Putative Novel Genotype of Avian Hepatitis E Virus, Hungary, 2010

Bányai, Krisztiàn; Tóth, Ágnes; Ivánics, Éva; Gianni, Robert; Szentpáli-Gavallér, Katalin; Dán, Ádám

doi:10.3201/eid1808.111669

Cited by 39 publications

(32 citation statements)

References 14 publications

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“…Accordingly, the isolates from broiler breeders and day-old chicks investigated in this study grouped within genotype 3, which contains predominantly European isolates. Recently, based on phylogenetic analysis of the whole genome, a new genotype of avian HEV, consisting of a Hungarian and a Taiwanese strain, has been proposed (Bányai et al, 2012;Hsu & Tsai, 2014). The close phylogenetic relationship between those two viruses was supported in this study by analysing helicase nucleic acid sequences but not by capsid nucleic acid sequences, arguing for further analyses.…”

Section: Discussionmentioning

confidence: 70%

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Subclinical circulation of avian hepatitis E virus within a multiple-age rearing and broiler breeder farm indicates persistence and vertical transmission of the virus

Troxler

Pać²,

Prokofieva

et al. 2014

Avian Pathology

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In a prospective longitudinal study, a broiler breeder flock and its progeny were monitored for the presence of avian hepatitis E virus (HEV) RNA and antibodies. The flock was part of a multiple-age farm where the presence of avian HEV with clinical signs (increased mortality and decreased egg production) was demonstrated in several previous production cycles. Samples were taken twice at the rearing site and several times at the production site from broiler breeders including cockerels and day-old chicks. The samples were investigated by conventional and real-time reverse transcriptase-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and histological methods. At all time points, samples from the hens were positive for avian HEV RNA. The birds did not show any clinical signs, even though histopathological lesions of non-specific aetiology in the liver and spleen could be demonstrated. A significant increase in the number of positive birds and viral load was seen in week 45, in accordance with an increase in antibody titres. In comparison, cockerels investigated in week 62 tested negative by RT-PCR and ELISA. Avian HEV RNA was also detected in day-old chicks hatched from eggs laid in week 25, indicating vertical transmission. All partial helicase and capsid sequences retrieved within this study clustered together and were identical to previous sequences obtained from the same multiple-age farm. In conclusion, avian HEV persisted on the farm over years and circulated between the rearing and the production sites without causing any clinical signs although high viral loads in the adult hens were observed.

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Section: Discussionmentioning

confidence: 70%

“…Recently, avian HEV infections have also been described in chicken flocks from Russia, Korea and China (Zhao et al, 2010;Kwon et al, 2012;Sprygin et al, 2012). In Hungary and Taiwan, an apparently new genotype has been found (Bányai et al, 2012;Hsu & Tsai, 2014).…”

Section: Please Scroll Down For Articlementioning

confidence: 96%

Subclinical circulation of avian hepatitis E virus within a multiple-age rearing and broiler breeder farm indicates persistence and vertical transmission of the virus

Troxler

Pać²,

Prokofieva

et al. 2014

Avian Pathology

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“…Avian picobirnaviruses (AvPBV) genogroup I in Brazilian broiler chickens and distinct group of turkey origin picobirnavirus have been identified (Ribeiro Silva et al, 2014;Day and Zsak, 2014). A novel avian hepatitis E virus (aHEV) genotype IV has been identified in Hungary and Taiwan (Banyai et al, 2012;Hsu and Tsai, 2014). CPNV associated with transmissible viral proventriculitis in broiler chicken was identified as a novel avian birnavirus, representing a distinct genetic cluster within the Birnaviridae family (Guy et al, 2011).…”

Section: Classical Methods For Study Of Virus Diversitymentioning

confidence: 99%

Next generation sequencing technologies: Tool to study avian virus diversity

Kapgate¹,

Barbuddhe²,

Kumanan³

2015

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Summary. -Increased globalisation, climatic changes and wildlife-livestock interface led to emergence of novel viral pathogens or zoonoses that have become serious concern to avian, animal and human health. High biodiversity and bird migration facilitate spread of the pathogen and provide reservoirs for emerging infectious diseases. Current classical diagnostic methods designed to be virus-specific or aim to be limited to group of viral agents, hinder identifying of novel viruses or viral variants. Recently developed approaches of next-generation sequencing (NGS) provide culture-independent methods that are useful for understanding viral diversity and discovery of novel virus, thereby enabling a better diagnosis and disease control. This review discusses the different possible steps of a NGS study utilizing sequence-independent amplification, high-throughput sequencing and bioinformatics approaches to identify novel avian viruses and their diversity. NGS lead to the identification of a wide range of new viruses such as picobirnavirus, picornavirus, orthoreovirus and avian gamma coronavirus associated with fulminating disease in guinea fowl and is also used in describing viral diversity among avian species. The review also briefly discusses areas of viral-host interaction and disease associated causalities with newly identified avian viruses.

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“…Tab. 1) [14,15,142,143]. Inwieweit der Vorschlag sinnvoll ist, aviäre HEV-Sequenzen distinkten Genotypen zuzuordnen, ist unklar [14].…”

Section: Aviäre Hevunclassified