Pyridoxal Phosphate (B6-al-PO4) Levels in Organs, Leukocytes and Blood of Rats with Developing Vit. B6 Deficiency.

Abstract

9015that increased weight gain in hypophysecte mized animals was achieved despite a significantly smaller quantity of f d ingested. When weight gains are corrected for amount of food ingested, increases in weight of the handled over unhandled group are greatly magnified. In the normal rat, handling has no influence on amount of food ingested( 6). The findings indicate that handling increases efficiency of food utilization (increased intestinal absorption and/or diminished metabdism) and that this effect is not dependent upon presence of pituitary or adrenal hormoines.It is apparent that increased skeletal growth previously reported in the handled normal young rat (6), is abolished by hypophysectomy. This suggests that the effect of handling on skeletal growth requires pituitary hormones, presumably growth hormone and/or thyrotropic hormone.It is pertinent to consider the contribution of handling to weight gains previously reported in hypophysectomized rats treated with insulin ( 13 ) or force-fed ( 14). Since these studies involved extra handling of experimental animals as compared with control animals, it is conceivable that weight gains observed were due in part to extra handling.Summary. Handling in young hypophysectomized rats resulted in significantly increased weight gain despite ingestion of significantly less food. No effect on skeletal growth or percentage of carcass protein and fat was observed. Increased weight gain in the handled hypophysectomized rat is due to greater efficiency of intestinal absorption of food and/or diminished metabolism rather than a direct stimulation of growth or increased food ingestion.We wish to acknowledge the {technical assistance of Y.

Effect of Postweanling Pyridoxine Deficiency on Growth and Concentration of the Coenzyme Pyridoxal-5′-phosphate in Heart, Kidneys, Lungs, and Adrenals in Rats

“…and adrenals being more severely affected than the heart. Data on the P L P content of heart and kidneys have been reported previously by Wachstein and Moore (29), but the values for the control tissues are much lower than ours. This may be because of methodologic differences, especially with respect to the extraction and assay of PLP.…”

Water-Soluble Vitamins, Part II (Vitamin B12, Folic Acid, Ascorbic Acid, Biotin, Vitamin B6, Miscellaneous)

“…of human blood. Wachstein et al (149,150) have used Boxer's method to measure pyridoxal phosphate in the leucocytes of pregnant women (149), and in the blood and tissues of rats with developing vitamin Be deficiency (150). An alternative method based on the coenzyme properties of pyridoxal phosphate was developed by Wada, Morisue, Sakamoto & Ichihara (151).…”