Pyramiding of drought yield QTLs into a high quality Malaysian rice cultivar MRQ74 improves yield under reproductive stage drought

Abstract

BackgroundWith the objective of improving the grain yield (GY) of the Malaysian high quality rice cultivar MRQ74 under reproductive stage drought stress (RS), three drought yield QTLs, viz. qDTY2.2, qDTY3.1, and qDTY12.1 were pyramided by marker assisted breeding (MAB). Foreground selection using QTL specific markers, recombinant selection using flanking markers, and background selections were performed in every generation. BC1F3 derived pyramided lines (PLs) with different combinations of qDTY2.2, qDTY3.1, and qDTY12.1 were evaluated under both RS and non-stress (NS) during the dry season (DS) of 2013 and 2014 at IRRI.ResultsThe GY reductions in RS trials compared to NS trials ranged from 79 to 99 %. Plant height (PH) was reduced and days to flowering (DTF) was delayed under RS. Eleven BC1F5 MRQ74 PLs with yield advantages of 1009 to 3473 kg ha−1 under RS and with yields equivalent to MRQ74 under NS trials were identified as promising drought tolerance PLs. Five best PLs, IR 98010-126-708-1-4, IR 98010-126-708-1-3, IR 98010-126-708-1-5, IR 99616-44-94-1-1, and IR 99616-44-94-1-2 with a yield advantage of more than 1000 kg ha−1 under RS and with yield potential equivalent to that of MRQ74 under NS were selected. The effect of three drought grain yield QTLs under RS in MRQ74 was validated. Under NS, PLs with two qDTY combinations (qDTY2.2 +qDTY12.1) performed better than PLs with other qDTY combinations, indicating the presence of a positive interaction between qDTY2.2 and qDTY12.1 in the MRQ74 background.ConclusionDrought tolerant MRQ74 PLs with a yield advantage of more than 1000 kg ha−1 under RS were developed. Differential yield advantages of different combinations of the qDTYs indicate a differential synergistic relationship among qDTYs.

Development of nutritious rice with high zinc/selenium and low cadmium in grains through QTL pyramiding

“…Many elite varieties have been successfully developed by pyramiding major QTLs or genes in rice recently (Shamsudin et al 2016; Zeng et al 2017; Das et al 2018; Feng et al 2018; Kumar et al 2018), indicating that rational design is a powerful tool to improve complex traits and create super‐varieties. GCC3 and GCC5 were two other QTLs identified for grain Cd concentration in our used population, but only GCC3 PA64s allele had a negative effect on Cd accumulation in grains (Table 1; Liu et al 2020).…”

Development of Drought Resistance in Rice

“…Several traditionally grown landraces such as KhaoDawk Mali, Azucena, Dular, Rayada, Bala, Apo, Nam Sagui 19, Nagina 22, AdaySel, Dehula, Moroberekan, Huma Wangi Lenggong, Siam Pilihan, ChianungSen Yu, Kashmir Basmati, MR142, FR13A, KDML 105, Azucena and Dular have great adaptability to survive in drought and their hidden genetic potential offers a better opportunity to improve drought tolerance in mega varieties (Pantuwan et al, 2002;Venuprasad et al, 2007;Henry et al, 2011;Vikram et al, 2012;Shamsudin et al,. 2016).…”

Penilaian Sifat Fisio-kimia dan Analisis Hedonik bagi Titisan Piramid Padi Tahan Kemarau Terpilih

“…Kajian oleh Shamsudin et al (2016aShamsudin et al ( , 2016b) berjaya menghasilkan beberapa PL dengan kelebihan hasil 0.5 tan sehektar atau lebih berbanding induk-induk penerima iaitu MR219 dan MRQ74 dalam tekanan kemarau di peringkat reproduktif dan tanpa kemarau melalui kaedah MAB langkah demi langkah. Tiga lokus ciri kuantitatif bagi hasil (qDTY) iaitu qDTY 2.2 , qDTY 3.1 dan qDTY 12.1 telah dipindahkan secara serentak ke dalam MR219 dan MRQ74 dan kehadiran kesemua qDTY pada PL disahkan menggunakan penanda mikrosatelit yang berkait rapat dengannya.…”

“…Penilaian dijalankan menggunakan reka bentuk rawak lengkap dengan tiga replikasi. PL yang dihasilkan mempunyai antara 83% hingga 98% dan antara 82% hingga 90% kandungan genom induk penerima bagi populasi MR219 dan MRQ74 dengan pemilihan dilakukan berdasarkan morfologi pokok dan bentuk bijian yang sama dengan induk penerima selain ketahanannya terhadap kemarau (Shamsudin et al 2016a(Shamsudin et al , 2016b. Kesemua genotip diambil daripada percubaan hasil lanjutan (AYT) pada musim utama 2016 di Stesen Padi, Tanaman Industri dan Florikultur (PTIF), Titi Serong, Perak.…”