Pyrethroids Use: Threats on Metabolic-Mediated Resistance Mechanisms in the Primary Dengue Vector Aedes aegypti (Diptera: Culicidae)

Amelia-Yap, Zheng Hua; Sofian‐Azirun, Mohd; Suana, I Wayan; Elia-Amira, N M R; Haziqah-Rashid, A; Tan, Tiong Kai; Lim, Yvonne Ai Lian; Low, Van Lun

doi:10.1093/jme/tjz007

Cited by 11 publications

(12 citation statements)

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“…Increased levels of MFOs in Ae. aegypti related to pyrethroids resistance were reported elsewhere (Maestre-Serrano et al, 2014;Rasli et al, 2018;Triana et al, 2019;Amelia-Yap et al, 2019). Nonetheless, the increased MFO activity in most mosquito populations might reduce the efficacy of insecticides.…”

Section: Discussionmentioning

confidence: 89%

“…quinquefasciatus Corbel et al, 2007;Sarkar et al, 2009;Low et al, 2013aLow et al, , 2013b. To date, associations between insecticide resistance and GST enzyme activity have not been fully identified in a variety of mosquito species worldwide (Amelia- Yap et al, 2019). The detectable GST activity might be owing to the use of pyrethroids in mosquito control activities as both DDT and pyrethroids intended to target the voltage-gated sodium channel of arthropods (Amelia- Yap et al, 2018bYap et al, , 2019Hemingway & Ranson, 2000;Koou et al, 2014b).…”

Section: Discussionmentioning

confidence: 99%

“…To date, associations between insecticide resistance and GST enzyme activity have not been fully identified in a variety of mosquito species worldwide (Amelia- Yap et al, 2019). The detectable GST activity might be owing to the use of pyrethroids in mosquito control activities as both DDT and pyrethroids intended to target the voltage-gated sodium channel of arthropods (Amelia- Yap et al, 2018bYap et al, , 2019Hemingway & Ranson, 2000;Koou et al, 2014b). Thus, it assumed that pyrethroid-resistant identified in this study was related to the metabolic detoxification or/and target-site insensitivity.…”

Section: Discussionmentioning

confidence: 99%

“…The Ae. aegypti Bora-Bora strain obtained from the Universiti Sains Malaysia, same as those in Amelia- Yap et al (2018aYap et al ( , 2019, was used as the susceptible reference population.…”

Section: Colonization Of Aedes Aegyptimentioning

confidence: 99%

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Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

et al. 2021

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This study aims to examine the efficacy of mosquito mat vaporizers on Aedes aegypti and their associated metabolic detoxication mechanisms. For this purpose, Aedes aegypti (Linnaeus) was collected from nine districts in Selangor, Malaysia and tested with mosquito vaporizing mat bioassays. The same populations were also subjected to biochemical assays to investigate activities of detoxifying enzymes, namely non-specific esterase (EST), glutathione-S-transferase (GST) and mixed function oxidase (MFO). The efficacy of Ae. aegypti on the active ingredients tested in decreasing order were d-allethrin > dimefluthrin > prallethrin with PBO > prallethrin. The results further indicated significant enhancement mean levels of EST, GST and MFO in pyrethroid-resistant populations. The mortality rate of Ae. aegypti in response to pyrethroid active ingredients was associated with MFO activity, suggesting it is an important detoxification enzyme for the populations tested. In view of the presence of resistance against household insecticide products, pyrethroid efficacy on Ae. aegypti populations needs to be monitored closely to ensure the implementation of an effective vector control program in Malaysia.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Colonization Of Aedes Aegyptimentioning

confidence: 99%

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Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

et al. 2021

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“…However, ESTs and GSTs have also been implicated in pyrethroid resistance in Ae. aegypti populations globally [9,[12][13][14]25,27,32,33]. For ESTs, it is a series of reactions causing esterase hydrolysis of pyrethroids that results in insecticide detoxification [9].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Resistance in Permethrin-Resistant Florida Aedes aegypti (Diptera: Culicidae)

Schluep¹,

Buckner²

2021

Insects

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Aedes aegypti is the principal mosquito vector for many arthropod-borne viruses (arboviruses) including dengue, chikungunya, and Zika. In the United States, excessive permethrin use has led to a high frequency of resistance in mosquitoes. Insecticide resistance is a significant obstacle in the struggle against vector-borne diseases. To help overcome metabolic resistance, synergists that inhibit specific metabolic enzymes can be added to formulated pyrethroid products. Using modified CDC bottle bioassays, we assessed the effect of three inhibitors (piperonyl butoxide (PBO), which inhibits oxidase activity; S.S.S-tributyl phosphorotrithioate (DEF), which inhibits esterase activity; and diethyl maleate (DM), which inhibits glutathione transferase activity) + permethrin. We performed these against 20 Florida Ae. aegypti populations, all of which were resistant to permethrin. Our data indicated that 11 out of 20 populations (55%) exhibited metabolic resistance. Results revealed 73% of these populations had significant increases in mortality attributed to DEF + permethrin, 64% to PBO + permethrin, and 55% to DM + permethrin compared to permethrin alone. Currently, PBO is the only metabolic enzyme inhibitor added to formulated pyrethroid products used for adult mosquito control. Our results suggest that the DEF and DM inhibitors could also be useful additives in permethrin products, especially against metabolically resistant Ae. aegypti mosquitoes. Moreover, metabolic assays should be conducted to better inform mosquito control programs for designing and implementing integrated vector management strategies.

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Insecticide resistance compromises the control of Aedes aegypti in Bangladesh

Al-Amin

Gyawali

Graham

et al. 2023

Pest Management Science

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BACKGROUND With no effective drugs or widely available vaccines, dengue control in Bangladesh is dependent on targeting the primary vector Aedes aegypti with insecticides and larval source management. Despite these interventions, the dengue burden is increasing in Bangladesh, and the country experienced its worst outbreak in 2019 with 101 354 hospitalized cases. This may be partially facilitated by the presence of intense insecticide resistance in vector populations. Here, we describe the intensity and mechanisms of resistance to insecticides commonly deployed against Ae. aegypti in Dhaka, Bangladesh. RESULTS Dhaka Ae. aegypti colonies exhibited high‐intensity resistance to pyrethroids. Using CDC bottle assays, we recorded 2–24% mortality (recorded at 24 h) to permethrin and 48–94% mortality to deltamethrin, at 10× the diagnostic dose. Bioassays conducted using insecticide–synergist combinations suggested that metabolic mechanisms were contributing to pyrethroid resistance, specifically multi‐function oxidases, esterases, and glutathione S‐transferases. In addition, kdr alleles were detected, with a high frequency (78–98%) of homozygotes for the V1016G mutation. A large proportion (≤ 74%) of free‐flying and resting mosquitoes from Dhaka colonies survived exposure to standard applications of pyrethroid aerosols in an experimental free‐flight room. Although that exposure affected the immediate host‐seeking behavior of Ae. aegypti, the effect was transient in surviving mosquitoes. CONCLUSION The intense resistance characterized in this study is likely compromising the operational effectiveness of pyrethroids against Ae. aegypti in Dhaka. Switching to alternative chemical classes may offer a medium‐term solution, but ultimately a more sustainable and effective approach to controlling dengue vectors is required. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Pyrethroids Use: Threats on Metabolic-Mediated Resistance Mechanisms in the Primary Dengue Vector Aedes aegypti (Diptera: Culicidae)

Cited by 11 publications

References 13 publications

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

Metabolic Resistance in Permethrin-Resistant Florida Aedes aegypti (Diptera: Culicidae)

Insecticide resistance compromises the control of Aedes aegypti in Bangladesh

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