Pyridoxine Stimulates Filaggrin Production in Human Epidermal Keratinocytes

Abstract

Pyridoxine (PN), one of the vitamers of vitamin B6, plays an important role in the maintenance of epidermal function and is used to treat acne and rough skin. Clinical studies have revealed that PN deficiency causes skin problems such as seborrheic dermatitis and stomatitis. However, the detailed effects of PN and its mechanism of action in epidermal function are poorly understood. In this study, we examined the effects of PN on epidermal function in normal human epidermal keratinocytes and found that PN specifically causes an increase in the expression of profilaggrin mRNA, among marker genes of terminal epidermal differentiation. In addition, PN treatment caused an increase in the production of filaggrin protein in a concentration-dependent manner. Treatment with P2x purinoceptor antagonists, namely, pyridoxal phosphate-6-azo (benzene-2,4-disulfonic acid) tetrasodium salt hydrate and TNP-ATP hydrate, induced an increase in the filaggrin protein levels. Moreover, we showed that elevated filaggrin production induced upon PN treatment was suppressed by ATP (known as P2x purinoceptor agonist). This study is the first to report that PN causes an increase in filaggrin transcription and production, and these results suggest that PN-induced filaggrin production may be a useful target as a daily care component in atopic dermatitis, wherein filaggrin levels are specifically reduced.

Content and stability of B complex vitamins in commercial cosmetic products

“…Similarly, topical use of dexpanthenol, the alcohol analog of pantothenic acid, is widespread for the treatment and care of damaged skin, wound healing, atopic dermatitis, dermatitis, and scar management, due to its moisturizing, anti‐inflammatory and effects on fibroblast proliferation 4 . Pyridoxine has been recently found to specifically increase filaggrin transcription and production in the skin, making it a candidate for the treatment of atopic dermatitis 5 . Biotin acts as a hair and skin‐conditioning (miscellaneous) agent and is thus commonly found in hair conditioners and masks as well as skincare products 1,6 .…”

“…4 Pyridoxine has been recently found to specifically increase filaggrin transcription and production in the skin, making it a candidate for the treatment of atopic dermatitis. 5 Biotin acts as a hair and skin-conditioning (miscellaneous) agent and is thus commonly found in hair conditioners and masks as well as skincare products. 1,6 Folic acid is an upcoming agent in the treatment of photodamaged and aged skin due to its involvement in cell replication, regulation of gene activity, and skin renewal.…”