Pushing the limits of microfluidic droplet production efficiency: engineering microchannels with seamlessly implemented 3D inverse colloidal crystals

Mashiyama, Shota; Hemmi, Runa; Sato, Takeru; Kato, Atsuya; Taniguchi, Tatsuo; Yamada, Masumi

doi:10.1039/d3lc00913k

Cited by 1 publication

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“…Specifically, here we focused on porous substrates with interconnected micropores . Recently, studies have reported the fabrication of sponge-like matrices using PDMS as the material and the incorporation of porous structures into microfluidic devices. , We anticipated that a porous substrate with high-density micropores on the surface, once integrated into microchannel structures, could function as a highly efficient drainage system to extract the flow from a particle/cell suspension. From these viewpoints, we developed a new concept of a sheath flow generator, as shown in Figure .…”

Section: Introductionmentioning

confidence: 99%

High-Density Microporous Drainage-Integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting Systems

Hayashi,

Hemmi,

Saito

et al. 2024

Anal. Chem.

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Tremendous efforts have been made to develop practical and efficient microfluidic cell and particle sorting systems; however, there are technological limitations in terms of system complexity and low operability. Here, we propose a sheath flow generator that can dramatically simplify operational procedures and enhance the usability of microfluidic cell sorters. The device utilizes an embedded polydimethylsiloxane (PDMS) sponge with interconnected micropores, which is in direct contact with microchannels and seamlessly integrated into the microfluidic platform. The high-density micropores on the sponge surface facilitated fluid drainage, and the drained fluid was used as the sheath flow for downstream cell sorting processes. To fabricate the integrated device, a new process for sponge-embedded substrates was developed through the accumulation, incorporation, and dissolution of PMMA microparticles as sacrificial porogens. The effects of the microchannel geometry and flow velocity on the sheath flow generation were investigated. Furthermore, an asymmetric lattice-shaped microchannel network for cell/particle sorting was connected to the sheath flow generator in series, and the sorting performances of model particles, blood cells, and spiked tumor cells were investigated. The sheath flow generation technique developed in this study is expected to streamline conventional microfluidic cell-sorting systems as it dramatically improves versatility and operability.

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Section: Introductionmentioning

confidence: 99%

High-Density Microporous Drainage-Integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting Systems

Hayashi,

Hemmi,

Saito

et al. 2024

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

Pushing the limits of microfluidic droplet production efficiency: engineering microchannels with seamlessly implemented 3D inverse colloidal crystals

Abstract: Although droplet microfluidics has been studied for the past two decades, its applications are still limited due to the low productivity of microdroplets resulting from the low integration of planar microchannel structures.

Cited by 1 publication

References 64 publications

High-Density Microporous Drainage-Integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting Systems

High-Density Microporous Drainage-Integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting Systems

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