Putative estrogen receptor β and α mRNA expression in male and female rhesus macaques

Pau, Candace Y.; Pau, K.-Y. Francis; Spies, Harold G.

doi:10.1016/s0303-7207(98)00197-x

Cited by 111 publications

(83 citation statements)

References 27 publications

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“…Although in both investigations a very limited number of monkeys were studied, labeling for ER-β mRNA was observed in the surface epithelium, glandular epithelium, and stromal cells. Weak labeling in the smooth muscle cells of the myometrium was noted in the study by Pelletier et al (1999), but Pau et al (1998) found only ER-α mRNA in the myometrium. This difference could possibly be explained by the age or phase of cycle of the animal(s) used in the investigations.…”

Section: Hormone Receptors and Factorsmentioning

confidence: 71%

“…This receptor occurs in a wide range of tissues in several species, including macaques (Pelletier et al 1999) and man (Mosselman et al 1996). Pau et al (1998) and Pelletier et al (1999) investigated the uterus using reverse transcription-polymerase chain reaction and in situ hybridization, respectively, to localize ER-β mRNA expression. Although in both investigations a very limited number of monkeys were studied, labeling for ER-β mRNA was observed in the surface epithelium, glandular epithelium, and stromal cells.…”

Section: Hormone Receptors and Factorsmentioning

confidence: 99%

See 1 more Smart Citation

The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

Esch¹,

Cline²,

Buse³

et al. 2008

Toxicol Pathol

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The macaque endometrium undergoes dramatic morphologic and functional changes during the menstrual cycle that are nearly identical to those of the human endometrium. The sequential events that take place in the endometrium are mainly driven by the ovarian steroids and their respective receptors. To be able to interpret the changes and effects induced by mammalian or synthetic hormones and other compounds that could have influence on the hormonal status of the animal, a thorough knowledge of the anatomy, physiology, and histology of the cyclic hormone-mediated processes within the endometrium is indispensable. In this paper we give an overview of uterine growth and development, anatomy, basic histology, aging, spontaneous pathology, and the techniques to study the endometrium in-life. In addtion, a comprehensive description of the receptor-mediated, hormonedriven morphological changes during the menstrual cycle in the cynomolgus monkey (Macaca fascicularis) is given. Where possible, differences between the macaque and human endometria are discussed.Keywords: female reproduction; endometrium; histology; menstrual cycle; hormone receptors; endometrial dating; cynomolgus monkey.human. For this reason, monkey species belonging to this group are preferred as experimental models in studies of human reproduction, pharmacology, and toxicology. Especially for studies with sex hormones, synthetic steroidal compounds or compounds that interfere with the hypothalamus-pituitary-ovarian (or testicular) axis, such monkey species are predictive . The rhesus monkey (Macaca mulatta) and the cynomolgus monkey (Macaca fascicularis) are the species most often used for these purposes. In our laboratories, purpose-bred cynomolgus monkeys are used for such studies. Besides their resemblance in menstrual cycle physiology, these monkeys are relatively small and easy to handle.Both the human and the macaque monkey endometria undergo dramatic morphologic and functional changes during the menstrual cycle. The sequential events that take place in the endometrium are mainly driven by the ovarian steroids estradiol and progesterone and their respective receptors.To be able to interpret the changes and effects induced by mammalian or synthetic hormones and other compounds that could have influence on the hormonal status of the animal, or that interfere with the regulatory processes within the endometrium, a thorough knowledge of the anatomy, physiology, and histology of cyclic hormone-mediated processes within the endometrium is indispensable.In this paper we give a brief overview of endometrial growth and development, anatomy, basic histology, and the effects of aging, and we briefly discuss the spontaneous pathology of the endometrium of macaques. The receptor-mediated,

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Section: Hormone Receptors and Factorsmentioning

confidence: 71%

Section: Hormone Receptors and Factorsmentioning

confidence: 99%

The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

Esch¹,

Cline²,

Buse³

et al. 2008

Toxicol Pathol

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“…Here, we have shown that there is ER mRNA expression in hypothalamus and pituitary tissues of newborn piglets. High amounts of ER transcripts were previously found in the pituitary of the pre-pubertal rat (Mitchner et al 1998, Wilson et al 1998, human (Shupnik et al 1998), and rhesus monkey (Pau et al 1998), whereas levels in mouse pituitary appeared to be low or nearly undetectable (Couse et al 1997). It has been reported that the expression of ER is high in rat and human fetal hearts (Saunders 1998).…”

Section: Discussionmentioning

confidence: 95%

Feeding daidzein to late pregnant sows influences the estrogen receptor beta and type 1 insulin-like growth factor receptor mRNA expression in newborn piglets

Ren¹,

Kuhn²,

Wegner³

et al. 2001

Journal of Endocrinology

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The present study was undertaken to determine the tissue-specific expression of estrogen receptor beta (ER ), and the effects of a daidzein supplement to the diet of pregnant sows on the expression of ER , and type 1 insulin-like growth factor receptor (IGF-1R) genes in newborn piglets by using semi-quantitative RT-PCR. Eight sows received a dietary supplement of daidzein at a dosage of 8 mg per kg feed from day 85 of gestation, and six sows were used as controls. After parturition, 2 male neonatal piglets were selected from each litter for sampling. ER mRNA was detected in intestine, lung, thymus, kidney, pituitary and hypothalamus tissues, but not in heart, adrenal, skeletal muscle, liver or placental tissues. Daidzein treatment significantly increased the birth weight of male piglets and markedly reduced the level of ER mRNA in the hypothalamus, but not in the pituitary. An up-regulation of IGF-1R gene transcription was observed in skeletal muscles of newborn piglets. In addition, the IGF-1R mRNA was found to be most abundant in pituitary and hypothalamus, followed by skeletal muscle, thymus, and liver tissues in decreasing order. Our results demonstrate that (1) ER is expressed in a tissue-specific manner in newborn piglets, (2) daidzein down-regulates ER gene expression in the hypothalamus, possibly indicating central effects of daidzein, and (3) daidzein influences fetal growth associated with higher IGF-IR gene expression in skeletal muscle.

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“…DNA size marker was the 123 bp DNA ladder from GIBCO BRL-Life Technologies. Positive control size markers were also generated from total RNA isolated from an appropriate tissue (data not shown), using lung for ER (Maruyama et al 1998) and testis for both ER (Pau et al 1998) and AR (Wilson & McPhaul 1996), and/or from a cDNA plasmid source (human AR cDNA kindly provided by Dr Marco Marcelli (Baylor College of Medicine and VA Medical Center, Houston, TX, USA) and human ER cDNA provided by Dr Pierre Chambon (CNRS, Illkirch Cedex, France)). Negative controls routinely included a no-RT reaction (data not shown).…”

Section: Rna Isolation and Relative Rt-pcr Semi-quantitative Analysismentioning

confidence: 99%

Osteoblast differentiation influences androgen and estrogen receptor-alpha and -beta expression

Wiren

Evans²,

Xw³

2002

Journal of Endocrinology

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Significant levels of estrogen and androgens circulate in men and women, and both play an important role in bone metabolism. While it is well established that either estrogen or androgen replacement therapy is effective at ameliorating bone loss associated with hypogonadism, recent evidence nevertheless suggests that estrogen and androgens have distinct molecular actions on the skeleton. In this study, we have employed normal rat calvarial osteoblast cultures to characterize relative expression profiles of estrogen (ER and ER ) and androgen receptors (AR) during osteoblast differentiation. Normal osteoblast cultures can proceed through in vitro differentiation with distinct stages of proliferation, matrix maturation and mineralization in the appropriate differentiation medium containing ascorbic acid. Expression profiles of AR, ER and ER in primary cultures during osteoblast differentiation were characterized both by semi-quantitative relative RT-PCR and by Western analysis. In cultures induced to differentiate by growth in the presence of ascorbic acid, the expression profile for each receptor was unique during the course of differentiation. ER levels were elevated during matrix maturation and then declined during mineralization. ER expression was relatively constant throughout differentiation, exhibiting more constitutive expression. In contrast, AR levels were lowest during proliferation, and then increased throughout differentiation with highest levels in the most mature mineralizing cultures. Since steroid hormone action is generally mediated by specific cognate receptors, these results suggest that androgen actions may target cells during the mineralization stage of osteoblast differentiation, while estrogen action through either receptor isoform is more likely to affect osteoblasts earlier during matrix maturation. Interestingly, sex steroid receptor expression profiles did not exhibit the same patterns of regulation if osteoblast cultures were grown without ascorbic acid in medium that did not support extracellular matrix deposition. Thus, sex steroids may distinctly influence skeletal health by differential modulation of function during osteoblast differentiation.

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Putative estrogen receptor β and α mRNA expression in male and female rhesus macaques

Cited by 111 publications

References 27 publications

The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

The Macaque Endometrium, with Special Reference to the Cynomolgus Monkey (Macaca fascicularis)

Feeding daidzein to late pregnant sows influences the estrogen receptor beta and type 1 insulin-like growth factor receptor mRNA expression in newborn piglets

Osteoblast differentiation influences androgen and estrogen receptor-alpha and -beta expression

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