Pyocin inhibition of Neisseria gonorrhoeae: mechanism of action

Morse, Stephen; Jones, Brian V.; Lysko, Paul G.

doi:10.1128/aac.18.3.416

Cited by 22 publications

(15 citation statements)

References 30 publications

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“…2B). This would not be surprising, since P. aeruginosa produces several extracellular antimicrobial molecules (many controlled by the pqsA-E operon) known to be important for lysis of S. aureus (11,17,26,27). The DMC coculture experiments were also supported by in vitro data, which showed that growth yields of P. aeruginosa were increased in iron-limited media when grown in coculture with S. aureus (Fig.…”

Section: Discussionsupporting

confidence: 69%

Staphylococcus aureus Serves as an Iron Source for Pseudomonas aeruginosa during In Vivo Coculture

et al. 2005

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Pseudomonas aeruginosa is a gram-negative opportunistic human pathogen often infecting the lungs of individuals with the heritable disease cystic fibrosis and the peritoneum of individuals undergoing continuous ambulatory peritoneal dialysis. Often these infections are not caused by colonization with P. aeruginosa alone but instead by a consortium of pathogenic bacteria. Little is known about growth and persistence of P. aeruginosa in vivo, and less is known about the impact of coinfecting bacteria on P. aeruginosa pathogenesis and physiology. In this study, a rat dialysis membrane peritoneal model was used to evaluate the in vivo transcriptome of P. aeruginosa in monoculture and in coculture with Staphylococcus aureus. Monoculture results indicate that approximately 5% of all P. aeruginosa genes are differentially regulated during growth in vivo compared to in vitro controls. Included in this analysis are genes important for iron acquisition and growth in low-oxygen environments. The presence of S. aureus caused decreased transcription of P. aeruginosa ironregulated genes during in vivo coculture, indicating that the presence of S. aureus increases usable iron for P. aeruginosa in this environment. We propose a model where P. aeruginosa lyses S. aureus and uses released iron for growth in low-iron environments.Pseudomonas aeruginosa is a gram-negative opportunistic human pathogen commonly found in water and soil. P. aeruginosa causes a number of chronic and acute infections and is noted for its inherent resistance to many clinically relevant antibiotics. Two of the most common infections caused by P. aeruginosa are chronic colonization of the lungs of individuals with the genetic disease cystic fibrosis (CF) (19) and peritonitis in individuals undergoing continuous ambulatory peritoneal dialysis (CAPD) (25). The lungs of CF patients are commonly colonized before the age of 8, and most individuals maintain these infections throughout their lifetimes. High infection rates are also associated with CAPD, which is often used to treat end-stage renal disease.P. aeruginosa physiology and gene expression during in vivo growth is largely unknown. Using in vivo expression technology (IVET), Wang et al. identified 19 P. aeruginosa genes inducible during growth in a neutropenic mouse (40). Although that study identified several new genes important for virulence in P. aeruginosa, it did not provide a comprehensive analysis of in vivo gene expression. Two recent studies using Pasteurella multocida and Borrelia burgdorferi have provided a more comprehensive view of in vivo bacterial gene expression by using DNA microarrays (3, 4, 33). These studies illustrate that a significant number of genes (2 to 8% of all the genes in the genomes) are differentially regulated in vivo, suggesting that the in vivo environment is distinct from normal in vitro culture conditions.Although evaluation of the transcriptomes of in vivo-grown bacteria provides a snapshot of transcription under monoculture growth conditions, it is clear that many in...

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Section: Discussionsupporting

confidence: 69%

Staphylococcus aureus Serves as an Iron Source for Pseudomonas aeruginosa during In Vivo Coculture

et al. 2005

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“…Serracin P of Serratia plymithicum J7 has antibacterial activity against Erwinia amylovora, Klebsiella pneumoniae, Serratia liquefaciens, Serratia marcescens and P. fluorescens (Jabrane et al 2002). R-type pyocins have been shown to kill some strains of Haemophilus, Neisseria and Campylobacter species (Morse et al 1980;Blackwell and Law 1981;Blackwell et al 1982). A recently reported PTLB, BceTMilo, has a broad host range and is active against many Burkholderia species and P. aeruginosa PAO1 (Yao et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Characterization of maltocin S16, a phage tail‐like bacteriocin with antibacterial activity againstStenotrophomonas maltophiliaandEscherichia coli

et al. 2019

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Aims To determine the characteristics of the novel phage tail‐like bacteriocin (PTLB) produced by Stenotrophomonas maltophilia S16. Methods and Results A screen to identify novel bacteriocins from a panel of 86 S. maltophilia strains was performed. We found that 62 tested S. maltophilia strains were sensitive to a PTLB, designated maltocin S16. In addition, 8 of 14 tested Escherichia coli strains were also susceptible to maltocin S16. Minimum inhibitory concentration determination confirmed that maltocin S16 had rather high bactericidal activity against both S. maltophilia and E. coli. Maltocin S16 was resistant to trypsin and proteinase K. Furthermore, lipopolysaccharide was found to be involved in the adsorption of maltocin S16. The gene cluster of maltocin S16 was consisted of 23 putative open reading frames. Phylogenetic analysis indicated that maltocin S16 represents a new class of PTLB in S. maltophilia. Conclusion Maltocin S16 was a novel PTLB with broad‐spectrum and high bactericidal activity against S. maltophilia and E. coli. Significance and Impact of the Study Stenotrophomonas maltophilia is a multidrug‐resistant opportunistic pathogen with increasing contributions to infectious disease morbidity and mortality. Maltocin S16 could be a promising alternative for antibiotics against S. maltophilia and E. coli infections.

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“…Contact with a single R-type bacteriocin particle can result in cell death, accounting for the potent bactericidal activity. The natural targets of R-type bacteriocins are typically other strains of the same bacterial species that produce the particles, although rare coincidental killing of other species by R-type pyocins has been noted (2,3,8,24,25). As a general rule, strains that produce these particles are resistant to their own bacteriocin.…”

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confidence: 99%

Novel High-Molecular-Weight, R-Type Bacteriocins of Clostridium difficile

et al. 2012

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c Clostridium difficile causes one of the leading nosocomial infections in developed countries, and therapeutic choices are limited. Some strains of C. difficile produce phage tail-like particles upon induction of the SOS response. These particles have bactericidal activity against other C. difficile strains and can therefore be classified as bacteriocins, similar to the R-type pyocins of Pseudomonas aeruginosa. These R-type bacteriocin particles, which have been purified from different strains, each have a different C. difficile-killing spectrum, with no one bacteriocin killing all C. difficile isolates tested. We have identified the genetic locus of these "diffocins" (open reading frames 1359 to 1376) and have found them to be common among the species. The entire diffocin genetic locus of more than 20 kb was cloned and expressed in Bacillus subtilis, and this resulted in production of bactericidal particles. One of the interesting features of these particles is a very large structural protein of ϳ200 kDa, the product of gene 1374. This large protein determines the killing spectrum of the particles and is likely the receptor-binding protein. Diffocins may provide an alternate bactericidal agent to prevent or treat infections and to decolonize individuals who are asymptomatic carriers.

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Pyocin inhibition of Neisseria gonorrhoeae: mechanism of action

Cited by 22 publications

References 30 publications

Staphylococcus aureus Serves as an Iron Source for Pseudomonas aeruginosa during In Vivo Coculture

Staphylococcus aureus Serves as an Iron Source for Pseudomonas aeruginosa during In Vivo Coculture

Characterization of maltocin S16, a phage tail‐like bacteriocin with antibacterial activity againstStenotrophomonas maltophiliaandEscherichia coli

Novel High-Molecular-Weight, R-Type Bacteriocins of Clostridium difficile

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