Pyrobaculum oguniense sp. nov., a novel facultatively aerobic and hyperthermophilic archaeon growing at up to 97 degrees C.

Sako, Yoshihiko; Nunoura, Takuro; Uchida, Aritsune

doi:10.1099/00207713-51-2-303

Cited by 57 publications

(42 citation statements)

References 34 publications

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“…P. oguniense (JCM10595 T ) was grown in MS medium at 90˚C and pH 7?0-7?5 as described previously (Sako et al, 2001). Aerobic batch cultures were grown in cotton-plugged 2 l Erlenmeyer flasks containing 1 l medium in an air bath rotary shaker (RGS-32.TT; Sanki Seiki, Osaka, Japan) with vigorous rotary shaking (100 r.p.m.).…”

Section: Methodsmentioning

confidence: 99%

“…Under anaerobic conditions, this organism grows with elemental sulfur, thiosulfate, glutathione (oxidized form) or L-cystine as terminal electron acceptor (Sako et al, 2001). From the aspect of respiration, the genus Pyrobaculum is uniquely variable among the hyperthermophilic archaea, which may be closest to the common ancestor of all organisms; P. aerophilum is not only a microaerophile but also a nitrate, nitrite, thiosulfate, arsenate and selenite reducer (Völkl et al, 1993;Huber et al, 2000); Pyrobaculum islandicum reduces sulfur and iron(II) compounds (Huber et al, 1987;Kashefi & Lovely, 2000;Vargas et al, 1998); Pyrobaculum organotrophum reduces sulfur compounds (Huber et al, 1987); Thermoproteus neutrophilus, which is classified in the genus Thermoproteus although the phylogenetic analysis of 16S rDNA indicated that it should be reclassified as a Pyrobaculum species (Ito et al, 1998), is a sulfur reducer (Fischer et al, 1983); Pyrobaculum arsenaticum is arsenite reducer .…”

Section: Introductionmentioning

confidence: 99%

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Regulation of the aerobic respiratory chain in the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense

et al. 2003

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulation of the aerobic respiratory chain in the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense

et al. 2003

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“…Similarly, hyperthermophilic archaea, capable of growing under an atmosphere of air such as Acidianus (Segerer et al, 1986;Zillig et al, 1986), Sulfurisphaera (Kurosawa et al, 1998), Sulfolobus (Zillig et al, 1980;Grogan et al, 1990;Jan et al, 1999;Suzuki et al, 2002) and Pyrobaculum (Sako et al, 2001;Amo et al, 2002) species isolated in terrestrial hot springs, have been never isolated from deep-sea hydrothermal environments. However, we have recently isolated strictly aerobic thermophiles, Marinithermus hydrothermalis strain T1 T and Rhodothermus sp.…”

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confidence: 99%

Aeropyrum camini sp. nov., a strictly aerobic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney

Nakagawa

Takai²,

Horikoshi³

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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A novel hyperthermophilic archaeon, designated strain SY1 T , was isolated from a deep-sea hydrothermal vent chimney sample collected from the Suiyo Seamount in the Izu-Bonin Arc, Japan, at a depth of 1385 m. The cells were irregular cocci (1?2 to 2?1 mm in diameter), occurring singly or in pairs, and stained Gram-negative. Growth was observed between 70 and 97 6C (optimum, 85 6C; 220 min doubling time), pH 6?5 and 8?8 (optimum, pH 8?0), and salinity of 2?2 and 5?3 % (optimum, 3?5 %). It was a strictly aerobic heterotroph capable of growing on complex proteinaceous substrates such as yeast extract and tryptone. The G+C content of the genomic DNA was 54?4 mol%. Phylogenetic analysis based on the 16S rDNA sequence of the isolate indicated that the isolate was closely related to Aeropyrum pernix strain K1 T . However, no significant genetic relatedness was observed between them by DNA-DNA hybridization. On the basis of the molecular and physiological traits of the new isolate, the name Aeropyrum camini sp. nov. is proposed, with the type strain SY1 T (=JCM 12091 T =ATCC BAA-758 T ).The family Desulfurococcaceae comprises eight genera, Desulfurococcus, Aeropyrum, Ignicoccus, Staphylothermus, Stetteria, Sulfophobococcus, Thermosphaera and Thermodiscus (Huber & Stetter, 2001). Members of the family Desulfurococcaceae, growing optimally at 85-95 u C, have been isolated from marine and terrestrial hydrothermal systems, and are strict anaerobes with fermentative metabolism or anaerobic respiration using sulfur or thiosulfate as electron acceptors, except for Aeropyrum pernix (Huber & Stetter, 2001). A. pernix is a strictly aerobic, heterotrophic and hyperthermophilic archaeon. The type strain of the species, strain K1 T , was isolated from a coastal solfataric thermal vent in Kodakara-Jima Island, south-western Japan (Sako et al., 1996a), and 11 additional strains were isolated from another coastal shallow hydrothermal vent and a coastal hot spring in south-western Japan (Nomura et al., 2002). In the phylum Crenarchaeota, A. pernix strain K1 T is unique in its strictly aerobic, sulfur-independent and neutrophilic growth. The complete genome sequence of this archaeon was determined for the first time in Crenarchaeota (Kawarabayasi et al., 1999), and a number of molecular biological characterizations of its hyperthermophilic enzymes have been reported (e.g. Sako et al., 1997;Yamano et al., 1999; Tachibana et al., 2000;Hansen et al., 2002;Daimon et al., 2002;Jeon & Ishikawa, 2002). Although A. pernix strain K1 T has become one of the most extensively studied archaea with respect to its molecular biology, little is known about its ecological impacts, physiological diversity and distribution in natural environments.To our knowledge, the habitats for members of the genus Aeropyrum are highly restricted to coastal geothermal fields in south-western Japan, at temperatures higher than 85 u C and neutral to weakly alkaline pH (Sako et al., 1996a; Nomura et al., 2002). Similarly, hyperthermophilic archaea, capable of growing unde...

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“…The two former strains were grown in DSMZ medium 1090, as described above. Cells of P. oguniense were grown in DSMZ medium 390 (49). In all cases, the pH value was adjusted to about 7.…”

Section: Methodsmentioning

confidence: 99%

A virus of hyperthermophilic archaea with a unique architecture among DNA viruses

Rensen

Mochizuki

Quemin

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Viruses package their genetic material in diverse ways. Most known strategies include encapsulation of nucleic acids into spherical or filamentous virions with icosahedral or helical symmetry, respectively. Filamentous viruses with dsDNA genomes are currently associated exclusively with Archaea. Here, we describe a filamentous hyperthermophilic archaeal virus, Pyrobaculum filamentous virus 1 (PFV1), with a type of virion organization not previously observed in DNA viruses. The PFV1 virion, 400 ± 20 × 32 ± 3 nm, contains an envelope and an inner core consisting of two structural units: a rod-shaped helical nucleocapsid formed of two 14-kDa major virion proteins and a nucleocapsid-encompassing protein sheath composed of a single major virion protein of 18 kDa. The virion organization of PFV1 is superficially similar to that of negative-sense RNA viruses of the family Filoviridae, including Ebola virus and Marburg virus. The linear dsDNA of PFV1 carries 17,714 bp, including 60-bp-long terminal inverted repeats, and contains 39 predicted ORFs, most of which do not show similarities to sequences in public databases. PFV1 is a lytic virus that completely disrupts the host cell membrane at the end of the infection cycle.

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Pyrobaculum oguniense sp. nov., a novel facultatively aerobic and hyperthermophilic archaeon growing at up to 97 degrees C.

Cited by 57 publications

References 34 publications

Regulation of the aerobic respiratory chain in the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense

Regulation of the aerobic respiratory chain in the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense

Aeropyrum camini sp. nov., a strictly aerobic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney

A virus of hyperthermophilic archaea with a unique architecture among DNA viruses

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