Pvuii Genetic Polymorphism of Low Density Lipoprotein Receptorin Human Immunodeficiency Virus Type 1-Infected Patients: Possible Association with Dyslipidemia

Almeida, Elaine Regina Delicato de; Simão, Andréa Name Colado; HelenaKaminamiMorimoto,; Kallaur, Ana Paula; Flauzino, Tamires; Alfieri, Daniela Frizon; Oliveira, João; Ueda, LuisToshio; Lozovoy, Marcel Alysson Batisti; Dichi, Isaías; Watanabe, Maria AngelicaEhara; Reiche, Edna Maria Vissoci

doi:10.4172/2155-6113.1000362

Cited by 3 publications

(3 citation statements)

References 34 publications

(54 reference statements)

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“…Low-density lipoprotein receptor (LDLR), positioned on chromosome 19p13.2 plays a significant role in lipoprotein metabolism by mediating the uptake of cholesterol through the binding and subsequent cellular uptake of apolipoprotein-E and B-constituting lipoproteins. Mutations have been detected in different domains of the LDLR which have a distinct effect on LDLR structure and function [17,18]. ATP-binding cassette A1(ABCA1) plays a critical role in the reverse cholesterol transport system.…”

Section: Introductionmentioning

confidence: 99%

Association of single nucleotide polymorphisms with dyslipidemia in antiretroviral exposed HIV patients in a Ghanaian population: A case-control study

et al. 2020

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Dyslipidemia is a potential complication of long-term usage of antiretroviral therapy (ART) and also known to be associated with genetic factors. The host genetic variants associated with dyslipidemia in HIV patients on ART in Ghana have not been fully explored. The study constituted a total of 289 HIV-infected patients on stable ART for at least a year. Fasting blood was collected into EDTA tube for lipids measurement. Lipid profiles were used to define dyslipidemia based on the NCEP-ATP III criteria. HIV-infected subjects were categorized into two groups; those with dyslipidemia (cases) (n = 90; 31.1%) and without dyslipidemia (controls)(n = 199; 68.9%). Four candidate single nucleotide polymorphism (SNP) genes (ABCA1-rs2066714, LDLR-rs6511720, APOA5-rs662799 and DSCAML1-rs10892151) were determined. Genotyping was performed on isolated genomic DNA of study participants using PCR followed by a multiplex ligation detection reaction (LDR). The percentage of the population who had the rare homozygote alleles for rs6511720 (T/T), rs2066714 (G/G), rs10892151 (T/T) and rs662799 (G/G) among case subjects were 5.5%, 14.4%, 6.6% and 10.0% whiles 2.0% 9.1%, 6.5% and 4.0% were observed among control subjects. There were statistically significant differences in the genotypic prevalence of APOA5 (p = 0.0357) and LDLR polymorphisms (p = 0.0387) between case and control subjects. Compared to the AA genotype of the APOA5 polymorphisms, individuals with the rare homozygote genotype [aOR = 2.38, 95%CI(1.06-6.54), p = 0.004] were significantly associated with an increased likelihood of developing dyslipidemia after controlling for age, gender, treatment duration, CD4 counts and BMI. Moreover, individuals with the rare homozygous genotype of ABCA1 (G/G) [aOR = 10.7(1.3-88.7), p = 0.0280] and LDLR (rs6511720) G>T [aOR = 61.2(7.6-493.4), p<0.0001) were more likely to have high levels of total cholesterol levels. Our data accentuate the presence of SNPs in four candidate genes

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Section: Introductionmentioning

confidence: 99%

Association of single nucleotide polymorphisms with dyslipidemia in antiretroviral exposed HIV patients in a Ghanaian population: A case-control study

et al. 2020

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“…Особенность медико-биологических экспериментов на мышах, крысах и кроликах, проводимых по стандарту доклинических исследований, заключается в том, что стандарт не включает тепловизорный мониторинг динамики температуры исследуемых лекарств и тел животных при их взаимодействии, равно как и температуры пищи, питьевой воды, воздуха и окружающих предметов в помещениях, где осуществляется экспериментальная лекарственная терапия [11,12]. В таких условиях вряд ли возможно сохранение температуры всех перечисленных объектов в пределах 36,5-37,5 °C на протяжении многих часов и дней курсовой лекарственной терапии.…”

Section: эксперименты на мышах крысах и кроликах что мы не учитываем ...unclassified

“…Сообщается, что около 95 % таких исследований было проведено на крысах и мышах. Однако не грызуны, а собаки, свиньи и обезьяны обладают свойствами, делающими их незаменимыми для исследования болезней человека и фармакологической активности лекарств [12][13][14]. Тем не менее число этих животных составило менее 1 % от всех теплокровных животных, использованных в биомедицинских исследованиях [15][16][17].…”

Section: Introductionunclassified

Aquarium fish and temperature neuropharmacology: Update

Urakov,

Fisher,

Lebedev

et al. 2024

Psychopharmacology & biological narcology

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It is shown that at the end of the 20th century in Russia studies of temperature dependence of specific pharmacological activity of antihypoxants and some neurotropic drugs under normo- and hypothermia of various biological objects in experimental and clinical conditions were started. At the beginning of the 21st century, an original biological model of acute hypoxia in aquarium fish was developed. This model made it possible to start the study of fish resistance to hypoxia, to screen biologically active substances and to evaluate the activity of antihypoxants under normo- and hypothermia. The first results of using this model allowed to conclude that the dynamics of motor activity of fish under conditions of acute hypoxia is similar to the dynamics of motor activity of fetuses inside the uterus under diagnostic intrauterine hypoxia created by voluntary apnea in a pregnant woman. It was found that the value of the duration of the period of immobile state of fish and fetuses under hypoxia is directly proportional to the value of their resistance to hypoxia. It was found that the reduction in conditions of acute hypoxia water temperature with floating fish in it on 10 °C lengthens the duration of the period of immobile state of fish and the period of preservation of their viability more than 2 times, and the preliminary introduction of hydrogen peroxide in the water in the therapeutic dose in combination with hypothermia lengthens the corresponding periods by 4 times. It has also been found that such neurotropic drugs as ethyl alcohol, local and general anesthetics will completely eliminate the pain syndrome that develops during local hypothermia in normal. In addition, it has been shown that immersing the hands of people under alcohol intoxication and/or surgical anesthesia in water with melting ice for 2 minutes eliminates the feeling of pain in the hands and accelerates the development of subsequent hyperemia in the skin of the hands by 2 times compared to the norm.

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Association of Single Nucleotide Polymorphisms with Dyslipidemia in Antiretroviral Exposed HIV Patients in a Ghanaian population

Obirikorang

Acheampong

Quaye

et al. 2019

Preprint

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Dyslipidemia is a potential complication of long-term usage of antiretroviral therapy (ART) and also known to be associated with genetic factors. The host genetic variants associated with dyslipidemia in HIV patients on ART in Ghana have not been fully explored. The study constituted a total of 289 HIV-infected patients on stable ART for at least a year and 85 aged matched apparently healthy control subjects with no history of HIV and dyslipidemia. Fasting blood was collected into EDTA tube for lipids measurements. Lipid profiles were determined as a measure of dyslipidemia. HIV-infected patients were categorized into two groups; those with dyslipidemia(HIV-Dys+) (n=90; 31.1%) and without dyslipidemia (n=199; 68.9%) based on the NCEP-ATP III criteria. Four candidate single nucleotide polymorphisms (SNPs) genes (ABCA1-rs2066714, LDLR-rs6511720, APOA5-rs662799 and DSCAML1-rs10892151) were determined. Genotyping was performed on isolated genomic DNA of study participants using PCR followed by a multiplex Ligation Detection Reaction (LDR). The percentage of the population who had the rare homozygote alleles for rs6511720 (T/T), rs2066714 (G/G), and rs10892151 (T/T) and rs662799 (G/G) among HIV+Dys+ subjects were 5.5%, 14.4%, 6.6% and 10.0%; 2.0% 9.1%, 6.5% and 4.0% among HIV+Dys- subjects while 3.5%, 4.7%, 4.7% and 2.4% were observed in HIV-Dys- subjects. Statistically significant difference in genotypic prevalence of APOA5 polymorphisms was observed among different groups (p=0.0196). Compared to the AA genotype of the APOA5 polymorphisms, individuals with the rare homozygote genotype [aOR =4.01, 95%CI(1.57-22.39), p=0.004] were significantly more likely to develop dyslipidemia after controlling for age, gender, treatment duration and CD4 counts among the HIV+Dys+ subjects. There was also a significant associated between GG genotype of ABCA1 and dyslipidemia [aOR =3.29, 95% (1.08 -12.43); p=0.042] . Individuals with the rare homozygote variant (GG) of APOA5 (rs662799) were significantly associated with increased likelihood of developing dyslipidemia [OR =2.24, 95% CI (1.20 -6.83); p=0.0370] holding other variables constant in the HIV+Dys- subjects. Our data accentuate the presence of SNPs in four candidate genes and its association with dyslipidemia among HIV patients exposed to ART in the Ghanaian population especially variants in APOA5-rs662799 ABCA1-rs2066714 respectively. These findings provide baseline information that necessitates a pre-symptomatic strategy for monitoring dyslipidemia in ART-treated HIV patients. There is a need for longitudinal studies to validate a comprehensive number of SNPs and its association with dyslipidemia.

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Pvuii Genetic Polymorphism of Low Density Lipoprotein Receptorin Human Immunodeficiency Virus Type 1-Infected Patients: Possible Association with Dyslipidemia

Cited by 3 publications

References 34 publications

Association of single nucleotide polymorphisms with dyslipidemia in antiretroviral exposed HIV patients in a Ghanaian population: A case-control study

Association of single nucleotide polymorphisms with dyslipidemia in antiretroviral exposed HIV patients in a Ghanaian population: A case-control study

Aquarium fish and temperature neuropharmacology: Update

Association of Single Nucleotide Polymorphisms with Dyslipidemia in Antiretroviral Exposed HIV Patients in a Ghanaian population

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