PYL8 ABA receptors of<i>Phoenix dactylifera</i>play a crucial role in response to abiotic stress and are stabilized by ABA (118)

García-Maquilón, Irene; Coego, Alberto; Lozano‐Juste, Jorge; Messerer, Maxim; Ollas, Carlos de; Julian, Jose; Ruiz-Partida, Rafael; Pizzio, Gastón A.; Belda-Palazón, Borja; Gómez-Cádenas, Aurelio; Mayer, Klaus; Geiger, Dietmar; Alquraishi, Saleh A.; Alrefaei, Abdulwahed Fahad; Ache, Peter; Hedrich, Rainer; Rodriguez, Pedro L.

doi:10.1101/2020.09.29.318170

Cited by 3 publications

(5 citation statements)

References 67 publications

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“…The PYL8 was reported to mediate ABA perception, and in turn ABA specifically stabilizes PYL8 and induces its accumulation in plant (Belda‐Palazon et al ., 2018; Garcia‐Maquilon et al ., 2021). Therefore, we examined whether exogenous ABA treatment would affect the interactions of IbPYL8 by Y2H and LCI assays.…”

Section: Resultsmentioning

confidence: 99%

“…AtPYL8 ‐overexpressing Arabidopsis plants were hypersensitive to ABA and exhibited high degrees of stomatal closure in response to ABA (Lim et al ., 2013). In date palm ( Phoenix dactylifera ), the PdPYL8‐like receptor Pd27 accumulated after ABA treatment, and Pd27 ‐overexpressing plants were more efficient than the WT in reducing transpiration under a negative soil water potential, leading to enhanced drought tolerance (Garcia‐Maquilon et al ., 2021). HvABI5 is involved in the ABA‐dependent drought response in barley (Collin et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

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The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

et al. 2022

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Drought limits crop development and yields. bHLH (basic helix-loop-helix) transcription factors play critical roles in regulating the drought response in many plants, but their roles in this process in sweet potato are unknown.Here, we report that two bHLH proteins, IbbHLH118 and IbbHLH66, play opposite roles in the ABA-mediated drought response in sweet potato. ABA treatment repressed IbbHLH118 expression but induced IbbHLH66 expression in the drought-tolerant sweet potato line Xushu55-2. Overexpressing IbbHLH118 reduced drought tolerance, whereas overexpressing IbbHLH66 enhanced drought tolerance, in sweet potato.IbbHLH118 directly binds to the E-boxes in the promoters of ABA-insensitive 5 (IbABI5), ABA-responsive element binding factor 2 (IbABF2) and tonoplast intrinsic protein 1 (IbTIP1) to suppress their transcription. IbbHLH118 forms homodimers with itself or heterodimers with IbbHLH66. Both of the IbbHLHs interact with the ABA receptor IbPYL8. ABA accumulates under drought stress, promoting the formation of the IbPYL8-IbbHLH66-IbbHLH118 complex. This complex interferes with IbbHLH118's repression of ABA-responsive genes, thereby activating ABA responses and enhancing drought tolerance.These findings shed light on the role of the IbPYL8-IbbHLH66-IbbHLH118 complex in the ABA-dependent drought response of sweet potato and identify candidate genes for developing elite crop varieties with enhanced drought tolerance.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

et al. 2022

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“…Among these, PYL8-LIKE 27 was the most highly expressed at the yellow-to-brown transition (Fig. S2; Garcia-Maquilon et al, 2021). The identity of the signal that regulates the timing of the natural increase in fruit pericarp ABA levels and how it is related to the onset of fruit ripening remains to be determined.…”

Section: Is Aba a Multi-tasking Hormone?mentioning

confidence: 99%

Abscisic acid plays a key role in the regulation of date palm fruit ripening

Elbar

Maytal

Imhonopi

et al. 2022

Preprint

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The date palm (Phoenix dactylifera L.) fruit is of major importance for the nutrition of broad populations in the world's desert strip, yet this crop is sorely understudied. Understanding the mechanisms regulating date fruit development and ripening is essential to customize this crop to the climatic change, which elaborates yield losses due to often too early occurring wet season. This study aimed to elucidate the mechanism regulating date fruit ripening. To that end, we followed the natural process of date fruit development and the effects of exogenous hormone application on fruit ripening in the elite cultivar 'Medjool'. The results of the current study indicate that the onset of fruit ripening occurred once the seed had reached maximum dry weight. From this point until fruit harvest, the pulp endogenous abscisic acid (ABA) turned up and its levels consistently increased. The final stage in fruit ripening, the yellow-to-brown transition, was preceded by an arrest of xylem-mediated water transport into the fruit. Exogenous ABA application enhanced fruit ripening when applied just prior to the green-to-yellow fruit color transition. Repeated ABA applications hastened all fruit ripening processes, resulting in a significantly earlier harvest. The emerging pivotal role of ABA in the regulation of date fruit ripening is thoroughly discussed.

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“…The response to abiotic stress is usually accompanied by hormone regulation [29,30]. Analysis of the hormone-related cis-acting elements in GhCNGCs showed that the number of ABA-responsive cis-acting elements (60) was the highest (Table S5). ABA plays an important role in the regulatory response to salt and drought stress [30][31][32].…”

Section: Analysis Of Cis-acting Elements In Ghcngcsmentioning

confidence: 99%

“…Studies have reported that ABA plays a crucial role in the response to abiotic stress and is a central regulator of the abiotic stress response [60,61], leading to significant changes in gene expression and adaptive physiological responses [34]. Salt stress can induce the ABA signaling regulatory mechanism, which plays an important role in the regulation of plant salt tolerance [62,63].…”

Section: Functional Analysis Of the Ghcngc32 And Ghcngc35 Genes Invol...mentioning

confidence: 99%

Systematic analysis of CNGCs in cotton and the positive role of GhCNGC32 and GhCNGC35 in salt tolerance

Yin

Chai

et al. 2022

BMC Genomics

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Background Cyclic nucleotide-gated ion channels (CNGCs) are calcium-permeable channels that participate in a variety of biological functions, such as signaling pathways, plant development, and environmental stress and stimulus responses. Nevertheless, there have been few studies on CNGC gene family in cotton. Results In this study, a total of 114 CNGC genes were identified from the genomes of 4 cotton species. These genes clustered into 5 main groups: I, II, III, IVa, and IVb. Gene structure and protein motif analysis showed that CNGCs on the same branch were highly conserved. In addition, collinearity analysis showed that the CNGC gene family had expanded mainly by whole-genome duplication (WGD). Promoter analysis of the GhCNGCs showed that there were a large number of cis-acting elements related to abscisic acid (ABA). Combination of transcriptome data and the results of quantitative RT–PCR (qRT–PCR) analysis revealed that some GhCNGC genes were induced in response to salt and drought stress and to exogenous ABA. Virus-induced gene silencing (VIGS) experiments showed that the silencing of the GhCNGC32 and GhCNGC35 genes decreased the salt tolerance of cotton plants (TRV:00). Specifically, physiological indexes showed that the malondialdehyde (MDA) content in gene-silenced plants (TRV:GhCNGC32 and TRV:GhCNGC35) increased significantly under salt stress but that the peroxidase (POD) activity decreased. After salt stress, the expression level of ABA-related genes increased significantly, indicating that salt stress can trigger the ABA signal regulatory mechanism. Conclusions we comprehensively analyzed CNGC genes in four cotton species, and found that GhCNGC32 and GhCNGC35 genes play an important role in cotton salt tolerance. These results laid a foundation for the subsequent study of the involvement of cotton CNGC genes in salt tolerance.

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PYL8 ABA receptors ofPhoenix dactyliferaplay a crucial role in response to abiotic stress and are stabilized by ABA (118)

Cited by 3 publications

References 67 publications

The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

The IbPYL8–IbbHLH66–IbbHLH118 complex mediates the abscisic acid‐dependent drought response in sweet potato

Abscisic acid plays a key role in the regulation of date palm fruit ripening

Systematic analysis of CNGCs in cotton and the positive role of GhCNGC32 and GhCNGC35 in salt tolerance

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