Pusillimonas thiosulfatoxidans sp. nov., a thiosulfate oxidizer isolated from activated sludge

Abstract

A Gram-stain-negative, motile bacterium, designated strain YE3 T , was isolated from activated sludge obtained from a municipal wastewater treatment plant in Daejeon Metropolitan City, Republic of Korea. The cells were oxidase-and catalasepositive, and grew under aerobic conditions at 10-40 C (optimum, 30 C), with 1.0-8.0 % (w/v) NaCl (1.0 %) and at pH 5.5-9.0 (pH 7.0). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain YE3 T was most closely related to Pusillimonas harenae KACC 14927 T (98.2 % sequence similarity) and Pusillimonas ginsengisoli KCTC 22046 T (98.0 %). DNA-DNA relatedness values for strain YE3 T and P. harenae KACC 14927 T , P. ginsengisoli KCTC 22046 T and P. soli KCTC 22455 T were 28.7±2.27 %, 21.3±1.16 %, and 14.0±0.67 %, respectively. The genomic G+C content of the type strain YE3 T was 59.3 mol%, as determined by whole-genome sequencing. The dominant fatty acids were C 16 : 0 (39.2 %) and C 17 : 0 cyclo (37.5 %). The major polar lipids of strain YE3 T were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Two aminophospholipids and four unidentified lipids were also detected. Furthermore, strain YE3 T was able to oxidize thiosulfate under heterotrophic conditions. Based on the phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain YE3 T represents a novel species of the genus Pusillimonas, for which the name Pusillimonas thiosulfatoxidans sp. nov. is proposed. The type strain is YE3 T (=KCTC 62737 T =NBRC 113113 T).

Molecular mechanism of sulfur chemolithotrophy in the betaproteobacterium Pusillimonas ginsengisoli SBSA

“…Among the above-mentioned genera, the mechanism of sulfur chemolithotrophy is well known in Advenella. However, one thiosulfate-oxidizing species of Pusillimonas has been isolated recently from activated sludge, although it does not harbour any homologues of structural sox genes in its genome [44]. The present investigation brings to the fore the establishment and molecular characterization of sulfur chemolithotrophy in the genus Pusillimonas.…”

Dynamic Effect of Operational Regulation on the Mesophilic BioMethanation of Grape Marc

“…It can also be noted from Figure 4 that aerobic Hydrogenophaga and Pusillimonas genera from the Proteobacteria phylum displayed trends of increasing microbial richness from the initial values, despite depleting oxygen levels because of chemoorganotrophic or chemolithoautrophic competence, allowing for oxidation of hydrogen as energy source and CO 2 as carbon source [79][80][81]. During the mono-digestion of GM, the CO 2 concentration reached a peak of 23.6% of total biogas volume on day 5 of digestion, lowering to 11.4% and 11.0%, on day 20 and 42, respectively.…”

“…Molecules 2021, 26, x FOR PEER REVIEW these important microbial groups mediate the upstream metabolic stages syntrophic electron flow on to Archaea, there may be an accumulation of fer products which may in turn inhibit methane production, resulting in a decrea trend (Figure 2) [12]. It can also be noted from Figure 4 that aerobic Hydrogenophaga and Pusillim era from the Proteobacteria phylum displayed trends of increasing microbial rich the initial values, despite depleting oxygen levels because of chemoorgano chemolithoautrophic competence, allowing for oxidation of hydrogen as ene and CO2 as carbon source [79][80][81]. During the mono-digestion of GM, the CO2 tion reached a peak of 23.6% of total biogas volume on day 5 of digestion, lo 11.4% and 11.0%, on day 20 and 42, respectively.…”

Molecular mechanism of sulfur chemolithotrophy in the betaproteobacterium Pusillimonas ginsengisoli

“…So far as the sulfur oxidation in Alcaligenaceae family is concerned, only members of Advenella [24], Alcaligenes [39], Bordetella [40], have the sulfur oxidation phenotype while Achromobacter despite having complete sox cluster does not have any sulfur chemotrophic attribute. However, recently one thiosulfate oxidizing species of Pusillimonas has been reported from activated sludge, does not harbors any homologs of structural sox genes in its genome [41]. To the best of our knowledge we first time bring to the fore establishment and molecular characterization of sulfur chemolithotrophy in the genus Pusillimonas .…”