Pymetrozine is hydroxylated by CYP6CM1, a cytochrome P450 conferring neonicotinoid resistance in Bemisia tabaci

Abstract

The known cross-resistance between imidacloprid, other neonicotinoid insecticides and pymetrozine in B. tabaci is most likely conferred by the very same detoxification mechanism, i.e. a monooxygenase-based hydroxylation mechanism linked to the overexpression of CYP6CM1. These insecticide chemistries should not be alternated in whitefly resistance management strategies.

The function of two P450s, CYP9M10 and CYP6AA7, in the permethrin resistance of Culex quinquefasciatus

“…This co-infection strategy has been explored in studies of human CYP1A1 29 as well as insect P450s such as CYP9T2 in Ips pini 37 , CYP6BQ9 in Tribolium castaneum 30 , CYP6CM1 in Bemisia tabaci 32 and CYP6BQ23 in Meligethes aeneus 33 . In baculovirus-mediated expression systems, suitable MOI ratios for the gene-recombinant baculovirus co-infections are not only determined by the protein production and toxicity of the virus in the cells 38 , but also by the enzymatic catalytic activity, which depends on properly posttranslational modified proteins accumulating in the right ratio as a result of primary, secondary and, possibly, tertiary infections of both viruses 39, 40 .…”

Reversal of Insecticide Resistance in Florida Populations ofDiaphorina citri(Hemiptera: Liviidae)

“…Imidacloprid is a neonicotinoid and flupyradifurone is a butenolide. Data generated thus far suggest that cross-resistance is not likely between these 2 insecticides based on known mechanisms of resistance (Nauen et al 2013(Nauen et al , 2015. It remains to be demonstrated experimentally, but given the difference in chemical structure, it is unlikely the CYP4 P450 monooxygenases associated with imidacloprid exposure in D. citri will also be responsive to flupyradifurone (Tiwari et al 2011b;Killiny et al 2014).…”

“…Flupyradifurone targets the same receptor as neonicotinoids; however, the difference in structure between these insecticides may prevent cross resistance. Specifically, flupyradifurone is not metabolized by recombinant CYP6CM1, a P450 associated with resistance to imidacloprid in whiteflies (Karunker et al 2008;Nauen et al 2013Nauen et al , 2015. Further, no cross-resistance has been found to date between flupyradifurone and imidacloprid in several sap-sucking pests with high degrees of resistance to imidacloprid (Nauen et al 2015).…”

Suscetibilidade de adultos de Bemisia tabaci biótipo B a inseticidas

“…Todavia, resultados mais recentes demonstraram existir resistência cruzada a neonicotinoides e a pimetrozina em populações de B. tabaci (Nauen et al, 2013). Ensaios futuros devem prever a avaliação da pimetrozina no controle da mosca--branca sob condições diversas (casa de vegetação e campo) e sob diferente pressão de seleção da praga (densidade populacional), bem como investigar o risco de evolução da resistência nas populações da praga e o surgimento de sintomas de begomovirose após o surgimento de sintomas de begomovirose após o tratamento de plantas infestadas com moscas-brancas virulíferas.…”