1977
DOI: 10.1016/0003-9861(77)90346-0
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Pyridine nucleotide distributions and enzyme mass action ratios in hepatocytes from fed and starved rats

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Cited by 201 publications
(109 citation statements)
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“…Regarding the absolute intramitochondrial concentrations of ketone bodies, our results agree with those of Tischler et al [30], amounting to 3-5 m M in the absence and presence of oleate, respectively. Moreover, these authors also noticed no concentration gradient between the cytosol and the extracellular medium, yet the value for the gradient between the cytosolic and mitochondrial compartments was even higher than that described herein.…”
Section: Subcehlur Distribution Of Ketone Bodiessupporting
confidence: 92%
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“…Regarding the absolute intramitochondrial concentrations of ketone bodies, our results agree with those of Tischler et al [30], amounting to 3-5 m M in the absence and presence of oleate, respectively. Moreover, these authors also noticed no concentration gradient between the cytosol and the extracellular medium, yet the value for the gradient between the cytosolic and mitochondrial compartments was even higher than that described herein.…”
Section: Subcehlur Distribution Of Ketone Bodiessupporting
confidence: 92%
“…In support of this is the fact that the cytosolic concentration of acetoacetate of 0.6-1 mM (see Fig.4B) is around the K , reported for acetoacetate transport of 0.6 mM [34]. Moreover, the intramitochondrial concentration of acetoacetate is higher by a factor of about 2 than that calculated [35] for a mere distribution according to the pH difference of 0.4 [17, 30,36] between the mitochondrial and cytosolic spaces. This is the more noteworthy, as 3-hydroxybutyrate under all conditions tested has been found to be distributed (see Fig.…”
Section: Subcehlur Distribution Of Ketone Bodiessupporting
confidence: 56%
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“…This conclusion is in agreement with measurements made of intracellular lactate and pyruvate concentrations in hepatocytes under conditions of rapid gluconeogenesis (Groen et al, 1983). Our data also confirm the widely held view that lactate, pyruvate, and the ketone bodies equilibrate across the liver cell plasma membrane, allowing extracellular lactate/pyruvate and ␤-hydroxybutyrate/acetoacetate ratios to be used to estimate cytosolic and mitochondrial NADH/NAD ϩ ratios, respectively (see Tischler et al (1977), Groen et al (1983), and Poole and Halestrap (1993) …”
supporting
confidence: 90%
“…Glucose was determined using the glucose oxidase method [16] and both lactate [17] and pyruvate [18] were assayed in extracts of incubated whole cells. Previous studies have shown that similar ratios of lactate to pyruvate are found in the cytosol, whole cells, or the suspending medium [19,20]. Urea was measured in deproteinized extracts using urease and glutamate dehydrogenase…”
Section: Metabolite Determinationsmentioning
confidence: 99%