Pyridoxamine scavenges protein carbonyls and inhibits protein aggregation in oxidative stress-induced human HepG2 hepatocytes

Dainin, Kohei; Ide, Ryoko; Maeda, Akiko; Suyama, Kyozo; Akagawa, Mitsugu

doi:10.1016/j.bbrc.2017.03.147

Cited by 12 publications

(4 citation statements)

References 31 publications

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“…Protein carbonyls increase: Carbonyl stress; production of conformationally altered polypeptide chains, which contributes to cellular dysfunction; excessive aggregation of proteins by promoting unfolding and formation of noncovalent, as well as covalent bonds between proteins; increased toxicity; may lead to apoptotic cell death [154,155].…”

Section: Oxidative Nitrosative and Sulfuric Stress In Schizophreniamentioning

confidence: 99%

Oxidative-Antioxidant Imbalance and Impaired Glucose Metabolism in Schizophrenia

et al. 2020

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Schizophrenia is a neurodevelopmental disorder featuring chronic, complex neuropsychiatric features. The etiology and pathogenesis of schizophrenia are not fully understood. Oxidative-antioxidant imbalance is a potential determinant of schizophrenia. Oxidative, nitrosative, or sulfuric damage to enzymes of glycolysis and tricarboxylic acid cycle, as well as calcium transport and ATP biosynthesis might cause impaired bioenergetics function in the brain. This could explain the initial symptoms, such as the first psychotic episode and mild cognitive impairment. Another concept of the etiopathogenesis of schizophrenia is associated with impaired glucose metabolism and insulin resistance with the activation of the mTOR mitochondrial pathway, which may contribute to impaired neuronal development. Consequently, cognitive processes requiring ATP are compromised and dysfunctions in synaptic transmission lead to neuronal death, preceding changes in key brain areas. This review summarizes the role and mutual interactions of oxidative damage and impaired glucose metabolism as key factors affecting metabolic complications in schizophrenia. These observations may be a premise for novel potential therapeutic targets that will delay not only the onset of first symptoms but also the progression of schizophrenia and its complications.

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Section: Oxidative Nitrosative and Sulfuric Stress In Schizophreniamentioning

confidence: 99%

Oxidative-Antioxidant Imbalance and Impaired Glucose Metabolism in Schizophrenia

et al. 2020

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“…MDA, a common lipid peroxidation agent and an indicator for lipid peroxidation of the cell membrane, had certain cytotoxicity. PCO, a sign of oxidative damage of proteins, could cause conformational changes in the polypeptide chain, resulting in a loss of protein function . MPO produced by neutrophils could promote oxidative stress damage by activating chlorine molecules .…”

Section: Resultsmentioning

confidence: 99%

Orientin Attenuatedd-GalN/LPS-Induced Liver Injury through the Inhibition of Oxidative Stress via Nrf2/Keap1 Pathway

Liao

Ling

et al. 2022

J. Agric. Food Chem.

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Oxidative stress is involved in the pathogenesis of liver diseases, including liver injury, a serious health problem worldwide. Natural polyphenols have attracted increasing attention as potential agents for the prevention and treatment of liver diseases. Orientin, a flavonoid component with antioxidant capacity, has been regarded as a promising nutraceutical for patients with liver damage. This study aimed to investigate the amelioration effect of orientin on D-galactosamine and lipopolysaccharides (D-GalN/LPS) induced liver injury in mice, with a focus on its underlying mechanisms by using the H 2 O 2 -induced oxidative damage model of HepG2 cells. Results indicated that orientin alleviated D-GalN/LPS-induced liver damage by improving the hepatic histological changes and reducing the levels of hepatic and serum alanine aminotransferase and aspartic acid aminotransferase. Additionally, supplementation of orientin improved the antioxidant ability in mice by decreasing the levels of hepatic malondialdehyde, protein carbonyl, myeloperoxidase, nitric oxide, glutathione, glutathione peroxidase, gluathione reductase, and superoxide dismutase. Orientin treatment significantly elevated both the protein and mRNA expressions of nuclear factor erythroid 2-related factor 2, Kelch-like ECH-associated protein-1, heme oxygenase-1, and nicotinamide quinone oxidoreductase 1 in liver and HepG2 cells. The management of orientin also elevated the protein expression of glutathione S-transferase and Maf in HepG2 cells. Taken together, it suggested that orientin played an amelioration effect on liver injury by suppressing oxidative stress, which might be strongly related to the activation of Nrf2/ARE through PI3K/Akt and P38/MAPK signal pathways.

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“…Oxidative stress leads to the non-enzymatic modification of specific amino acid residues, where aldehyde or ketone functional groups are introduced, this is known as carbonylation [21]. Protein carbonylation can have deleterious effects on cell function, since it can lead to protein dysfunction and to the production of potentially harmful protein aggregates [22]. In addition, Protein carbonyl groups can be used as biomarkers for oxidative damage to proteins [23].…”

Section: Introductionmentioning

confidence: 99%

Expression Pattern of Mitogen-inducible Gene 6 in Relation to the Extracellular Signal-regulated Kinase Pathway and Redox Status in Patients with Endometriosis

Ayad

Gaballah

Salem

et al. 2020

AJBGMB

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Background: Endometriosis is a gynecological disorder characterized by the presence of ectopic endometrium outside the uterus. It is a multi-factorial disease, where different processes facilitate the implantation and survival of ectopic endometrial tissue. The Aim: This study aims to evaluate the levels of the expression patterns of mitogen-inducible gene 6 (Mig-6) in eutopic and ectopic endometrial tissues of endometriosis patients and their relevance to the levels of phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2) and redox status. Methods: Relative gene expression levels of Mig-6 and the levels of pERK1/2 were assessed in endometrial tissues of control group and in both eutopic and ectopic endometrial tissues of endometriosis patients .Serum levels of total protein thiols and total protein carbonyls were also assessed in endometriosis and control groups. Results: Relative gene expression levels of Mig- 6 showed significant lower values in endometriosis group as compared to control group, and they were associated with significant increase in pERK 1/2 levels and altered redox status. Conclusion: These data, signified a significant role of downregulated Mig-6 expression, and activated ERK 1/2 signaling in the pathogenesis of endometriosis. Moreover, it suggested that oxidative stress indicated by high protein carbonyls level contributes to the pathogenesis of endometriosis and that protein thiols have a defensive role against oxidative stress.

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Pyridoxamine scavenges protein carbonyls and inhibits protein aggregation in oxidative stress-induced human HepG2 hepatocytes

Cited by 12 publications

References 31 publications

Oxidative-Antioxidant Imbalance and Impaired Glucose Metabolism in Schizophrenia

Oxidative-Antioxidant Imbalance and Impaired Glucose Metabolism in Schizophrenia

Orientin Attenuatedd-GalN/LPS-Induced Liver Injury through the Inhibition of Oxidative Stress via Nrf2/Keap1 Pathway

Expression Pattern of Mitogen-inducible Gene 6 in Relation to the Extracellular Signal-regulated Kinase Pathway and Redox Status in Patients with Endometriosis

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