Putative pacemakers of crayfish show clock proteins interlocked with circadian oscillations

Escamilla‐Chimal, Elsa G.; Velázquez-Amado, Rosa María; Fiordelisio, Tatiana; Fanjul‐Moles, María Luisa

doi:10.1242/jeb.047548

Cited by 24 publications

(28 citation statements)

References 36 publications

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“…The presence of these cell bodies in the locus where the clock proteins PER, TIM and CLOCK have been reported (cluster 6 and TIM in cluster 17) [35] and the CHH interaction in the perikarya and nuclei suggest a relationship between the clock transcription-translation feedback loop and CHH, as has been reported for other neuropeptides [36].…”

Section: Resultsmentioning

confidence: 52%

Putative Pacemakers in the Eyestalk and Brain of the Crayfish Procambarus clarkii Show Circadian Oscillations in Levels of mRNA for Crustacean Hyperglycemic Hormone

et al. 2013

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Crustacean hyperglycemic hormone (CHH) synthesizing cells in the optic lobe, one of the pacemakers of the circadian system, have been shown to be present in crayfish. However, the presence of CHH in the central brain, another putative pacemaker of the multi-oscillatory circadian system, of this decapod and its circadian transcription in the optic lobe and brain have yet to be explored. Therefore, using qualitative and quantitative PCR, we isolated and cloned a CHH mRNA fragment from two putative pacemakers of the multi-oscillatory circadian system of Procambarus clarkii, the optic lobe and the central brain. This CHH transcript synchronized to daily light-dark cycles and oscillated under dark, constant conditions demonstrating statistically significant daily and circadian rhythms in both structures. Furthermore, to investigate the presence of the peptide in the central brain of this decapod, we used immunohistochemical methods. Confocal microscopy revealed the presence of CHH-IR in fibers and cells of the protocerebral and tritocerebal clusters and neuropiles, particularly in some neurons located in clusters 6, 14, 15 and 17. The presence of CHH positive neurons in structures of P. clarkii where clock proteins have been reported suggests a relationship between the circadian clockwork and CHH. This work provides new insights into the circadian regulation of CHH, a pleiotropic hormone that regulates many physiological processes such as glucose metabolism and osmoregulatory responses to stress.

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Section: Resultsmentioning

confidence: 52%

Putative Pacemakers in the Eyestalk and Brain of the Crayfish Procambarus clarkii Show Circadian Oscillations in Levels of mRNA for Crustacean Hyperglycemic Hormone

et al. 2013

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“…We hypothesise that a similar mechanism could be acting in crayfish. This hypothesis is based on recent reports from our laboratory in which we proposed that the behavioural pattern of the molecular machinery of P. clarkii might be similar to that of Drosophila (Escamilla-Chimal et al 2010). It is possible that in crayfish, as in the fly (Weber et al 2011), a low-temperature pulse during the early subjective night may induce increasing levels of PER and accelerate the formation of PER-TIM heterodimers.…”

Section: Discussionmentioning

confidence: 89%

“…This network does not simply set the rhythm using a single central master clock, but modulates the circadian parameters of each oscillator system via phase-locking or entrainment. In the case of the photic entrainment of the activity rhythm, the recent identification of pathways and mediators of protocerebral structures (Strauss and Dircksen 2010) that also express clock proteins (Escamilla-Chimal et al 2010) appears to offer clues to the pacemakers responsible for this endogenous activity. Although the process of photo-entrainment of the crayfish activity rhythm has been widely studied and elucidated, the temperature-synchronisation process in crayfish remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Temperature pulses synchronise the crayfish locomotor activity rhythm

Palma-Anzures

Prieto-Sagredo

Fanjul‐Moles

2012

Biological Rhythm Research

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Unrestrained crayfish maintained in the dark exhibit a unimodal circadian activity that freely runs with a period of approximately 24 h rhythm that is able to entrain light-dark cycles. Although the activity rhythm has been shown to compensate for temperature, its potential for temperature synchronisation is unknown. In this work, we analysed the effects of cold-water pulses on crayfish locomotor activity. The crayfish were maintained under a 12:12 light-dark (LD) cycle and 30-min cold (58C) water pulses were applied at three different circadian times (CTs). The crayfish were subsequently kept in darkness. Cold-water pulses applied at the beginning of the subjective night produced phase advances whereas pulses applied at the end of the subjective night produced phase delays. The magnitudes of the phase shifts were similar to those produced by light pulses but the direction was a mirror image. These findings suggest that the temperatureand light-synchronising pathways differ in crayfish.

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“…The cellular and molecular composition of the circannual pacemaker in Decapods is unknown. However, some information is available for circadian pacemakers and the involvement of melatonin in their synchronization with environmental rhythms (Escamilla-Chimal et al 2010;Strauss & Dircksen 2010;Fanjul-Moles 2014). This knowledge may help to identify the circannual system because the circadian rhythm is assumed to be the basic unit of rhythms with longer periods (Strauss & Dircksen 2010).…”

Section: Discussionmentioning

confidence: 99%

Bimodal annual reproductive pattern in laboratory-reared marbled crayfish

Vogt

2015

Invertebrate Reproduction & Development

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The marbled crayfish, Procambarus fallax f. virginalis, is a new research model and potent invader of aquatic ecosystems that reproduces by obligatory parthenogenesis. My data show that in captivity, it can reproduce throughout the year. However, when kept at constant 20°C and natural photoperiod there were two prominent spawning maxima, one before the spring equinox and another one before the fall equinox. If temperature fluctuated between~15°C in winter and~25°C in summer, the first maximum was shifted beyond the spring equinox and the second maximum was shifted closer towards the fall equinox. These results indicate a bimodal annual rhythm for the reproduction in marbled crayfish that can be modulated by temperature. Comparison with P. fallax suggests that this rhythmicity was inherited from its sexually reproducing mother species. Potential consequences of my laboratory findings for wild populations of marbled crayfish in temperate and tropical regions are discussed. Since marbled crayfish can be kept in captivity for up to four years under a broad range of conditions, they offer the possibility to investigate endogenous circannual rhythms and their entrainment by environmental zeitgebers in decapod crustaceans.

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Putative pacemakers of crayfish show clock proteins interlocked with circadian oscillations

Cited by 24 publications

References 36 publications

Putative Pacemakers in the Eyestalk and Brain of the Crayfish Procambarus clarkii Show Circadian Oscillations in Levels of mRNA for Crustacean Hyperglycemic Hormone

Putative Pacemakers in the Eyestalk and Brain of the Crayfish Procambarus clarkii Show Circadian Oscillations in Levels of mRNA for Crustacean Hyperglycemic Hormone

Temperature pulses synchronise the crayfish locomotor activity rhythm

Bimodal annual reproductive pattern in laboratory-reared marbled crayfish

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