from a deep-sea hydrothermal vent

“…The proximity with other known species was estimated by the comparison of concatenated ribosomal protein sequences as described previously (Matte-Tailliez et al, 2002;Brochier-Armanet et al, 2008;Birrien et al, 2011). Common ribosomal proteins (CRP) from the known members of the order Thermococcales were concatenated to form a single protein sequence of approximately 8100 residues.…”

“…The genera Pyrococcus and Palaeococcus are less abundant in molecular surveys and in culture collections. Only six species of the genus Pyrococcus have been described from shallow and deep-sea hydrothermal vents: Pyrococcus furiosus (Fiala & Stetter, 1986), Pyrococcus woesei (Zillig et al, 1987), Pyrococcus horikoshii (Gonz alez et al, 1998), 'Pyrococcus abyssi' (Erauso et al, 1993), Pyrococcus glycovorans (Barbier et al, 1999) and Pyrococcus yayanosii (Birrien et al, 2011). Two other members of the genus Pyrococcus were isolated from terrestrial environments: Pyrococcus strain HT3 from the 'El Biban' hot spring in Algeria (Kecha et al, 2007) and Pyrococcus strain Pikanate PK 5017 (Kanoksilapatham et al, 2012) from sediment collected near the Pong Duet Hot Spring in Thailand.…”