Pyoverdine Analysis – From High-resolution MS/MS Fragmentation to Ion Mobility Measurements

Abstract

Microorganisms produce iron chelators called siderophores that are a rich source for drug discovery or plant protective agents. Pyoverdines are a class of siderophores from fluorescent Pseudomonas members and consist of different peptide chains specific to each bacterial species. The structural elucidation and characterization of pyoverdines require comprehensive analytical methods as bacterial extracts are complex mixtures. Here, we present a high-throughput UHPLC-MS/MS pipeline and the application of ion mobility spectrometry to facilitate research in the field of medicine and agriculture.

A Practical Toolkit for the Detection, Isolation, Quantification, and Characterization of Siderophores and Metallophores in Microorganisms

“…Although the determination of the exact mass of a pyoverdine together with its IEF pattern or iron uptake behavior provides sufficient information to unambiguously identify a pyoverdine, this requires the performance of at least two experiments. To avoid that, a comprehensive and easy to replicate universal high-throughput UHPLC–MS/MS pipeline was proposed to elucidate the peptide chain of pyoverdines and their derivatives in bacterial liquid cultures . To avoid the need for iron uptake or IEF experiments, the authors proposed the measurement of CCS values by IMS as an alternative identification marker …”

“…To avoid that, a comprehensive and easy to replicate universal high-throughput UHPLC–MS/MS pipeline was proposed to elucidate the peptide chain of pyoverdines and their derivatives in bacterial liquid cultures. 119 To avoid the need for iron uptake or IEF experiments, the authors proposed the measurement of CCS values by IMS as an alternative identification marker. 119 …”

“… 119 To avoid the need for iron uptake or IEF experiments, the authors proposed the measurement of CCS values by IMS as an alternative identification marker. 119 …”