Pygidial glands of the blue ground beetle Carabus intricatus: chemical composition of the secretion and its antimicrobial activity

Vesović, Nikola; Nenadić, Marija; Soković, Marina; Ćirić, Ana; Vujisić, Ljubodrag; Todosijević, Marina; Stevanović, Nataša; Perić-Mataruga, Vesna; Ilijin, Larisa; Ćurčić, Srećko

doi:10.1007/s00114-022-01790-0

Cited by 4 publications

(22 citation statements)

References 49 publications

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“…EI mass spectra (70 eV) were acquired in the range of 35-550 m/z. For all the analyses, the ion source temperature was 230°C and the quadrupole temperature was 150°C (Vesović et al, 2022). Mass spectral deconvolution and extraction, as well as a library search were performed using NIST Automated Mass Spectral Deconvolution and Identification System (AMDIS) ver.…”

Section: Chemical Extraction and Chemical Analyses Of The Secretionsmentioning

confidence: 99%

“…The recorded percentage variation of methacrylic acid is considered usual in the defensive secretions of ground beetles. Within Carabini, methacrylic acid is by far the most abundant compound (Schildknecht et al, 1964(Schildknecht et al, , 1968Lečić et al, 2014;Vesović et al, 2017Vesović et al, , 2020Vesović et al, , 2022. Tiglic acid is typically present along with methacrylic acid in the pygidial gland secretions of ground beetles (Giglio et al, 2021).…”

Section: Chemical Surveymentioning

confidence: 99%

“…It is fairly common for tiglic acid to be more abundant than angelic acid. The opposite case has been found to occur only in three taxa of Carabini [Carabus (Chaetocarabus) intricatus Linnaeus, 1760, C. (C.) ulrichii rhilensis and C. (Megodontus) violaceus Linnaeus, 1758] ( Lečić et al, 2014;Vesović et al, 2020Vesović et al, , 2022.…”

Section: Chemical Surveymentioning

confidence: 99%

“…The occurrence of multi-compound defensive secretions creates the possibility of variations. To date, a limited number of studies indicate the existence of alterations to defensive secretions caused by various ecological factors (Wheeler et al, 1970;Holliday et al, 2012Holliday et al, , 2016Vesović et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, the biological activity of secretions in Coleoptera has been increasingly investigated (Barnes et al, 2010;Nenadić et al, 2016aNenadić et al, ,b, 2017aNenadić et al, ,b, 2018Nenadić, 2019;Dimkić et al, 2020;Vesović et al, 2022). This is mainly due to the fact that compounds with antimicrobial properties could occur in their secretions, which probably contributes to the ecological role of these natural products (Nenadić et al, 2017a;Dimkić et al, 2020;Pu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The chemical composition of the secretions, their antibacterial activity, and the pygidial gland morphology of selected European Carabini ground beetles (Coleoptera: Carabidae)

et al. 2023

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The pygidial glands of carabids produce strong-smelling vapours. In this study, we examined the chemical composition of the gland secretions and the structure of the glands in five species of Carabini ground beetles (one species from the subtribe Calosomatina and four species from the subtribe Carabina): Calosoma (Calosoma) maderae (Fabricius, 1775), Carabus (Carabus) granulatus Linnaeus, 1758, C. (Limnocarabus) clathratus Linnaeus, 1760, C. (Carabus) ulrichii Germar, 1823, and C. (Procerus) gigas Creutzer, 1799. Additionally, we tested the antibacterial potential of the pygidial gland secretions of the two latter species against 11 bacterial strains. In order to detect the chemical content of the secretions, we used gas chromatography–mass spectrometry (GC–MS). The secretion extracts were applied against selected strains of medically important bacteria. We used bright-field microscopy to examine the morphology of the glands. We discovered a total of 11 chemical compounds in the pygidial gland extracts of the ground beetles we analysed. Ten of these compounds were identified as seven carboxylic acids, two hydrocarbons, and one aromatic aldehyde, while one chemical remained unidentified. Most of the components were isolated from the secretion of C. (L.) clathratus (nine), while the lowest number of compounds was found in C. (P.) gigas (two). Methacrylic acid was the most dominant compound by percentage in all five species, while angelic acid was also detected in all samples. As expected, salicylaldehyde was exclusively found in the species of the genus Calosoma Weber, 1801. The secretion of C. (P.) gigas was shown to achieve the highest level of antibacterial activity against Pseudomonas aeruginosa, Salmonella enterica, and S. typhimurium (even the same level as the positive control streptomycin), while the secretion of C. (C.) ulrichii achieved the highest antimicrobial potential against Staphylococcus epidermidis, S. aureus, Listeria monocytogenes, and Bacillus cereus. The most noticeable difference in the structure of the glands between the two genera is that the reservoir in Calosoma is more significantly narrowed as it leads into the efferent duct, compared to that of Carabus.

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Section: Chemical Extraction and Chemical Analyses Of The Secretionsmentioning

confidence: 99%