Pyloric gastrin-producing cells and pyloric sphincter muscle cells are nuclear targets for 3H 1,25(OH)2 vitamin D3

Stumpf, Walter E.; Sar, Madhabananda; O'Brien, L. P.; Morin, Jacques

doi:10.1007/bf00492601

Cited by 16 publications

(5 citation statements)

References 11 publications

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“…Radioassays provided no evidence for the presence of vitamin D sites of action in the stomach. This contrasts with our autoradiographic demonstration of several distinct target cell populations in gastric glands, antrum, and pylorus (2,12).…”

Section: Stomachcontrasting

confidence: 53%

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Vitamin D and the digestive system

Stumpf

2008

Eur. J. Drug Metabol. Pharmacokinet.

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Target tissues of in vivo receptor binding and deposition of 1,25(OH)2 vitamin D3 and its oxygen analog OCT are reviewed in rats, mice, hamsters and zebra finch, identified with high-resolution microscopic autoradiography. Throughout the digestive system numerous sites with nuclear receptor binding of 3H-1,25(OH)2 vitamin D3 and 3H-OCT exist: in the oral region, epithelial cells of the oral cavity, tongue and gingiva, teeth odontoblast and ameloblast precursor pulp and stratum intermedium cells; in the parotid, submandibular and sublingual salivary glands, epithelial cells of striated ducts and granular convoluted tubules, intercalated ducts and acinar cells, as well as myoepithelial cells; in the stomach, neck mucous cells of gastric glands, endocrine cells of the antrum, and muscle cells of the pyloric sphincter; in the small and large intestine, absorptive and crypt epithelial cells; in the pancreas, predominantly islet B-cells. Perisinusoidal stellate (Ito) cells in the liver concentrate and retain variable amounts of radiolabeled compound in regions of their cytoplasm after administration of 3H-I,25(OH)2 vitamin D3 and 3H-25(OH) vitamin D3, probably sites of specific storage, similar to vitamin A. Submucosa in stomach and intestine also retain variable amounts of radiolabel, however unspecific with all compounds studied. In pilot studies with 3H-25(OH)2 vitamin D3 and 3H-24,25(OH)2 vitamin D3, no nuclear concentration was detectable. The reviewed data for vitamin D and its oxygen analogue OCT indicate genomic effects on multiple target tissues of the digestive system that involve cell proliferation and differentiation, endo- and exocrine secretion, digestion and absorption for maintaining optimal functions, with potentials for health prophylaxis and therapies.

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Section: Stomachcontrasting

confidence: 53%

“…The nuclear concentration in gastrin cells and other endocrine cells in the antrum (2,11,12) indicates vitamin D regulation of gastrin endocrine and paracrine secretion with secondary effects, e.g. on parietal cell HCL and chief cell pepsinogen secretion.…”

Section: Stomachmentioning

confidence: 99%

Vitamin D and the digestive system

Stumpf

2008

Eur. J. Drug Metabol. Pharmacokinet.

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“…When a vitamin deplete state exists, both 25 hydroxy and 1,25 dihydroxyvitamin D3 levels are low, the 1,25 being the last to reflect this deficit. As is now evident for vitamin D3, pineal hormone also affects functions of brain, pituitary, thyroid, adrenal, pancreatic islands, kidney, and many others (reviewed Relkin 1983;Stumpf 1988b). At this point, 1,25(OH)2 vitamin D 3 may feed back to inhibit dermal synthesis of the prohormone and deflect toward synthesis of inert by-products such as lumistrol or tachysterol (Holick 1985) and to increase melanin production in melanocytes.…”

Section: 25(oh)2 Vitamin D 3 (Soltriol)mentioning

confidence: 99%

Light, vitamin D and psychiatry

Stumpf

Privette

1989

Psychopharmacology

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This is a review and a prospectus of effects of vitamin D on the brain. Effects of sunlight and equivalent artificial light on physiological and behavioral processes are probably mediated, in large part, through the skin-vitamin D-endocrine system. Experimental evidence from our laboratory reveals sites of action and concomitant direct effects of 1,25(OH)2 vitamin D3 (soltriol) on brain, spinal cord, pituitary and other endocrine tissues. This appears relevant for the activation and modulation of mental and endocrine processes, particularly related to seasonal and daily biorhythms. Effects of sunlight and corresponding artificial light are likely to be mediated through direct actions of soltriol on brain and endocrine tissues that are independent of its effect on calcium levels. Those direct actions are receptor mediated and appear to be dose related as they depend on intensity of light and length of exposure, considering light (photons) as a drug. A role for soltriol, the steroid hormone of sunlight, in the etiology and helio- or phototherapy of affective disorders with cyclic seasonal onset (seasonal affective disorder) is discussed and the significance of research in the new frontier of vitamin D and brain relationships is noted.

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“…1989. Vitamin D receptors in female reproductive organs: ovary germinal epithelium, oviduct and uterus (66).…”

Section: Discoveries Related New Concepts and Paradigms Through The mentioning

confidence: 99%

Receptor localization of steroid hormones and drugs: discoveries through the use of thaw-mount and dry-mount autoradiography

Stumpf¹

1998

Braz J Med Biol Res

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The history of receptor autoradiography, its development and applications, testify to the utility of this histochemical technique for localizing radiolabeled hormones and drugs at cellular and subcellular sites of action in intact tissues. Localization of diffusible compounds has been a challenge that was met through the introduction of the "thaw-mount" and "dry-mount" autoradiographic techniques thirty years ago. With this cellular receptor autoradiography, used alone or combined with other histochemical techniques, sites of specific binding and deposition in vivo and in vitro have been characterized. Numerous discoveries, some reviewed in this article, provided information that led to new concepts and opened new areas of research. As an example, in recent years more than fifty target tissues for vitamin D have been specified, challenging the conventional view about the main biological role of vitamin D. The functions of most of these vitamin D target tissues are unrelated to the regulation of systemic calcium homeostasis, but pertain to the (seasonal) regulation of endo- and exocrine secretion, cell proliferation, reproduction, neural, immune and cardiovascular responses, and adaptation to stress. Receptor autoradiography with cellular resolution has become an indispensable tool in drug research and development, since information can be obtained that is difficult or impossible to gain otherwise.

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Pyloric gastrin-producing cells and pyloric sphincter muscle cells are nuclear targets for 3H 1,25(OH)2 vitamin D3

Cited by 16 publications

References 11 publications

Vitamin D and the digestive system

Vitamin D and the digestive system

Light, vitamin D and psychiatry

Receptor localization of steroid hormones and drugs: discoveries through the use of thaw-mount and dry-mount autoradiography

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