Putative virulence properties of Aeromonas strains isolated from food, environmental and clinical sources in Italy: A comparative study

Ottaviani, Donatella; Parlani, Chiara; Citterio, Barbara; Masini, Laura; Leoni, Francesca; Canonico, Cristina; Sabatini, Luigia; Bruscolini, F.; Pianetti, A.

doi:10.1016/j.ijfoodmicro.2010.11.020

Cited by 111 publications

(104 citation statements)

References 39 publications

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“…The heterogeneity in the distribution of the toxin genes among the Aeromonas spp. have earlier been reported by several workers (Albert et al, 2000;Sechi et al, 2002;Ottaviani et al, 2011) which are in agreement with our findings. All the MPCR positive Aeromonas isolates were positive for at least one toxin gene.…”

Section: Discussionsupporting

confidence: 94%

“…In agreement with this, Bin Kingombe et al (2010) also reported that the majority of the food-borne isolates belonged to A. caviae complex, A. hydrophila complex and A. sobria complex. Ottaviani et al (2011) also reported that A. hydrophila and A. sobria have been frequently isolated from food and environmental samples, which supported our findings. Although the prevalence of other Aeromonas isolates such as A. jandaei, A. veronii, A. schubertii have been observed in this study the frequency of occurrence was less as reported earlier by Janda and Abott (2010).…”

Section: Discussionsupporting

confidence: 92%

“…The overall prevalence of the alt, act/hlyA/aer and ast genes was recorded to be 89.8%, 72.9% and 20%, respectively. In disagreement with some of the earlier reports (Sen and Rodger, 2004;Balsalobre et al, 2009) but in accordance with the others (Pablos et al, 2009;Bin Kingombe et al, 2010;Ottaviani et al, 2011;Pinto et al, 2012), the alt gene was found to be dominant among the MPCR positive isolates of Aeromonas spp. Balsalobre et al (2009) reported that 70.7%, 97.6% and 26.8% of the A. hydrophila strains tested were positive for act/hlyA/aer, ast and alt genes, respectively, and 2.4% and 36.6% of the A. jandaei strains were positive for act/hlyA/aer complex and alt genes.…”

Section: Discussioncontrasting

confidence: 67%

“…as A. caviae complex, 38% as A. hydrophila complex and 24% as A. sobria complex, and 23 % as other aeromonads by a multiplex PCR targeting ast, alt and act enterotoxin genes. However, there existed a difference in the prevalence level of the toxin genes with the earlier reports (Ottaviani et al, 2011;Pinto et al, 2012). The difference in the prevalence of the toxin genes among the Aeromonas spp.…”

Section: Discussionmentioning

confidence: 52%

“…All the MPCR positive Aeromonas isolates were positive for at least one toxin gene. Ottaviani et al (2011) also reported that 96.4% of the food-borne Aeromonas isolates were positive for at least one toxin genes. However, Pablos et al (2009) reported that all the Aeromonas isolates tested in their study were positive for alt gene, which is at par with our findings.…”

Section: Discussionmentioning

confidence: 98%

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Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Hussain¹,

Jeyasekaran²,

Shakila³

et al. 2013

AJAFST

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A total of 103 representative colonies from 53 healthy and diseased food fish samples were tested for the incidence of Aeromonas spp. Of which 57% of colonies were positive for Aeromonas spp. by the multiplex PCR (MPCR). On an average, the prevalence was found to be 66% with highest incidence in diseased fishes (76%). About 48% of healthy fish samples were observed to contain Aeromonas. The biomoleculoar identification revealed that the selected virulent hemolytic and enterotoxigenic genes appeared in four different patterns viz. alt; act//hlyA/aer; alt and act//hlyA/aer; ast, alt and act//hlyA/aer, among the MPCR positive isolates of Aeromonas spp. The overall prevalence of the toxin genes alt, act/hlyA/aer and ast among the MPCR positive Aeromonas isolates was found to be 89.8%, 72.9% and 20%, respectively. The biochemical confirmation of the MPCR positive Aeromonas isolates revealed different species in the tested samples belonging to A. hydrophila, A. sobria, A. caviae, A. jandaei, A. veronii, A. schubertii and A. trota and the dominant species was found to be A. hydrophila (48%).

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 98%

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Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Hussain¹,

Jeyasekaran²,

Shakila³

et al. 2013

AJAFST

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Detection and distribution of putative virulence associated genes inAeromonasspecies from freshwater and wastewater treatment plant

Igbinosa

Okoh

2013

J. Basic Microbiol.

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The detection of genes responsible for Aeromonas virulence is a vital tool in establishing the potential pathogenicity of the bacteria, as these virulence genes may act alone or in synergy in the establishment of infections. Freshwater and wastewater mixed liquor samples were collected from Kat river and Fort Beaufort wastewater treatment plant in the Eastern Cape Province of South Africa. Polymerase chain reaction was utilized for the amplification of the different genes coding for virulence. All virulence associated genes screened (alt, lip, fla, aer, ast, hlyA) were detected in at least one Aeromonas isolates. In fresh water sample, virulence genes were distributed as follows: lip (67%), aer (43%), alt (33%), fla (62%), ast (10%), and hlyA (86%), while in wastewater samples the occurrence were as follows: lip (92%), aer (21%), alt (54%), fla (83%), ast (29%), and hlyA (88%). The presence of these virulence genes in environmental Aeromonas isolates is of concern to public health as these organisms are potential pathogens in the environment and the virulence determinants could be transferred to aquatic organisms and humans by one mechanism or the other.

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Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources

et al. 2014

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Aeromonas hydrophila is an aquatic bacterium responsible for several human illnesses. The aim of this work was to investigate the survival ability and virulence expression of two strains from different sources (fish, strain 87 and surface water, strain LS) maintained in a seawater microcosm. The strains were analyzed for the total and viable bacterial counts, adhesion ability to Hep-2 cells and aerA gene expression by qPCR throughout the experiment (35 days). Both strains reached a putative VBNC state and lost adhesive properties but exhibited a different behavior in the expression of aerA. This could be due to the different origin of the two strains; the former adapted to a habitat rich of nutrient and the latter already used to survive in a more hostile environment. Moreover, our results indicate that the quantitative determination of aerA mRNA can be a useful indicator of virulence expression under stress conditions.

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Putative virulence properties of Aeromonas strains isolated from food, environmental and clinical sources in Italy: A comparative study

Cited by 111 publications

References 39 publications

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Detection and distribution of putative virulence associated genes inAeromonasspecies from freshwater and wastewater treatment plant

Effect of starvation on survival and virulence expression of Aeromonas hydrophila from different sources

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