Pyridine boronic acid-polyoxometalate based porous hybrid for efficient depletion of high abundant glycoproteins in plasma

Hu, Xue; Qing, Chen; Zhang, Dandan; Chen, Xuwei; Wang, Jianhua

doi:10.1039/c8tb02265h

Cited by 12 publications

(3 citation statements)

References 44 publications

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“…As shown in Figure A, the binding capacity of PAN/DA@PDA@APBA/Ni toward OVA increased quickly along with the increase of OVA concentration, and the maximum adsorption capacity could reach 604.6 mg/g. Compared with other reported glycoprotein adsorbents, − PAN/DA@PDA@APBA/Ni has a notably superior capacity for glycoprotein adsorption (Table ).…”

Section: Resultsmentioning

confidence: 82%

Electrospun Fiber Membrane with the Dual Affinity of Chelation and Covalent Interactions for the Efficient Enrichment of Glycoproteins

Liu,

Lu,

Liu

et al. 2024

ACS Appl. Bio Mater.

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As important biomarkers of many diseases, glycoproteins are of great significance to biomedical science. It is essential to develop efficient glycoprotein enrichment platforms and investigate their adsorption mechanism. In this work, a conspicuous enrichment strategy for glycoproteins was developed by using an electrospun fiber membrane wrapped with polydopamine (PDA) and modified with 3-aminophenylboronic acid and nickel ions, named PAN/DA@PDA@APBA/Ni. The enrichment characteristics of PAN/DA@PDA@APBA/Ni toward glycoproteins were explored through adsorption behavior. Thanks to the existence of two sites of interaction (metal ion chelation and boronate affinity), PAN/DA@PDA@APBA/Ni exhibited significant enrichment capacity for glycoproteins, ovalbumin (604.6 mg/g), and human immunoglobulin G (331.0 mg/g). The adsorption kinetic results of glycoprotein ovalbumin on PAN/DA@PDA@APBA/Ni conform to the pseudo-first-order kinetic model in the first adsorption stage, while the second half adsorption stage is more in line with the pseudo-second-order kinetic model. Moreover, the physical characteristics of PAN/DA@PDA@APBA/Ni and subsequent adsorption experiments on electrospun fiber modified with only phenylboronic acid or nickel ions both confirmed two sites of interaction (metal ion chelation and boronate affinity, respectively). Furthermore, a stepwise elution method with dual-affinity interaction was designed and successfully applied to enrich glycoproteins in real biological samples. This work provides an idea for sample pretreatment, especially for the design of dual-affinity materials in glycoproteins enrichment.

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Section: Resultsmentioning

confidence: 82%

Electrospun Fiber Membrane with the Dual Affinity of Chelation and Covalent Interactions for the Efficient Enrichment of Glycoproteins

Liu,

Lu,

Liu

et al. 2024

ACS Appl. Bio Mater.

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“…Not only are these methods used for enrichment of glycoproteins, but similar techniques have also been used for depletion of these compounds when protein analysis is desired as illustrated by the use of pyridine boronic acid‐polyoxometalate based porous hybrid for efficient depletion of high abundant glycoproteins in plasma (Hu et al, 2018).…”

Section: Studies On Specific Carbohydrate Typesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Harvey

2021

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2018. Also included are papers that describe methods appropriate to glycan and glycoprotein analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, new methods, matrices, derivatization, MALDI imaging, fragmentation and the use of arrays. The second part of the review is devoted to applications to various structural types such as oligo‐ and poly‐saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Most of the applications are presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. The reported work shows increasing use of combined new techniques such as ion mobility and highlights the impact that MALDI imaging is having across a range of diciplines. MALDI is still an ideal technique for carbohydrate analysis and advancements in the technique and the range of applications continue steady progress.

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“…Selective hydrolytic cleavage of peptide bonds is a strategy for highly specific molecular recognition and genetic targeting, which is particularly important in proteomics, , protein engineering, mapping of enzyme active sites, and digestion of pathogenic proteins . Despite the wide application of these fields, natural proteases suffer from weak selectivity, suffer from pH/temperature limitations, and are not economically cost-effective. , The need to streamline the analysis of protein structure and function determination has led to the development of selective artificial proteases as a new chemical approach for controlled and selective protein cleavage .…”

Section: Introductionmentioning

confidence: 99%

Selective Hydrolysis of Ovalbumin by Zr-Based Lacunary Polyoxotungstate in Surfactant Solutions

Babaei Zarch,

Bazargan,

Mirzaei

2024

Inorg. Chem.

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This study aims to design an artificial metalloprotease based on a Zr-containing polyoxometalate Na 8 ) 2 ] [Zr(W 5 ) 2 ] for the hydrolysis of ovalbumin (OVA) in the presence of different surfactants, which can be used in many areas of the biological and medical sciences, particularly for targeted proteolytic drug design. For this reason, parameters, including the free energy of binding, the chemical nature of amino acid residues, secondary structures, and electrostatic potentials, of Zr(W 5 ) 2 -OVA and Zr(W 5 ) 2 -OVA-surfactant were analyzed by molecular docking simulations. The investigations showed that the presence of surfactants decreases the binding affinity of Zr(W 5 ) 2 for OVA amino acids, and hydrogen bonds and van der Waals interactions are formed between Zr(W 5 ) 2 and OVA amino acids. Additionally, GROMACS further illustrated the significance of SDS and CTAB surfactants in influencing the conformational changes of the OVA that lead to selective protein hydrolysis. In agreement with molecular dynamics simulation results, the experimental analysis showed more protein hydrolysis for the Zr(W 5 ) 2 -OVA-surfactant systems. For instance, circular dichroism spectroscopy indicated that Zr(W 5 ) 2 -OVA-CTAB and Zr(W 5 ) 2 -OVA-TX-100 were more hydrolytically efficient due to the increased level of βstructures rather than α-chains, which showed that surfactants can facilitate the accessibility of Zr(W 5 ) 2 to the cleavage sites by inducing partial unfolding of the OVA structure.

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Pyridine boronic acid-polyoxometalate based porous hybrid for efficient depletion of high abundant glycoproteins in plasma

Abstract: A porous hybrid, namely PW12@TiO2–Si(Et)Si/Pba, is fabricated by the modification of PW12@TiO2–Si(Et)Si with pyridine boronic acid and used for glycoprotein depletion.

Cited by 12 publications

References 44 publications

Electrospun Fiber Membrane with the Dual Affinity of Chelation and Covalent Interactions for the Efficient Enrichment of Glycoproteins

Electrospun Fiber Membrane with the Dual Affinity of Chelation and Covalent Interactions for the Efficient Enrichment of Glycoproteins

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018

Selective Hydrolysis of Ovalbumin by Zr-Based Lacunary Polyoxotungstate in Surfactant Solutions

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