Pyrimidine salvage and catabolism in leaves of mangrove species

Sato, Yumiko; Ashihara, Hiroshi

doi:10.1016/j.plantsci.2007.10.005

Cited by 7 publications

(4 citation statements)

References 25 publications

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“…The formation of trigonelline and of nicotinic acid glucoside is, reportedly, strictly alternative in cultured cells from different plant species (Willeke et al, 1979). There are variations in nicotinic acid conjugate formation in different organs of the same plant species, however (Barz, 1985;Matsui et al, 2007;Ashihara, 2008). Potato plants produce nicotinic acid glucoside in all organs, but trigonelline is also synthesized in green organs such as leaves and stems (Katahira and Ashihara, 2009).…”

Section: Metabolism Of [Carbonyl-14 C]nicotinamidementioning

confidence: 99%

“…Although some morphological studies have been made ( Tomlinson, 1994), little is known about the metabolism of mangrove propagules (Hogarth, 1999;Krauss and Allen, 2003). We have previously investigated the purine, pyrimidine, and pyridine nucleotide metabolism in the leaves of some mangrove plants (Ashihara et al, 2003(Ashihara et al, , 2010Sato and Ashihara, 2008). However, the metabolism in individual organs of mangrove plants was determined only in Avicennia marina seedlings (Ashihara et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Nicotinamide and adenine are degradation products of NAD and adenine nucleotides, and they are reutilized in the formation of these nucleotides by so-called salvage reactions (see Stasolla et al, 2003;Zrenner and Ashihara, 2011). Secondary metabolites, such as trigonelline and allantoin, are also produced from these compounds (see Ashihara, 2008;Zrenner and Ashihara, 2011). Activities of the salvage reactions and secondary metabolite formation differ widely between plant species and organs, and are often infl uenced by environmental stress, such as salt stress (Stasolla et al, 2003;Ashihara, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Secondary metabolites, such as trigonelline and allantoin, are also produced from these compounds (see Ashihara, 2008;Zrenner and Ashihara, 2011). Activities of the salvage reactions and secondary metabolite formation differ widely between plant species and organs, and are often infl uenced by environmental stress, such as salt stress (Stasolla et al, 2003;Ashihara, 2008). In the present work, the metabolic fate of [carbonyl-14 C]nicotinamide and [8-14 C]adenine in different parts of shoot-root type emerging propagules of Bruguiera gymnorrhiza was studied so as to determine the profi le of the nucleotide metabolism in these plant materials.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic Fate of 14C-Labelled Nicotinamide and Adenine in Germinating Propagules of the Mangrove Bruguiera gymnorrhiza

Yin¹,

Watanabe²,

Ashihara³

2012

Z. Naturforsch. C

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We studied the metabolic fate of [carbonyl-14 C]nicotinamide and [8-14 C]adenine in segments taken from young and developing leaves, stem, hypocotyls, and roots of a shoot-root type emerging propagule of the mangrove plant Bruguiera gymnorrhiza. Thin-layer chromatography was used together with a bioimaging analyser system. During 4 h of incubation, incorporation of radioactivity from [carbonyl-14 C]nicotinamide into NAD and trigonelline was found in all parts of the propagules; the highest incorporation rates into NAD and trigonelline were found in newly emerged stem and young leaves, respectively. Radioactivity from [8-14 C]adenine was distributed mainly in the salvage products (adenine nucleotides and RNA), and incorporation was less in catabolites (allantoin, allantoic acid, and CO 2 ). Adenine salvage activity was higher in young leaves and stem than in hypocotyls and roots. Over a short time, the effect of 500 mM NaCl on nicotinamide and adenine metabolism indicated that NaCl inhibits both salvage and degradation activities in roots.

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Section: Metabolism Of [Carbonyl-14 C]nicotinamidementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolic Fate of 14C-Labelled Nicotinamide and Adenine in Germinating Propagules of the Mangrove Bruguiera gymnorrhiza

Yin¹,

Watanabe²,

Ashihara³

2012

Z. Naturforsch. C

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Interconversion of Pyrimidine Nucleotides

2020

Plant Nucleotide Metabolism ‐ Biosynthesis, Degradation, and Alkaloid Formation

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Brassinolide-improved development of Brassica napus microspore-derived embryos is associated with increased activities of purine and pyrimidine salvage pathways

Belmonte

Elhiti

Ashihara

et al. 2010

Planta

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Cellular brassinolide (BL) levels regulate the development of Brassica napus microspore-derived embryos (MDEs). Synthesis and degradation of nucleotides were measured on developing MDEs treated with BL or brassinazole (BrZ), a biosynthetic inhibitor of BL. Purine metabolism was investigated by following the metabolic fate of (14)C-labelled adenine and adenosine, substrates of the salvage pathway, and inosine, an intermediate of both salvage and degradation pathways. For pyrimidine, orotic acid, uridine and uracil were employed as markers for the de novo (orotic acid), salvage (uridine and uracil), and degradation (uracil) pathways. Our results indicate that utilization of adenine, adenosine, and uridine for nucleotides and nucleic acids increased significantly in BL-treated embryos at day 15 and remained high throughout the culture period. These metabolic changes were ascribed to the activities of the respective salvage enzymes: adenine phosphoribosyltransferase (EC 2.4.2.7), adenosine kinase (EC 2.7.1.20), and uridine kinase (EC 2.7.1.48), which were induced by BL applications. The BL promotion of salvage synthesis was accompanied by a reduction in the activities of the degradation pathways, suggesting the presence of competitive anabolic and catabolic mechanisms utilizing the labelled precursors. In BrZ-treated embryos, with depleted BL levels, the salvage activity of both purine and pyrimidine nucleotides was reduced and this was associated to structural abnormalities and poor embryonic performance. In these embryos, the activities of major salvage enzymes were consistently lower to those measured in their control (untreated) counterparts.

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Pyrimidine salvage and catabolism in leaves of mangrove species

Cited by 7 publications

References 25 publications

Metabolic Fate of 14C-Labelled Nicotinamide and Adenine in Germinating Propagules of the Mangrove Bruguiera gymnorrhiza

Metabolic Fate of 14C-Labelled Nicotinamide and Adenine in Germinating Propagules of the Mangrove Bruguiera gymnorrhiza

Interconversion of Pyrimidine Nucleotides

Brassinolide-improved development of Brassica napus microspore-derived embryos is associated with increased activities of purine and pyrimidine salvage pathways

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