Putative Rabbit Conjunctival Epithelial Stem/Progenitor Cells Preferentially Reside in Palpebral Conjunctiva

Su, Li; Cui, Hongping; Xu, Chong Rui; Xie, Xiuwen; Chen, Qun; Gao, Xi‐Cun

doi:10.3109/02713683.2011.593727

Cited by 9 publications

(7 citation statements)

References 14 publications

(12 reference statements)

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“…We confirmed our results with another important characteristic of stem cells, which was their potential to remain highly proliferative. These results are also consistent with another study (Su et al, 2011), reporting that rabbit palpebral epithelial cells grow faster and have more proliferative cells (likely originating from cancer stem cells with higher in vitro colony forming rates than the other parts of the conjunctiva).…”

Section: Discussionsupporting

confidence: 82%

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

Xie

Cui

2015

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Injury and deficiency of the lacrimal duct epithelium (LDE) can lead to a variety of lacrimal diseases. The purpose of this study was to characterize potential candidate cells for constructing a tissue-engineered LDE. Methods: Different areas of the conjunctiva and lacrimal duct tissue were removed from male adult New Zealand white rabbits for histological evaluation. Hematoxylin and eosin staining and immunohistochemical staining of cytokeratin AE1+AE3, cytokeratin 4, Ki-67, and MUC5AC were observed by light microscopy. The surface morphologies of different epithelial tissues and cellular structures were examined using field-emission scanning electron microscopy and transmission electron microscopy. Epithelial cells were isolated from tissues and identified by specific markers. In vitro, proliferative ability and Western blot analyses of the proliferating cell nuclear antigen (PCNA) of different epithelial cells cultured in identical environments were investigated and compared. Results: Histologically, the epithelial specific markers, cytokeratin AE1+AE3 and cytokeratin 4, were expressed in the conjunctiva epithelium and the LDE. Notably, highly proliferative cells stained with Ki-67 were concentrated under the epithelium in a dome structure of the posterior palpebral conjunctiva. Differentiated goblet cells were also found to a lesser extent in this region. Primary palpebral and fornical conjunctival epithelial cells (PFCECs), bulbar conjunctival epithelial cells (BCECs), and lacrimal duct epithelial cells (LDECs) were successfully separated from tissues. In vitro, rabbit PFCECs and LDECs grew faster and expressed more PCNA than BCECs. Conclusions: PFCECs are anatomically similar to LDECs. They also have similar morphological characteristics, immune phenotypes, and proliferation features. PFCECs are therefore potential candidate cells to replace LDECs in tissue engineering to treat lacrimal duct diseases.

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Section: Discussionsupporting

confidence: 82%

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

Xie

Cui

2015

J. Zhejiang Univ. Sci. B

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“…More recently, a study of the location of long-term BrdU label-retaining cells of the rabbit conjunctiva, reported that the BrdU-labeled cells appeared to be concentrated at the mucocutaneous junction with the authors suggesting that epithelial stem/progenitor cells at the mucocutaneous junction serve as the source of transient amplifying cells that migrate towards the fornix (Su et al, 2011). An even more recent study of human conjunctival epithelium by Stewart et al studied location of stem cell-like cells using colony-forming efficiency, expression of stem cell markers ABCG2, ΔNp63, and Hsp70 in cultures from multiple regions of the conjunctiva, as well as ABCG2 localization in fixed tissue.…”

Section: Location Of Conjunctival Stem Cells and Goblet Cell Lineagementioning

confidence: 99%

Goblet cells of the conjunctiva: A review of recent findings

Gipson

2016

Progress in Retinal and Eye Research

193

127

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Goblet cells within the conjunctival epithelium are specialized cells that secrete mucins onto the surface of the eye. Recent research has demonstrated new characteristics of the cells, including factors influencing their differentiation, their gene products and their functions at the ocular surface. The following review summarizes the newly discovered aspects of the role of Spdef, a member of the Ets transcription factor family in conjunctival goblet cell differentiation, the newly discovered goblet cell products including claudin2, the Wnt inhibitor Frzb, and the transmembrane mucin Muc16. The current concepts of conjunctival goblet cell function, including debris removal and immune surveillance are reviewed, as are changes in the goblet cell population in ocular surface diseases. Major remaining questions regarding conjunctival cell biology are discussed.

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“…16,41 -44 The high MPI that we found in the fornical conjunctiva can probably be explained, as we mentioned above, by the mixed collection of conjunctival cells from the inferior tarsal -bulbar -fornix. The mucocutaneous junction of the rabbit eyelid 45,46 and the rat palpebral conjunctiva 47 have also been postulated as sources of replacement for the conjunctival epithelial cells, suggesting that the location of dividing cell populations might vary between species. In addition, stem cells with high in vitro proliferative potential are usually located in the thicker epithelial zones.…”

Section: Articlesmentioning

confidence: 99%

Topographical distribution and characterization of epithelial cells and intraepithelial lymphocytes in the human ocular mucosa

et al. 2012

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The conjunctiva plays a key role in the protection of the ocular surface by initiating and regulating immune responses. In this study, we analyze the relative proportion of intraepithelial lymphocytes (IELs), apoptotic cells, and proliferative state in three different topographical regions of the normal human conjunctiva. Superior tarsal, superior bulbar, and inferior tarsal-bulbar-fornical conjunctival cells were collected by brush cytology from 63 healthy donors. Flow cytometry analysis showed higher levels of CD3⁺ and CD8⁺ IELs in both upper tarsal and bulbar conjunctiva than in the inferior tarsal-bulbar-fornix, where the CD19⁺ B cells were increased. For all zones two different cell populations (by cell size and complexity) were present in the apoptosis assay. The more complex cells were reduced within the inferior tarsal-bulbar-fornix when compared with the superior bulbar and tarsal areas. Less complex cells were more predominant in the inferior conjunctiva and were mainly alive. The mean proliferation index of the conjunctival epithelium was significantly lower in the superior bulbar conjunctiva than in superior tarsal and inferior fornical conjunctivas. These findings suggest that each topographical zone from normal human conjunctiva has a unique profile of immunophenotype, viability, and proliferative state that could be related to a differentiated regional functionality.

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Putative Rabbit Conjunctival Epithelial Stem/Progenitor Cells Preferentially Reside in Palpebral Conjunctiva

Cited by 9 publications

References 14 publications

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

潜在候选细胞构建组织工程的泪道上皮: 兔的组织学和细胞学研究

Goblet cells of the conjunctiva: A review of recent findings

Topographical distribution and characterization of epithelial cells and intraepithelial lymphocytes in the human ocular mucosa

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