PVPylated Poly(alanine) as a New Thermogelling Polymer

Han, Jin Ok; Joo, Min Kyung; Jang, Ji Hye; Park, Min Hee; Jeong, Byeongmoon

doi:10.1021/ma901089s

Cited by 18 publications

(16 citation statements)

References 45 publications

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“…A new thermogelling poly(N-vinyl pyrrolidone)-PA (PVP-PA) has been reported. [63] Aqueous solutions of the polymers showed sol-to-gel transitions. Gel formation was attributed to hydrophobic association and formation of a b-sheet structure.…”

Section: Polypeptidesmentioning

confidence: 99%

Injectable Biodegradable Hydrogels

Nguyen

Lee

2010

Macromolecular Bioscience

416

343

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Injectable biodegradable copolymer hydrogels, which exhibit a sol–gel phase transition in response to external stimuli, such as temperature changes or both pH and temperature (pH/temperature) alterations, have found a number of uses in biomedical and pharmaceutical applications, such as drug delivery, cell growth, and tissue engineering. These hydrogels can be used in simple pharmaceutical formulations that can be prepared by mixing the hydrogel with drugs, proteins, or cells. Such formulations are administered in a straightforward manner, through site‐specific control of release behavior, and the hydrogels are compatible with biological systems. This review will provide a summary of recent progress in biodegradable temperature‐sensitive polymers including polyesters, polyphosphazenes, polypeptides, and chitosan, and pH/temperature‐sensitive polymers such as sulfamethazine‐, poly(β‐amino ester)‐, poly(amino urethane)‐, and poly(amidoamine)‐based polymers. The advantages of pH/temperature‐sensitive polymers over simple temperature‐sensitive polymers are also discussed. A perspective on the future of injectable biodegradable hydrogels is offered.magnified image

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Section: Polypeptidesmentioning

confidence: 99%

Injectable Biodegradable Hydrogels

Nguyen

Lee

2010

Macromolecular Bioscience

416

343

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“…The hydrogels were stable in phosphate buffer but degraded quickly in the presence of enzymes. A new thermogelling poly( N ‐vinyl pyrrolidone)‐PA (PVP‐PA) has been reported 63. Aqueous solutions of the polymers showed sol‐to‐gel transitions.…”

Section: Injectable Biodegradable Block Copolymer Hydrogelsmentioning

confidence: 99%

Effects of arginine on heat‐induced aggregation of concentrated protein solutions

Shah

Shaikh

Peng

et al. 2011

Biotechnology Progress

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Arginine is one of the commonly used additives to enhance refolding yield of proteins, to suppress aggregation of proteins, and to increase solubility of proteins, and yet the molecular interactions that contribute to the role of arginine are unclear. Here, we present experiments, using bovine serum albumin (BSA), lysozyme (LYZ), and β-lactoglobulin (BLG) as model proteins, to show that arginine can enhance heat-induced aggregation of concentrated protein solutions, contrary to the conventional belief that arginine is a universal suppressor of aggregation. Results show that the enhancement in aggregation is caused only for BSA and BLG, but not for LYZ, indicating that arginine's preferential interactions with certain residues over others could determine the effect of the additive on aggregation. We use this previously unrecognized behavior of arginine, in combination with density functional theory calculations, to identify the molecular-level interactions of arginine with various residues that determine arginine's role as an enhancer or suppressor of aggregation of proteins. The experimental and computational results suggest that the guanidinium group of arginine promotes aggregation through the hydrogen-bond-based bridging interactions with the acidic residues of a protein, whereas the binding of the guanidinium group to aromatic residues (aggregation-prone) contributes to the stability and solubilization of the proteins. The approach, we describe here, can be used to select suitable additives to stabilize a protein solution at high concentrations based on an analysis of the amino acid content of the protein.

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“…Great efforts have been devoted to design thermosensitive micelles due to their scientific and industrial importance . Without a doubt, the most widely adopted strategy is to synthesize copolymers containing thermosensitive polymers such as polymers with lower critical solution temperature (LCST) or some biomacromolecules . Thus, there would be a temperature‐induced conformational change in these thermosensitive polymers including coil to globule transition in the synthetic polymers or a transition between different secondary structures in most biomacromolecules and polypeptides.…”

Section: Introductionmentioning

confidence: 99%

Gradient Structure‐Induced Temperature Responsiveness in Styrene/Methyl Methacrylate Gradient Copolymers Micelles

Zheng

Huang

2013

Macromol. Rapid Commun.

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In this work, micelles are formed by gradient copolymer of styrene and methyl methacrylate in acetone-water mixture and their temperature responsiveness is investigated in a narrow range near room temperature. Three different kinds of structural transitions could be induced by temperature: unimers to micelle transition, shrinkage/stretching of micelles, and morphological transition from spherical micelles to vesicles. In addition, a model analysis on the interface of gradient copolymer micelle is made to better understand these phenomena. It is found that both position and composition of the interface could alter in response to the change in temperature. According to the experiments and model analysis, it is proposed that temperature responsiveness might be an intrinsic and universal property of gradient copolymer micelles, which only originates from the gradient structure.

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PVPylated Poly(alanine) as a New Thermogelling Polymer

Cited by 18 publications

References 45 publications

Injectable Biodegradable Hydrogels

Injectable Biodegradable Hydrogels

Effects of arginine on heat‐induced aggregation of concentrated protein solutions

Gradient Structure‐Induced Temperature Responsiveness in Styrene/Methyl Methacrylate Gradient Copolymers Micelles

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