pXO16 from Bacillus thuringiensis serovar israelensis: Almost 350 kb of terra incognita

Makart, Lionel; Gillis, Annika; Mahillon, Jacques

doi:10.1016/j.plasmid.2015.03.002

Cited by 15 publications

(23 citation statements)

References 43 publications

(41 reference statements)

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“…In this regard, pXO16 is responsible for a remarkable aggregation phenomenon between donor and recipient cells that has been associated with conjugative transfer of plasmids . Analysis of pXO16 sequence has highlighted a potential region responsible for this aggregation phenotype (Makart et al, 2015). This 27-kb region mostly encodes proteins with putative surface-associated functions, including two very large related proteins with sizes of 3526 and 2558 residues, both containing 'collagen-binding protein-like B-type' domains and a C-terminal cell wall anchoring LPXTG motif.…”

Section: Pxo16 Aggregation Promotes Its Own Conjugative Transfermentioning

confidence: 99%

“…Interestingly, these LPXTG motives could be processed by a putative sortase, also encoded in this region. Additionally, four genes coding for proteins annotated as peptidoglycan-linked, glycoside hydrolase, muramidase and S-layer domain containing are also present (Makart et al, 2015).…”

Section: Pxo16 Aggregation Promotes Its Own Conjugative Transfermentioning

confidence: 99%

“…In an effort to identify the genes that could be involved in the conjugation mechanism of pXO16, a CDS coding for a putative DNA segregation ATPase FtsK/SpOIIIE appeared to be the sole candidate available in its sequence (Makart et al, 2015). In the Clostridium perfringens plasmid pCW3, the FtsK/SpOIIIE homologue TcpA fulfils the role of a coupling protein in a unique conjugation system related to T4SSs, hence transferring single stranded DNA (ssDNA) (Wisniewski and Rood, 2017).…”

Section: Pxo16 Is Transferred As Ssdnamentioning

confidence: 99%

“…At first, no obvious homolog related to T4SS could be clearly identified in pXO16 sequence by basic protein homology searches (Makart et al, 2015). Combined with its peculiar mobilization capabilities, it was thus likely that pXO16 encoded an unforeseen conjugation system.…”

Section: Introductionmentioning

confidence: 99%

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A novel T4SS‐mediated DNA transfer used by pXO16, a conjugative plasmid from Bacillus thuringiensis serovar israelensis

Makart

Gillis

Hinnekens

et al. 2018

Environmental Microbiology

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The entomopathogenic Bacillus thuringiensis serovar israelensis displays peculiar conjugative transfer capabilities, accounted for by the large conjugative plasmid pXO16 (350 kb). The efficient and fast conjugative transfers are accompanied by a macroscopic aggregation of bacterial partners. Moreover, pXO16 has proven capable of effective mobilization and the retro-transfer of both mobilizable and 'non-mobilizable' plasmids. In this work, the aggregation phenomenon is shown to promote pXO16 transfer while not being mandatory for transfer. Transfer of pXO16 to B. thuringiensis recipient strains that do not display aggregation is observed as well, hence enlarging the previously defined host range. The use of variant calling analysis of transconjugants allowed for observation of up to 791 kb chromosomal regions mobilization. Previous analysis of pXO16 did not reveal any Type IV Secretion System (T4SS) homologs, which suggested the presence of an unusual conjugative system. A FtsK/SpOIIIE ATPase gene proved here to be necessary for conjugative transfer. Additionally, the analysis of natural restriction-modification systems in both conjugative partners gave credit to a ssDNA transfer mechanism. A 'transfer israelensis plasmid' (tip) region containing this ATPase gene was shown to code for other potential T4SS proteins, illustrating a conjugative system distantly related to the other known Gram-positive T4SSs.

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Section: Pxo16 Aggregation Promotes Its Own Conjugative Transfermentioning

confidence: 99%

Section: Pxo16 Aggregation Promotes Its Own Conjugative Transfermentioning

confidence: 99%

Section: Pxo16 Is Transferred As Ssdnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel T4SS‐mediated DNA transfer used by pXO16, a conjugative plasmid from Bacillus thuringiensis serovar israelensis

Makart

Gillis

Hinnekens

et al. 2018

Environmental Microbiology

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“…Sequencing revealed a candidate plasmid, designated pBTHD789-1, 350 kb in size [17]. The obvious difference in plasmid sizes was not explained, but was finally experimentally resolved in a recent paper that identifies the 350 kb pBTHD789-1 as pXO16 [21]. This identification was based on direct sequencing of pXO16 parts adjacent to the Tn5401 transposon, used for plasmid labeling, and also on sequencing of several PCR amplicons from the pXO16 transferred into a plasmidless strain.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of extrachromosomal elements in Bacillus thuringiensis subsp. israelensis

Bolotin

Gillis

Sanchis

et al. 2017

Research in Microbiology

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Bacillus thuringiensis subsp. israelensis is one of the most important microorganisms used against mosquitoes. It was intensively studied following its discovery and became a model bacterium of the B. thuringiensis species. Those studies focused on toxin genes, aggregation-associated conjugation, linear genome phages, etc. Recent announcements of genomic sequences of different strains have not been explicitly related to the biological properties studied. We report data on plasmid content analysis of four strains using ultra-high-throughput sequencing. The strains were commercial product isolates, with their putative ancestor and type B. thuringiensis subsp. israelensis strain sequenced earlier. The assembled contigs corresponding to published and novel data were assigned to plasmids described earlier in B. thuringiensis subsp. israelensis and other B. thuringiensis strains. A new 360 kb plasmid was identified, encoding multiple transporters, also found in most of the earlier sequenced strains. Our genomic data show the presence of two toxin-coding plasmids of 128 and 100 kb instead of the reported 225 kb plasmid, a co-integrate of the former two. In two of the sequenced strains, only a 100 kb plasmid was present. Some heterogeneity exists in the small plasmid content and structure between strains. These data support the perception of active plasmid exchange among B. thuringiensis subsp. israelensis strains in nature.

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Characterization and Whole Genome Sequencing of AR23, a Highly Toxic Bacillus thuringiensis Strain Isolated from Lebanese Soil

et al. 2019

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pXO16 from Bacillus thuringiensis serovar israelensis: Almost 350 kb of terra incognita

Cited by 15 publications

References 43 publications

A novel T4SS‐mediated DNA transfer used by pXO16, a conjugative plasmid from Bacillus thuringiensis serovar israelensis

A novel T4SS‐mediated DNA transfer used by pXO16, a conjugative plasmid from Bacillus thuringiensis serovar israelensis

Comparative genomics of extrachromosomal elements in Bacillus thuringiensis subsp. israelensis

Characterization and Whole Genome Sequencing of AR23, a Highly Toxic Bacillus thuringiensis Strain Isolated from Lebanese Soil

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