Pyrene-Labelled Lipids as Fluorescent Probes in Studies on Biomembranes and Membrane Models

Kinnunen, Paavo K.J.; Kõiv, Anu; Mustonen, Pekka

doi:10.1007/978-3-642-77372-3_12

Cited by 45 publications

(46 citation statements)

References 69 publications

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“…Accordingly, this parameter can be used to observe phase separation and changes in the dynamics of bilayers upon heating (Kinnunen et al, 1993;Duportail and Lianos, 1996). At 25 • C, POPC bilayers are in the fluid state, and no segregation, whatsoever, for PPDPC is observed.…”

Section: Effects Of Lbpa 18:1 On the Dynamics Of Popc Bilayersmentioning

confidence: 99%

“…Essentially, the excimer to monomer fluorescence intensity ratio (I e /I m ) is proportional to the rate of collisions between the pyrenes. For a single pyrene moiety containing lipid analog such as PPDPC, the value for I e /I m reflects the lateral mobility as well as the local concentration of the fluorophore in the membrane (Kinnunen et al, 1993). At sufficiently low probe concentrations, excimer fluorescence of dipyrene phospholipids such as bisPDPC, containing a pyrene moiety at both ends of its acyl chains, is intramolecular and concentration-independent (Sunamoto et al, 1980).…”

Section: Measurement Of I E /I M For Ppdpc and Bispdpcmentioning

confidence: 99%

See 1 more Smart Citation

Intermolecular interactions of lysobisphosphatidic acid with phosphatidylcholine in mixed bilayers

Holopainen

Söderlund

Alakoskela

et al. 2005

Chemistry and Physics of Lipids

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Section: Effects Of Lbpa 18:1 On the Dynamics Of Popc Bilayersmentioning

confidence: 99%

Section: Measurement Of I E /I M For Ppdpc and Bispdpcmentioning

confidence: 99%

Intermolecular interactions of lysobisphosphatidic acid with phosphatidylcholine in mixed bilayers

Holopainen

Söderlund

Alakoskela

et al. 2005

Chemistry and Physics of Lipids

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“…Because of the low concentrations of both lipids and cyt c generally utilized, minimal interference by the inner filter effect was expected. The merits as well as limitations of the use of pyrene-labeled lipids in energy transfer measurements have been discussed elsewhere (22,(33)(34)(35).…”

Section: Materials-1-palmitoyl-2-[10-(pyren-1-yl)decanoyl]-sn-glycero-3-mentioning

confidence: 99%

ATP Induces a Conformational Change in Lipid-bound Cytochrome c

Tuominen

Zhu

Wallace

et al. 2001

Journal of Biological Chemistry

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“…Of course, the unstructured emission at higher temperatures is due to excimer formation between the pyrene groups. 37 If the pyrenyl-PC is diluted into membranes, the amount of excimer formation can be used to estimate the rate of lateral diffusion of lipids in the membranes.…”

Section: 2e Membrane Probesmentioning

confidence: 99%

Fluorophores

Lakowicz¹

1999

Principles of Fluorescence Spectroscopy

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FluorophoresFluorescence probes represent the most important area of fluorescence spectroscopy. One can spend a great deal of time describing the instrumentation for fluorescence spectroscopy, including light sources, monochromators, lasers, and detectors. However, in the final analysis, the wavelength and time resolution required of the instruments are determined by the spectral properties of the fluorophores. Furthermore, the information available from the experiments is determined by the properties of the probes. Only probes with nonzero anisotropies can be used to measure rotational diffusion, and the lifetime of the fluorophore must be comparable to the correlation time of interest. Only probes which are sensitive to pH can be used to measure pH. And only probes with reasonably long excitation and emission wavelengths can be used with tissues which display autofluorescence at short excitation wavelengths.Thousands of fluorescent probes are known, and it is not practical to describe them all. This chapter contains an overview of the various types of fluorophores, their spectral properties, and their applications. Fluorophores can be broadly divided into two main classes, intrinsic and extrinsic. Intrinsic fluorophores are those which occur naturally. These include the aromatic amino acids, NADH, flavins, and derivatives of pyridoxal and chlorophyll. Extrinsic fluorophores are added to the sample to provide fluorescence when none exists or to change the spectral properties of the sample. Extrinsic fluorophores include dansyl chloride, fluorescein, rhodamine, and numerous other substances. 3.1.A. Fluorescent Enzyme CofactorsEnzyme cofactors frequently are fluorescent (Figure 3.1). NADH is highly fluorescent, with absorption and emission maxima at 340 and 460 nm, respectively (Figure 3.3). The oxidized form of NADH, NAD+, is nonfluorescent. The lifetime of NADH in aqueous buffer is near 0.4 ns. The 63 J. R. Lakowicz, Principles of Fluorescence Spectroscopy

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Pyrene-Labelled Lipids as Fluorescent Probes in Studies on Biomembranes and Membrane Models

Cited by 45 publications

References 69 publications

Intermolecular interactions of lysobisphosphatidic acid with phosphatidylcholine in mixed bilayers

Intermolecular interactions of lysobisphosphatidic acid with phosphatidylcholine in mixed bilayers

ATP Induces a Conformational Change in Lipid-bound Cytochrome c

Fluorophores

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