Pyk2 inhibition attenuates hypoxic-ischemic brain injury in neonatal mice

Zhu, Jie; Chu, Shifeng; Peng, Ye; Liu, Dandan; Chen, Chen; Jian, Wenxuan; Sun, Hong‐Shuo; Feng, Zhong‐Ping; Zhang, Zhao; Chen, Nai‐Hong

doi:10.1038/s41401-021-00694-5

Cited by 7 publications

(8 citation statements)

References 49 publications

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“…They can enhance antioxidant capacity and stabilize cell membranes during oxidative stress, thereby exerting a protective effect on biochemical markers [71,72]. Furthermore, curcumin is known to effectively prevent cellular damage caused by oxidative stress by reducing the production of reactive oxygen species [73].…”

Section: Discussionmentioning

confidence: 99%

“…The observed pathological lesions observed in the African catfish fed FFC in our study have been similarly reported in C. gariepinus [74], Oreochromis sp. [11,71], channel catfish [72], hybrid striped bass [73], and silver catfish (Rhamdia quelen) [74], following FFC or chloramphenicol treatments. Moreover, the increased vacuolization of hepatocytes observed after exposure to FFC is indicative of a degenerative process that could potentially cause metabolic damage, likely because of the medication [29,75].…”

Section: Discussionmentioning

confidence: 99%

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Dietary Nanocurcumin Impacts Blood Biochemical Parameters and Works Synergistically with Florfenicol in African Catfish Challenged with Aeromonas veronii

Mansour

Bakry

Alwaleed

et al. 2023

Fishes

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The present study investigated the effects of dietary supplementation of nanocurcumin (NCur = 150 ppm) in African catfish feed for 15 days along with two doses of florfenicol—a therapeutic dose (FFC1 = 10 mg/kg.BW) and a high dose (FFC2 = 30 mg/kg.BW). Serum biochemical parameters and histopathological changes in liver and kidney tissues were determined post-feeding. Additionally, fish were experimentally challenged with a virulent strain of Aeromonas veronii to explore the fish’s capacity to resist infections. Results indicated that the serum level of total protein was significantly elevated in the FFC2 + Ncur group compared with control, Ncur, and FFC2 groups. Total protein level was also higher in FFC1 + Ncur compared with that of the FFC1 group. Moreover, feeding NCur, FFC1 + Ncur, and FFC2 + Ncur resulted in a significant increase in globulin levels compared to control, FFC1, and FFC2-Fed groups. However, the albumin level was unaffected. Serum levels of AST, ALT, ALP, and urea significantly increased in FFC2-fed fish compared to controls indicating liver damage. The higher dosage of FFC2 induced histological alterations in the liver and kidney architecture, represented by a rupture of the central vein, cytoplasmic vacuolation, hepatocytes necrosis, and severe inflammation. Surprisingly, NCur inclusion improved most of the altered biochemical parameters and mitigated FFC-induced histological damage in the hepatic and renal tissues. Similarly, fish that were fed a combination of both FFC and Ncur showed a higher resistance when exposed to A. veronii infection, as the mortality rates were significantly lower than those of the control group. Our findings indicated that incorporating NCur into the diet can have multiple benefits for the sustainable aquaculture of African catfish. These advantages encompass mitigating drug-induced damage to the liver and kidney tissues, augmenting the therapeutic efficacy of FFC in a synergistic manner and providing protection against A. veronii infections.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dietary Nanocurcumin Impacts Blood Biochemical Parameters and Works Synergistically with Florfenicol in African Catfish Challenged with Aeromonas veronii

Mansour

Bakry

Alwaleed

et al. 2023

Fishes

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“…After deduplication, 4,630 articles were screened and 16 of them were retrieved as full texts. Subsequently, 6 studies were excluded for the following reasons: unclear description of randomization/eligibility cri-teria (n = 3) [31][32][33], no reporting of any the outcomes of interest (n = 1) [34], partial duplication of a trial already included (n = 1) [35], and initiation of hypothermia beyond 6 h after birth (n = 1) [36]. As a result, 10 studies [37][38][39][40][41][42][43][44][45][46] were included in the meta-analysis, comprising a total of 1,293 neonates.…”

Section: Study Selectionmentioning

confidence: 99%

Therapeutic Hypothermia for Neonatal Encephalopathy in Low- and Middle-Income Countries: A Meta-Analysis

2022

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Background: Perinatal asphyxia and hypoxic-ischemic encephalopathy (HIE) represent substantial sources of neonatal morbidity and mortality in low- and middle-income countries (LMICs), leading to high rates of adverse long-term neurological outcomes. Methods: A systematic review with meta-analysis of randomized controlled trials in LMICs was conducted. PubMed, Scopus, Web of Science, CENTRAL, ClinicalTrials.gov, and Google Scholar were searched from inception to August 20, 2021. The population of the study consisted of neonates with gestational age ≥34 weeks and HIE. The main endpoints were overall mortality and the composite outcome of death or severe disability. The certainty of evidence was evaluated with the GRADE approach. Results: Ten studies were included comprising 1,293 neonates. Some concerns of bias were raised due to the nonblinded nature of the intervention. The risk of death was similar between the two groups (risk ratio [RR]: 0.78, 95% confidence interval [CI]: 0.52–1.18). No significant differences were observed in the composite outcome of death or severe disability between the two groups (RR: 0.78, 95% CI: 0.56–1.10, very low quality of evidence). Furthermore, no significant differences were observed in the endpoints of sepsis, shock, acute kidney injury, major arrhythmia, and length of hospital stay. Therapeutic hypothermia was associated with significantly higher risk of thrombocytopenia (RR: 2.13, 95% CI: 1.34–3.38) and clinically significant hemorrhage (RR: 1.57, 95% CI: 1.25–1.97). Conclusion: Therapeutic hypothermia probably results in little to no difference in clinical outcomes among neonates with HIE in LMICs. Further large-scale research targeting proper patient selection is needed to elucidate the utility of therapeutic hypothermia in resource-limited settings. Protocol Registration: The protocol of the study has been prospectively registered by Prospero, CRD42021272284.

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Section: Introductionmentioning

confidence: 99%

“…It has a certain relationship with the change of intracellular calcium ion concentration and can be activated through a calcium-dependent mechanism and participate in the downstream signal transmission of Ca 2+ [37]. Phosphorylation of Pyk2 is increased in ischemic brain injury, and phosphorylation of Pyk2 promotes brain injuryinduced neuroinflammation [38]. This review will discuss the effects of Fyn and its downstream signaling pathways on ischemia-reperfusion injury Mediators of Inflammation and its related mechanisms and provide therapeutic strategies for alleviating ischemia-reperfusion injury and improving the prognosis of ischemic stroke.…”

Section: Introductionmentioning

confidence: 99%

Fyn Signaling in Ischemia-Reperfusion Injury: Potential and Therapeutic Implications

Tang

et al. 2022

Mediators of Inflammation

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Ischemic stroke caused by arterial occlusion is the most common type of stroke and is one of the leading causes of disability and death, with the incidence increasing each year. Fyn is a nonreceptor tyrosine kinase belonging to the Src family of kinases (SFKs), which is related to many normal and pathological processes of the nervous system, including neurodevelopment and disease progression. In recent years, more and more evidence suggests that Fyn may be closely related to cerebral ischemia-reperfusion, including energy metabolism disorders, excitatory neurotoxicity, intracellular calcium homeostasis, free radical production, and the activation of apoptotic genes. This paper reviews the role of Fyn in the pathological process of cerebral ischemia-reperfusion, including neuroexcitotoxicity and neuroinflammation, to explore how Fyn affects specific signal cascades and leads to cerebral ischemia-reperfusion injury. In addition, Fyn also promotes the production of superoxide and endogenous NO, so as to quickly react to produce peroxynitrite, which may also mediate cerebral ischemia-reperfusion injury, which is discussed in this paper. Finally, we revealed the treatment methods related to Fyn inhibitors and discussed its potential as a clinical treatment for ischemic stroke.

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Pyk2 inhibition attenuates hypoxic-ischemic brain injury in neonatal mice

Cited by 7 publications

References 49 publications

Dietary Nanocurcumin Impacts Blood Biochemical Parameters and Works Synergistically with Florfenicol in African Catfish Challenged with Aeromonas veronii

Dietary Nanocurcumin Impacts Blood Biochemical Parameters and Works Synergistically with Florfenicol in African Catfish Challenged with Aeromonas veronii

Therapeutic Hypothermia for Neonatal Encephalopathy in Low- and Middle-Income Countries: A Meta-Analysis

Fyn Signaling in Ischemia-Reperfusion Injury: Potential and Therapeutic Implications

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