Pyrene Excimer Nucleic Acid Probes for Biomolecule Signaling

Wu, Cuichen; Wang, Chunming; Yan, Ling; Yang, Chaoyong

doi:10.1166/jbn.2009.1074

Cited by 43 publications

(20 citation statements)

References 36 publications

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“…With the addition of IgE, the peak at 485 nm gradually increased, corresponding to the binding-induced generation of the pyrene excimer. For the emission of the monomer, it did not show any decrease, which is different from that usually reported, 31,32,35 and it increased with the addition of IgE. This kind of phenomenon was also observed in some assays using the aptamer excimer probe, 36,41 but the exact reason remains unknown.…”

Section: Detection Of Igecontrasting

confidence: 75%

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Rapid fluorescence detection of immunoglobulin E using an aptamer switch based on a binding-induced pyrene excimer

Bai

Zhao

2017

Anal. Methods

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Immunoglobulin E (IgE) is an important immunoglobulin related to allergic reactions and some diseases such as atopic dermatitis, allergic asthma, etc. Sensitive and rapid detection of IgE is desired for disease diagnosis and therapeutics. Taking advantage of a binding-induced pyrene excimer, we developed a sensitive fluorescent aptamer switch for IgE sensing by using a pyrene-labeled aptamer. The aptamer having four base pairs at the stem was labeled with a single pyrene molecule at the 3 0 end and 5' end and used as an affinity probe. The binding of IgE brought the pyrene moiety at each terminal of the aptamer into proximity, generating an excimer and showing a distinct fluorescence peak. The measurement of the fluorescence peak of the excimer allowed the sensitive detection of IgE. We optimized the experimental conditions such as temperature, incubation time, and metal cations. The detection limit for IgE was about 1.6 nM. This assay displayed good specificity and allowed for the detection of IgE spiked in a diluted human serum sample. This fluorescent aptamer sensor has potential for its application in rapid sensing of IgE.

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Section: Detection Of Igecontrasting

confidence: 75%

“…[29][30][31][32][33][34][35] The pyrene molecules in proximity generate uorescence peaks different from those of separated pyrenes. The combination of the aptamer and pyrene excimer provides an aptamer excimer probe for rapid detection of targets based on target-binding induced structure switching of the aptamer.…”

Section: Introductionmentioning

confidence: 99%

Rapid fluorescence detection of immunoglobulin E using an aptamer switch based on a binding-induced pyrene excimer

Bai

Zhao

2017

Anal. Methods

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“…4,5,15 In this regard, intermolecular interactions in the excited states attract attention increasingly. 2,3,8,10,[16][17][18][19][20][21][22][23][24][25][26] As for recent theoretical studies of intermolecular interactions in the excited states, various acene molecules including benzene were employed. 9,18,[20][21][22][24][25][26][27] Most of those studies, however, rely on the frozen molecular structures derived from the ground-state calculations and thus the geometric relaxation effects have been neglected.…”

Section: 5mentioning

confidence: 99%

“…They are known to play instrumental roles in various biological phenomena [1][2][3] and supramolecular chemistry.…”

Section: Introductionmentioning

confidence: 99%

A Theoretical Study of the Formation of Benzene Excimer: Effects of Geometry Relaxation and Spin-state Dependence

Kim¹

2014

Bulletin of the Korean Chemical Society

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Geometry relaxation effects on the formation of benzene excimer were investigated by means of ab initio calculation at SOS-CIS(D 0 )/aug-cc-pVDZ level. In the case of T-shaped dimer configuration, intermolecular interactions in the excited states are found to be nearly the same as those in the ground state and structural deformations are limited within a single molecule; the geometry relaxation effects are then negligible and singlet-triplet energy gap remains constant. As for face-to-face eclipsed dimer, on the other hand, both molecules undergo structural change. As a result, intermolecular interactions in the excited states are significantly different than those in the ground state. Although the intermolecular distances obtained from potential energy curve calculation with frozen molecular structures are in qualitative agreement, the excitedstate binding energies are notably overestimated with respect to those at optimized structures. In particular, the effects are calculated to be larger in T 1 state and hence singlet-triplet energy gap, which reduces markedly in this configuration, is underestimated without relaxation.

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“…The finding that fluorescence of excimer and monomer pyrene is sensitive to local environment changes [57,58] has been employed for aptamer-based biosensing. In an early report, it was found that incorporation of a bis-pyrenyl fluorophore at a site adjacent to the ATP-binding residues within an aptamer exhibited a significant increase of the excimer fluorescence signal intensity upon ATP binding, which could be used to detect mM concentrations of ATP [59].…”

Section: Biosensors Based On Fluorescently-labeled Aptamersmentioning

confidence: 99%

Aptamer-Based Fluorescent Biosensors

Wang

Zhang²,

Cai³

et al. 2011

CMC

178

107

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Selected from random pools of DNA or RNA molecules through systematic evolution of ligands by exponential enrichment (SELEX), aptamers can bind to target molecules with high affinity and specificity, which makes them ideal recognition elements in the development of biosensors. To date, aptamer-based biosensors have used a wide variety of detection techniques, which are briefly summarized in this article. The focus of this review is on the development of aptamer-based fluorescent biosensors, with emphasis on their design as well as properties such as sensitivity and specificity. These biosensors can be broadly divided into two categories: those using fluorescently-labeled aptamers and others that employ label-free aptamers. Within each category, they can be further divided into “signal-on” and “signal-off” sensors. A number of these aptamer-based fluorescent biosensors have shown promising results in biological samples such as urine and serum, suggesting their potential applications in biomedical research and disease diagnostics.

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Pyrene Excimer Nucleic Acid Probes for Biomolecule Signaling

Cited by 43 publications

References 36 publications

Rapid fluorescence detection of immunoglobulin E using an aptamer switch based on a binding-induced pyrene excimer

Rapid fluorescence detection of immunoglobulin E using an aptamer switch based on a binding-induced pyrene excimer

A Theoretical Study of the Formation of Benzene Excimer: Effects of Geometry Relaxation and Spin-state Dependence

Aptamer-Based Fluorescent Biosensors

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