PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production

Parascandola, Palma; Branduardi, Paola; Alteriis, Elisabetta de

doi:10.1016/j.enzmictec.2005.06.004

Cited by 27 publications

(22 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The storage stability, after 6 weeks, of the PVA (10%)-alginate (1%) beads remains constant, corresponding to 90% of residual activity. In contrast to biopolymers, PVA hydrogels are hardly biodegradable and show an excellent mechanical stability with a large elongation at break, and even in long-time reactions no significant abrasion could be observed [11]. In addition, the PVA-alginate support matrices developed evidenced physical stability at high temperatures (>50 and <80°C).…”

Section: Discussionmentioning

confidence: 96%

“…Even though it has been mainly used for cell immobilization [8], it has been successfully applied to the immobilization of enzymepolymer composites [9,10] and enzyme aggregates [11]. The most common immobilization method is performed by mixing the biocatalyst with a solution of commercial PVA, and the promotion of gelification is accomplished by dripping the mixture over a surface, allowing for partial drying [8,12] by freezing and thawing [13] or by UV light [14], or otherwise by dripping the mixture to an extrusion solution such as boric acid or sodium sulfate to promote the cross-link between PVA molecules [15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immobilization of Naringinase in PVA–Alginate Matrix Using an Innovative Technique

Nunes

Vila-Real

Fernandes

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

A synthetic polymer, polyvinyl alcohol (PVA), a cheap and nontoxic synthetic polymer to organism, has been ascribed for biocatalyst immobilization. In this work PVA-alginate beads were developed with thermal, mechanical, and chemical stability to high temperatures (<80 degrees C). The combination of alginate and bead treatment with sodium sulfate not only prevented agglomeration but produced beads of high gel strength and conferred enzyme protection from inactivation by boric acid. Naringinase from Penicillium decumbens was immobilized in PVA (10%)-alginate beads with three different sizes (1-3 mm), at three different alginate concentrations (0.2-1.0%), and these features were investigated in terms of swelling ratio within the beads, enzyme activity, and immobilization yield during hydrolysis of naringin. The pH and temperature optimum were 4.0 and 70 degrees C for the PVA-alginate-immobilized naringinase. The highest naringinase activity yield in PVA (10%)-alginate (1%) beads of 2 mm was 80%, at pH 4.0 and 70 degrees C. The Michaelis constant (K(Mapp)) and the maximum reaction velocity (V(maxapp)) were evaluated for both free (K(Mapp) = 0.233 mM; V(maxapp) = 0.13 mM min(-1)) and immobilized naringinase (K(Mapp) = 0.349 mM; V(maxapp) = 0.08 mM min(-1)). The residual activity of the immobilized enzyme was followed in eight consecutive batch runs with a retention activity of 70%. After 6 weeks, upon storage in acetate buffer pH 4 at 4 degrees C, the immobilized biocatalyst retained 90% of the initial activity. These promising results are illustrative of the potential of this immobilization strategy for the system evaluated and suggest that its application may be effectively performed for the entrapment of other biocatalysts.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Immobilization of Naringinase in PVA–Alginate Matrix Using an Innovative Technique

Nunes

Vila-Real

Fernandes

et al. 2009

Appl Biochem Biotechnol

View full text Add to dashboard Cite

show abstract

“…superior mass transfer properties and easy separation from broth by sieves. In addition, they are prepared by a gentle immobilization method in an elastic PVA matrix known for its low toxicity, mechanical and good long-term stability and low biodegradability (Ding & Vorlop, 1995;Parascandola, Branduardi, & De Alteriis, 2006;Rebroš, Rosenberg, Mlichová, & Krištofíková, 2007). LentiKats Ò have been succesfully used for entrapment of cells, e.g.…”

Section: Introductionmentioning

confidence: 99%

Production of d-galactose using β-galactosidase and Saccharomyces cerevisiae entrapped in poly(vinylalcohol) hydrogel

et al. 2009

View full text Add to dashboard Cite

“…A novel non-aggressive method (called LentiKat 1 ) of PVAgel preparation at room temperature by means of controlled partial drying was developed recently [13,14]. Due to the lenticular shape, LentiKats 1 have the beneficial properties of both, small and large carriers, namely good diffusion properties as a result of small lens thickness and good mechanical properties and simple separation, typical for large carriers [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of yeast cells in PVA particles for beer fermentation

Bezbradica¹,

Obradović

Leskosek-Cukalovic³

et al. 2007

Process Biochemistry

View full text Add to dashboard Cite

PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production

Cited by 27 publications

References 17 publications

Immobilization of Naringinase in PVA–Alginate Matrix Using an Innovative Technique

Immobilization of Naringinase in PVA–Alginate Matrix Using an Innovative Technique

Production of d-galactose using β-galactosidase and Saccharomyces cerevisiae entrapped in poly(vinylalcohol) hydrogel

Immobilization of yeast cells in PVA particles for beer fermentation

Contact Info

Product

Resources

About