PyMiceTracking: An Open-Source Toolbox For Real-Time Behavioral Neuroscience Experiments

Menezes, Richardson Santiago Teles de; Miranda, Aron de; Maia, Helton

doi:10.1109/cvpr52688.2022.02079

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…Locomotion in the EPM and OF was analyzed by custom software (available on https://github.com/xarmison/proj-pca) (52). Each animal was evaluated once (independent observations for experimental factors: treatment and time).…”

Section: Analysis and Statisticsmentioning

confidence: 99%

Serotonergic psychedelic 5-MeO-DMT alters plasticity-related gene expression and generates anxiolytic effects in stressed mice

Nogueira,

Golbert,

Menezes

et al. 2023

Preprint

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Serotonergic psychedelics have potential therapeutic effects in treating anxiety and mood disorders, often after a single dose, and are suggested to have plasticity-inducing action. One lesser studied psychedelic, the 5-methoxy-N, N-dimethyltryptamine (5-MeO-DMT), is suggested to have anxiolytic effects yet a comprehensive mechanism of action is still lacking. Here, we investigated the effects of a single high-dose of the short-acting 5-MeO-DMT on gene expression from microdissected brain regions (anterior cingulate cortex - ACC; basolateral amygdala - BLA; ventral hippocampus CA1 region - vCA1 and dentate gyrus - DG) of naive and stressed mice. Specifically, we compared gene expression of Arc, Zif268, BDNF, CREB, mTORC1, NR2A, TRIP8b and NFkB in mice injected with 5-MeO-DMT or saline at different time points (1 hr, 5 hrs or 5 days prior). 5-MeO-DMT altered mRNA expression of immediate early genes Arc and ZiF268 in the ACC, BLA and vCA1, while only NR2A expression was altered after 5 hrs in the vCA1. We also found a long-term increase in TRIP8b, a gene related to the modulation of neuronal activity, in the vCA1 after 5 days. Behaviorally, 5-MeO-DMT treated mice showed mixed anxiolytic and anxiogenic effects in the elevated plus maze and open field test 24 hr or 5 days after treatment. However, pre-treated mice subjected to acute stress showed both lower corticosterone levels and robust anxiolytic effects of 5-MeO-DMT administration. Together, our findings provide insights into the molecular actions of 5-MeO-DMT in the brain related to anxiolytic effects of behavior.

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Section: Analysis and Statisticsmentioning

confidence: 99%